4.1 - The microbial world Flashcards
microbiology
study of living things that are too small to be seen with unaided eye (microbes)
3 habitats that support majority of microbial diversity (3)
- soil
- fresh water
- seawater
microbe benefits (5)
- environment
- food
- bodily
- medical
- technology
microbe environmental benefits (2)
- decompose natural wastes and dead animal/plant matter
- produce nutrients for plants
microbe bodily benefits (2)
- gut flora
- regulates production of nutrients
Harmful effects of microbes?
human, animal and plant diseases
eukarya microorganisms (3)
- fungi
- protozoa
- algae
prokaryotic microorganisms (3)
- bacteria
- archaea
- viruses
features of bacterial taxonomy (4)
- shape
- metabolic activity
- pathogenicity
- nutritional requirements
key components of bacterial cell (6)
- rigid cell wall
- glycocalyx
- genetic material free floating in cytoplasm
- smaller rings of DNA called plasmids
- possesses ribosomes for protein synthesis
- flagella
role of rigid cell wall in bacteria (2)
- helps determine shape
- forms protective barrier
main component of bacteria cell wall
peptidoglycan
genetic material free floating in cytoplasm (bacteria)
found in single chromosome concentrated in a nucleoid
glycocalyx (3)
- sticky layer surrounding cell
- may form a capsule (organised and strongly attached)
- may form slime layer (disorganised and loosely attached)
plasmids (smaller rings of DNA in bacteria) (2)
- can self replicate
- contain non-essential genes that may confer advantage
gram negative bacteria (2)
- double plasma membrane separated by periplasm
- thin peptidoglycan layer covered by outer membrane, has porins and LPS (endotoxin)
gram positive bacteria (2)
- cell walls have thick outer peptidoglycan on outside of single plasma membrane
- reinforced by teichoic acids
main difference between gram positive and gram negative bacteria
positive - single plasma membrane
negative - double plasma membrane
gram staining
coloured purple. when combined with bacteria in sample stain will either stay purple inside bacteria (gram +ve) or turn pink (gram -ve)
chemotaxis
towards/away from chemical
phototaxis
towards/away from light
virulence
pathogens ability to cause damage/disease in host cell
structure of flagella
made from protein flagellin, anchored in inner membrane using basal body with the hook attaching flagellum to the motor protein
what drives rotation in the flagella
rotation of motor protein drives rotation of filament, propels the bacterium
DNA in archaea (3)
- free floating DNA, no organelles
- exists as rods, cocci and spirals
- reproduces by binary fission
how is the plasma membrane of archaea different?
- contains stabilising glycerol-ether linkages
- cell wall contains slightly different polymer (pseudopeptiglycan)
3 classification of fungi (nutrient absorption)
- saprophytes - dead organic matter
- biotrophs - living host tissue
- necrotrophs - kill host tissue and feed on it
Hyphae (2)
- long, thin filaments (effectively cells of fungus)
- grow and branch to form mycelium
septa
majority of fungi have septa dividing hyphae into compartments, usually have holes allowing movement of cytoplasm and sometimes organelles
woronin
protein lattices between each septum, can close them off in case of damage
2 methods of fungi reproduction (2)
- sexual (many fungi required to have different “mating types” analogous to male/female)
- asexual
essential structure for reproduction in fungi
microscopic fungal spore
protist
any eukaryotic organism that is not an animal, plant or fungus
ciliates (3)
- protists with tiny hair-like projections across surface of cell, allows them to move
- majority heterotrophs (consume other organisms, generally other protists or bacteria)
- commonly inhabit aquatic environments
ciliate nucleuses (2)
- tiny diploid micro nucleus - carries germline of cell
- large ampliploid macronucleus - takes care of general cell regulaation (generated from micronucleus by amplification/editing of genome)
ploid
refers to amount of complete sets of chromosomes in cell
symbiosis
any type of a close and long-term biological interaction between 2 biological organisms of different species (symbionts)
3 types of symbiosis (3)
- mutualistic
- commensalistic
- parasitic
mycorrhizae
- mutualistic symbiotic relationship between roots of most plant species and fungi
- bidirectional flow of nutrients between fungus/plants
lichens
symbioses between algae and fungi
legume
plants have root nodules containing bacterial species that fix nitrogen for them
do viruses contain cells/cell contents?
no
capsid (2)
- protein shell that encases a virus’s genetic material
- consists of nucleic acids and repeating protein units called capsomeres that makes up the protein coat
viral genomes (2)
- DNA or RNA based
- genetic material may be linear or circular
virus entry pathways (into cell) (2)
- membrane fusion
- receptor mediated endocytosis
- both result in release of viral genome into host cell
virus replication cycle (7)
- attachment and fusion
- entry and uncoating
- transcription
- translation
- assembly
- formation of virus envelope
- release