4.5 Semen (Additional Testing) Flashcards
Abnormal Result: Decreased Motility, normal count
Possible abnormality: Presence of _____ sperm
Work-up: Viability testing
Dead
What is the viability testing in detecting dead sperm?
Eosin-nigrosin
in Eosin-nigrosin:
what is the color of dead and viable sperm?
Dead - Red
Viable - Bluish white
Abnormal Result: Decreased Sperm count
Possible abnormality: Lack of support medium
Work-up: _________ determination / Resorsinol test
Fructose
Abnormal Result: Decreased Motility with clumping
Possible abnormality: Male _________ antibodies
Work-up:
- Incubation with male ____
- MAR
-Immunobead
Antisperm
Serum
Abnormal Result: Normal analysis but continued infertility
Possible abnormality: Female anti-sperm antibodies
Work-up: Incubation with _______ serum
Female
Abnormal Result: Normal analysis but continued infertility
Possible abnormality: Failure of ovum penetration
Work-up:
- _____________ penetration
- In _____ acrosome reaction
Hamster egg
Vitro
Abnormal Result: Normal analysis but continued infertility
Possible abnormality: Inability to penetrate partner’s midcycle cervical mucus
Work-up: ________ Mucus penetration
Cervical
Abnormal Result: Normal analysis but continued infertility
Possible abnormality: Lack of membrane integrity and viability
Work-up: _____-osmotic swelling test
Hypo
What is the test for cervical mucus penetration?
Sims-Huhner test
What is the specimen for Sims-Huhner test?
Post-coital vaginal aspirate
Immature sperm (spermatids) or leukocytes (POD-positive neutrophils)
Round cells
Formula for Round cells:
C = (N x S) / 100
What is “N”
Neutrophils or spermatids (Round cells)
Formula for Round cells:
C = (N x S) / 100
What is “100”
Mature sperm
Formula for Round cells:
C = (N x S) / 100
What is “S”
Sperm concentration
