4.5 Semen (Additional Testing) Flashcards
Abnormal Result: Decreased Motility, normal count
Possible abnormality: Presence of _____ sperm
Work-up: Viability testing
Dead
What is the viability testing in detecting dead sperm?
Eosin-nigrosin
in Eosin-nigrosin:
what is the color of dead and viable sperm?
Dead - Red
Viable - Bluish white
Abnormal Result: Decreased Sperm count
Possible abnormality: Lack of support medium
Work-up: _________ determination / Resorsinol test
Fructose
Abnormal Result: Decreased Motility with clumping
Possible abnormality: Male _________ antibodies
Work-up:
- Incubation with male ____
- MAR
-Immunobead
Antisperm
Serum
Abnormal Result: Normal analysis but continued infertility
Possible abnormality: Female anti-sperm antibodies
Work-up: Incubation with _______ serum
Female
Abnormal Result: Normal analysis but continued infertility
Possible abnormality: Failure of ovum penetration
Work-up:
- _____________ penetration
- In _____ acrosome reaction
Hamster egg
Vitro
Abnormal Result: Normal analysis but continued infertility
Possible abnormality: Inability to penetrate partner’s midcycle cervical mucus
Work-up: ________ Mucus penetration
Cervical
Abnormal Result: Normal analysis but continued infertility
Possible abnormality: Lack of membrane integrity and viability
Work-up: _____-osmotic swelling test
Hypo
What is the test for cervical mucus penetration?
Sims-Huhner test
What is the specimen for Sims-Huhner test?
Post-coital vaginal aspirate
Immature sperm (spermatids) or leukocytes (POD-positive neutrophils)
Round cells
Formula for Round cells:
C = (N x S) / 100
What is “N”
Neutrophils or spermatids (Round cells)
Formula for Round cells:
C = (N x S) / 100
What is “100”
Mature sperm
Formula for Round cells:
C = (N x S) / 100
What is “S”
Sperm concentration
An aerobic and anaerobic cultures and tests for C. trachomatis, M. hominis, and U. urealyticum
Microbial testing
Chemical testing
Test epididymis
a. More than or equal to 20mU/ejaculate
b. More than or equal to 2.4 umol/ejaculate
c. More than or equal to 52 umol/ejaculate
d. More than or equal to 200 units/ejaculate
a
Chemical testing
Test for prostatic insufficiency
a. More than or equal to 20mU/ejaculate
b. More than or equal to 2.4 umol/ejaculate
c. More than or equal to 52 umol/ejaculate
d. More than or equal to 200 units/ejaculate
b,c,d
Chemical testing
Neutral alpha-glucosidase
a. More than or equal to 20mU/ejaculate
b. More than or equal to 2.4 umol/ejaculate
c. More than or equal to 52 umol/ejaculate
d. More than or equal to 200 units/ejaculate
a
Chemical testing
Zinc
a. More than or equal to 20mU/ejaculate
b. More than or equal to 2.4 umol/ejaculate
c. More than or equal to 52 umol/ejaculate
d. More than or equal to 200 units/ejaculate
b
Chemical testing
Citric acid
a. More than or equal to 20mU/ejaculate
b. More than or equal to 2.4 umol/ejaculate
c. More than or equal to 52 umol/ejaculate
d. More than or equal to 200 units/ejaculate
c
Chemical testing
Acid phosphatase
a. More than or equal to 20mU/ejaculate
b. More than or equal to 2.4 umol/ejaculate
c. More than or equal to 52 umol/ejaculate
d. More than or equal to 200 units/ejaculate
d
In post-vasectomy analysis Involves examining a wet preparation using what microscope?
Phase contrast microscope
Test of choice to determine the sufficiency of fructose level
a. Resorcinol test
b. Mixed Agglutination Reaction (MAR)
c. Immunobed
a
A screening test, nonspecific, for Antisperm antibodies
a. Resorcinol test
b. Mixed Agglutination Reaction (MAR)
c. Immunobead
b
Confirmatory test fir antisperm antibodies
a. Resorcinol test
b. Mixed Agglutination Reaction (MAR)
c. Immunobead
c
Reagent for hypo-osmotic sweelling test/Challenge
Decreasing concentrations of NaCl solution
