1.4 Cerebrospinal fluid (Microscopic Examination) Flashcards
WBC count normal values:
Adult ____
Neonates: ______
<5/uL
<30/uL
Clarity and recommended dilutions:
Slightly hazy: _____
Hazy:_____
Slightly cloudy:_____
Slightly Bloody: _______
Cloudy, Bloody, turbid: _______
1:10
1:20
1:100
1:200
1:10,000
How do you count in neuboer counting chamber for WBC?
Count in 5 large sq/side (Higher and Lower)
=
10 sq
Calculation for Wbc/uL:
No. of cells counted x ________ factor / No. of ________ counted x volume of each square (____ uL)
dilution
squares
0.1
If No. of squares are counted in 10, the denominator in WBC/uL formula is 1
T or F
T
What is the diluent use in dilution for WBC count?
3% Acetic acid (HAc)
Total cell count uses same procedure as WBC count except the dilution is?
NSS (Isotonic solution)
Differential cell count:
Cytocentrifugation requires addition of _______ to minimize cell distortion and increase the yield
1 drop of 30% albumin / 0.1 mL of CSF
Differential cell count:
A daily control slide for bacteria should also be prepared using:
_________ saline + ____ drops of ___ albumin
0.2 mL
2 drops
30% albumin
Type of Cell:
Lymphocytes and monocytes ratio:
Adults:_____
Children: _____
70/30
30/70
Type of cells:
Intracranial hemorrhage
a. Neutrophils
b. Erythrophages
c. Blast forms
d. Plasma cells
b
Type of cells:
Bacterial meningitis, cerebral hemorrhage
a. Neutrophils
b. Erythrophages
c. Blast forms
d. Plasma cells
a
Type of cells:
Multiple sclerosis, lymphocyte reactions
a. Neutrophils
b. Erythrophages
c. Blast forms
d. Plasma cells
d
Type of cells:
Leukemias and lymphomas
a. Neutrophils
b. Erythrophages
c. Blast forms
d. Plasma cells
c
Type of cells:
Diagnostic procedures
a. Ependymal choroidal cells
b. Malignant cells
c. Eosinophils
d. nRBCs
a
