3.3 - nucleic acids Flashcards
the central dogma
flow of genetic info from DNA -> RNA -> proteins
each nucleotide contains: (3)
- 5-carbon sugar (pentose)
- phosphate (1’-3’)
- base
2 types of nucleotide base: (2)
- pyrimidine (1 ring) - T/C
- purine (2 rings) - A/G
RNA nucleotide bases (4)
- guanine
- cytosine
- adenine
- uracil
DNA/RNA backbone
pentose sugar 3’-OH of one nucleotide covalently bonded via phosphodiester bond to adjacent phosphate group 5’-OH
what ends to nucleic acids have? (2)
- 5’
- 3’
difference between RNA and DNA sugars? (2)
- RNA - ribose sugar (2’-C of sugar linked to 2’-OH group)
- DNA - deoxyribose sugar (2’-C of sugar linked to H atom)
why is DNA much more stable than RNA?
absence of 2’-OH increases resistance to hydrolysis (deoxyribose sugar bonded to H atom not -OH)
what creates the DNA double helix? (2)
- sugar-phosphate backbone
- bases H-bonded together
orientation of 2 DNA strands (2)
- anti-parallel (opposite)
- 5’-3’ orientation opposite
what direction do DNA polymerase and RNA polymerase operate in?
5’-3’ direction
what non-covalent interactions stabilise the DNA double helix (excluding H-bonds) (2)
- van der waals
- hydrophobic effect (phosphate + sugar - hydrophilic/ base - hydrophobic)
RNA structure (2)
- single strand - can form 2x helix (RNA/RNA) and heteroduplex (RNA/DNA)
- can form other more complicated base-pair structures (due extra oxygen in ribose sugar compares to deoxyribose)
what feature of RNA supports the RNA world hypothesis? (2)
- dual capacity to act as catalyst and carrier of genetic info
- can catalyse chemical transformations using many same strategies as protein - based enzymes
catalytic RNA
ribozymes