4- Importance and role of fine needle sampling (FNS)

Question 1

Define cytology

Answer 1

Study of cell number & type in a tissue mass/fluid accumulation to investigate its cause

Question 2

List 2 common cytological specimen sample

Answer 2

Fine needle samples
Touch imprints
Body fluids
Lavages

Question 3

Name the 2 techniques of taking an FNS/FNA

Answer 3

With no suction - used via capillary action (preferred method)

With suction (FNA)

Question 4

Name 2 things cytological examination may allow

Answer 4

Differentiation of inflammation from tissue growth
Differentiation of types of inflammation
Detect neoplasia
Differentiation of different fluids

Question 5

Advantages of FNS

Answer 5

sampling is quick, safe (can generally be done in conscious animal) and inexpensive

Question 6

List 2 limitations of FNS

Answer 6

False negatives - Failure to sample tumour tissue, poor exfoliation, extensive inflammation
False positives - Dysplasia can mimic neoplasia

Question 7

List some advantages and disadvantages of biopsy

Answer 7

AD= tissue architecture preserved, often more conclusive
Dis= more patient prep, more invasive, may need anaesthesia

Question 8

List the 3 neoplastic cell types (classification of tumour)

Answer 8

Epithelial, Round, Mesenchymal

Question 9

List 2 classifications of cystic lesions

Answer 9

Epidermal, sialocoele (Saliva), Serum, Haematoma

Question 10

T/F Epithelial neoplastic cells are associated with one another

Answer 10

True

Question 11

Which neoplastic cell type has a spindle shape?

Answer 11

Mesenchymal

Question 12

T/F Round neoplastic cells are adherent

Answer 12

False

Question 13

What information does histopathology provide

Answer 13

tissue architecture
adequacy of excision
invasion

Question 14

What is inflammatory dysplasia

Answer 14

proliferative changes and atypical morphology in some cells caused by inflammation/ irritation