3.3 - nucleic acids Flashcards

1
Q

the central dogma

A

flow of genetic info from DNA -> RNA -> proteins

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2
Q

each nucleotide contains: (3)

A
  1. 5-carbon sugar (pentose)
  2. phosphate (1’-3’)
  3. base
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3
Q

2 types of nucleotide base: (2)

A
  1. pyrimidine (1 ring) - T/C
  2. purine (2 rings) - A/G
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4
Q

RNA nucleotide bases (4)

A
  1. guanine
  2. cytosine
  3. adenine
  4. uracil
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5
Q

DNA/RNA backbone

A

pentose sugar 3’-OH of one nucleotide covalently bonded via phosphodiester bond to adjacent phosphate group 5’-OH

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6
Q

what ends to nucleic acids have? (2)

A
  1. 5’
  2. 3’
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7
Q

difference between RNA and DNA sugars? (2)

A
  1. RNA - ribose sugar (2’-C of sugar linked to 2’-OH group)
  2. DNA - deoxyribose sugar (2’-C of sugar linked to H atom)
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8
Q

why is DNA much more stable than RNA?

A

absence of 2’-OH increases resistance to hydrolysis (deoxyribose sugar bonded to H atom not -OH)

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9
Q

what creates the DNA double helix? (2)

A
  1. sugar-phosphate backbone
  2. bases H-bonded together
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10
Q

orientation of 2 DNA strands (2)

A
  1. anti-parallel (opposite)
  2. 5’-3’ orientation opposite
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11
Q

what direction do DNA polymerase and RNA polymerase operate in?

A

5’-3’ direction

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12
Q

what non-covalent interactions stabilise the DNA double helix (excluding H-bonds) (2)

A
  1. van der waals
  2. hydrophobic effect (phosphate + sugar - hydrophilic/ base - hydrophobic)
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13
Q

RNA structure (2)

A
  1. single strand - can form 2x helix (RNA/RNA) and heteroduplex (RNA/DNA)
  2. can form other more complicated base-pair structures (due extra oxygen in ribose sugar compares to deoxyribose)
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14
Q

what feature of RNA supports the RNA world hypothesis? (2)

A
  1. dual capacity to act as catalyst and carrier of genetic info
  2. can catalyse chemical transformations using many same strategies as protein - based enzymes
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15
Q

catalytic RNA

A

ribozymes

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16
Q

ribozyme fundamental reactions (2)

A
  1. transesterification
  2. phosphodiester bond hydrolysis (cleavage)
17
Q

main types of RNA (2)

A
  1. informational - mRNA (encodes proteins)
  2. functional - tRNA, rRNA and others
18
Q

% of cell RNA made up by rRNA?

A

75%

19
Q

what encodes RNA?

A

DNA-based genes

20
Q

transfer RNA (2)

A
  1. involved in protein synthesis at ribosome and forms ‘clover leaf’ structure
  2. function as adaptor molecules - link amino acids for proteins with mRNA codons
21
Q

DNA at normal/elevated temps (3)

A
  1. double helix at normal temp
  2. strands disassociate at elevated temps
  3. re-anneal when temp lowered
22
Q

how can DNA strands disassociate/re-anneal?

A

H-bonds broken but phosphodiester bonds aren’t

23
Q

PCR - polymerase chain reaction (3)

A
  1. open DNA - denaturing
  2. primers find target - annealing (must know target DNA sequence)
  3. fill in complementary bases (complete copy) - DNA polymerase - extension
24
Q

organisation of genome: prokaryotes (2)

A
  1. each cell contains single circular genome, highly folded and compacted (supercoiled) to fit in small space
  2. may also have small circular plasmids, can replicate independently
25
Q

organisation of genome: eukaryotes (2)

A
  1. cells contain genome within nucleus, organised as linear double stranded chromosomes (homologous pair)
  2. mitochondria (and chloroplasts) contain own double stranded circular genome
26
Q

chromatin (2)

A
  1. eukaryotic DNA packaged together with histones -> nucleosomes
  2. chromatin further condensed into loops/coils, ultimately packaged into chromosomes
27
Q

what determines the accessibility of DNA to cellular machinery?

A

how tightly packed the nucleosome is