3.b Instrumentation & Automation Flashcards

Beer-Lamber Law to Reflectance Spectrophotometer

1
Q

states that absorbance (optical density) is directly proportional to concentration

A= 2-log%T
A= abc

A

Beer-Lamber Law

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2
Q

Graph of Beer’s Law will show a straight line if:

Concentration vs Absorbance are plotted using

A. LINEAR PAPER
B. SEMILOG PAPER

A

A

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3
Q

Graph of Beer’s Law will show a straight line if:

Concentration vs Transmittance are plotted using

A. LINEAR PAPER
B. SEMILOG PAPER

A

B

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4
Q

Used to correct for interferences attributed from the sample:

A. Reagent Blank
B. Sample Blank

A

B

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5
Q

Interferences attributed from the sample

A

icteric, xanthrochromic

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6
Q

used to correct for interferences attributed from rgt

A. Reagent Blank
B. Sample Blank

A

A

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7
Q

Part of the spectrophotometer that isolates the specific wavelength of interest (desired wavelength):

A. MONOCHROMATOR
B. PHOTODETECTOR

A

A

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8
Q

All of the following are monochromator except?

A. Prisms
B. Colored Glass Filters
C. Barrier Layer Cell
D. Diffraction Gratings
E. Interference Filters

A

C

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9
Q

Part of spectrophotometer that converts light to electrical energy:

A. MONOCHROMATOR
B. PHOTODETECTOR

A

B

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10
Q

Which among the examples of Photodetector is the most sensitive?

A. barrier layer cell
B. photodiode
C. photomultiplier tube

A

C

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11
Q

Instrument with 2 photodetectors

A. DBSS
B. Fluorometer
C. FEP
D. AAS
E. Reflectance Spectrophotometer

A

A. DOUBLE BEAM IN SPACE SPECTROPHOTOMETRY

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12
Q

Instrument with 2 monochromators, positioned at 90deg:

A. DBSS
B. Fluorometer
C. FEP
D. AAS
E. Reflectance Spectrophotometer

A

B

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13
Q

Fluorometer

Characteristics of fluorescent light:

A. HIGH ENERGY, SHORT WAVELENGTH
B. LOW ENERGY, LONGER WAVELENGTH

A

B

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14
Q

Fluorometer

Characteristics of excitation light:

A. HIGH ENERGY, SHORT WAVELENGTH
B. LOW ENERGY, LONGER WAVELENGTH

A

A

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15
Q

Disadvantage of Fluorometry:

A

Quenching

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16
Q

Fluorometry

Effect of Quenching in fluorescence:

A

DECREASED FLUORESCENCE

17
Q

Instrument that measures the light/color emitted by the analyte; used for analytes that are easily excited by the flame

A. DBSS
B. Fluorometer
C. FEP
D. AAS
E. Reflectance Spectrophotometer

A

C. FLAME EMISSION PHOTOMETER

18
Q

FLAME EMISSION PHOTOMETER

Color emitted by Na =

A. YELLOW
B. VIOLET
C. RED

19
Q

FLAME EMISSION PHOTOMETER

Color emitted by K =

A. YELLOW
B. VIOLET
C. RED

20
Q

FLAME EMISSION PHOTOMETER

Color emitted by Li =

A. YELLOW
B. VIOLET
C. RED

21
Q

Dilution of serum used in Na/K analysis in FEP

A

1:100 or
1:200

22
Q

Modern FEP uses?

23
Q

This may be detected in spx;
used for Bipolar Disorder/Manic Depression

24
Q

Internal standards used in FEP

A. Li
B. Cs
C. Either
D. Neither
E. Both

A

E. Both LITHIUM, CESIUM

Note: Internal standards should not be present in the spx

25
Instrument used for analytes that are NOT EASILY excited by the flame; serves as reference method for CALCIUM and MAGNESIUM A. DBSS B. Fluorometer C. FEP D. AAS E. Reflectance Spectrophotometer
D. ATOMIC ABSORPTION SPECTROPHOTOMETER
26
ATOMIC ABSORPTION SPECTROPHOTOMETER Light source
HOLLOW CATHODE LAMP
27
ATOMIC ABSORPTION SPECTROPHOTOMETER Purpose of flame
ATOMIZER
28
ATOMIC ABSORPTION SPECTROPHOTOMETER After analyzing an analyte, the _ must be changed first, before proceeding with the analysis of the next analyte
hollow cathode lamp (light source coated w/ the element being measured)
29
Instrument that measures reflected light; serves as the principle behind Vitros, Kodak Ektachem and Automated Dipstick Readers: A. DBSS B. Fluorometer C. FEP D. AAS E. Reflectance Spectrophotometer
E. Reflectance Spectrophotometer
30
REFLECTANCE SPECTROPHOTOMETER Dry Slide Technology (layers; top to bottom) A. Spreader → Reagent → Scavenger → Support/Basal → Indicator B. Spreader → Scavenger → Reagent → Indicator → Support/Basal C. Spreader → Indicator → Reagent → Scavenger → Support/Basal D. Support/Basal → Indicator → Reagent → Scavenger → Spreader
B. Spreader → Scavenger → Reagent → Indicator → Support/Basal