2.4 Synovial Fluid (Microscopic Examination) Flashcards
In WBC count for Synovial fluid, dilutions done only on turbid or bloody fluids using?
a. 3% acetic acid
b. Saponin
c. Hypotonic saline
d. Hyaluronidase
b and c
Purpose of diluent?
Lyse RBC
In differential count for Synovial fluid, requires incubation of the fluid with?
a. 3% acetic acid
b. Saponin
c. Hypotonic saline
d. Hyaluronidase
Hyaluronidase
What component of the synovial fluid is responsible for viscosity?
Hyaluronic acid
How do you weaken the viscosity of Synovial fluid?
Hyaluronidase
Neutrophils is normally seen in synovial fluid in < ___ % of the differential
25
CLinical significant
Increase septic or crystal - induced arthritis
a. Neutrophil
b. Lymphocyte
c. Macrophage
d. Synoviocyte
a
Lymphocyte is normally seen in Synovial fluid at ___% of the differential
15
Macrophage is normally seen in synovial fluid
T or F
T
This may be multinucleated resembling a mesothelial cell
a. Neutrophil
b. Lymphocyte
c. Macrophage
d. Synoviocyte
d
Clinical significance:
Increase non-septic inflammation
a. Neutrophil
b. Lymphocyte
c. Macrophage
d. Synoviocyte
b and c
Synoviocyte is normally seen in regardless if there is clinical significant cases
T or F
T
Clinical significance: None
Normal:
Neutrophil with ingested round body
a. LE cells
b. Reiter Cell / Neutrophage
c. Ragocyte
d. Cartilage cell
a
Normal:
Neutrophil with dark granules containing immune complexes
a. LE cells
b. Reiter Cell / Neutrophage
c. Ragocyte
d. Cartilage cell
c
Normal:
Macrophage with ingested neutrophils
a. LE cells
b. Reiter Cell / Neutrophage
c. Ragocyte
d. Cartilage cell
b
Normal:
Large, multinucleated cells
a. LE cells
b. Reiter Cell / Neutrophage
c. Ragocyte
d. Cartilage cell
d
Clinical significance:
SLE
a. LE cells
b. Reiter Cell / Neutrophage
c. Ragocyte
d. Cartilage cell
a
Clinical significance
Rheumatoid arthritis
a. LE cells
b. Reiter Cell / Neutrophage
c. Ragocyte
d. Cartilage cell
c
Clinical significance:
Osteoarthritis
a. LE cells
b. Reiter Cell / Neutrophage
c. Ragocyte
d. Cartilage cell
d
Clinical significance:
Reiter’s syndrome
a. LE cells
b. Reiter Cell / Neutrophage
c. Ragocyte
d. Cartilage cell
b
Normal:
Microscopically show collagen and fibrin
a. Rice bodies
b. Fat droplets
c. Hemosiderin
a
Normal:
Refractile globules, Sudan +
a. Rice bodies
b. Fat droplets
c. Hemosiderin
b
Normal:
Inclusion within clusters of synovial cells
a. Rice bodies
b. Fat droplets
c. Hemosiderin
c
Clinical significance:
TB, Septic, and Rheumatoid arthritis
a. Rice bodies
b. Fat droplets
c. Hemosiderin
a
Clinical significance
Pigmented Villonodular synovitis
a. Rice bodies
b. Fat droplets
c. Hemosiderin
c
Clinical significance:
Trauma and chronic inflammation
a. Rice bodies
b. Fat droplets
c. Hemosiderin
b
A stain of choice for hemosiderin?
Prussian blue
