3.b Instrumentation & Automation Flashcards
Beer-Lamber Law to Reflectance Spectrophotometer
states that absorbance (optical density) is directly proportional to concentration
A= 2-log%T
A= abc
Beer-Lamber Law
Graph of Beer’s Law will show a straight line if:
Concentration vs Absorbance are plotted using
A. LINEAR PAPER
B. SEMILOG PAPER
A
Graph of Beer’s Law will show a straight line if:
Concentration vs Transmittance are plotted using
A. LINEAR PAPER
B. SEMILOG PAPER
B
Used to correct for interferences attributed from the sample:
A. Reagent Blank
B. Sample Blank
B
Interferences attributed from the sample
icteric, xanthrochromic
used to correct for interferences attributed from rgt
A. Reagent Blank
B. Sample Blank
A
Part of the spectrophotometer that isolates the specific wavelength of interest (desired wavelength):
A. MONOCHROMATOR
B. PHOTODETECTOR
A
All of the following are monochromator except?
A. Prisms
B. Colored Glass Filters
C. Barrier Layer Cell
D. Diffraction Gratings
E. Interference Filters
C
Part of spectrophotometer that converts light to electrical energy:
A. MONOCHROMATOR
B. PHOTODETECTOR
B
Which among the examples of Photodetector is the most sensitive?
A. barrier layer cell
B. photodiode
C. photomultiplier tube
C
Instrument with 2 photodetectors
A. DBSS
B. Fluorometer
C. FEP
D. AAS
E. Reflectance Spectrophotometer
A. DOUBLE BEAM IN SPACE SPECTROPHOTOMETRY
Instrument with 2 monochromators, positioned at 90deg:
A. DBSS
B. Fluorometer
C. FEP
D. AAS
E. Reflectance Spectrophotometer
B
Fluorometer
Characteristics of fluorescent light:
A. HIGH ENERGY, SHORT WAVELENGTH
B. LOW ENERGY, LONGER WAVELENGTH
B
Fluorometer
Characteristics of excitation light:
A. HIGH ENERGY, SHORT WAVELENGTH
B. LOW ENERGY, LONGER WAVELENGTH
A
Disadvantage of Fluorometry:
Quenching
Fluorometry
Effect of Quenching in fluorescence:
DECREASED FLUORESCENCE
Instrument that measures the light/color emitted by the analyte; used for analytes that are easily excited by the flame
A. DBSS
B. Fluorometer
C. FEP
D. AAS
E. Reflectance Spectrophotometer
C. FLAME EMISSION PHOTOMETER
FLAME EMISSION PHOTOMETER
Color emitted by Na =
A. YELLOW
B. VIOLET
C. RED
A
FLAME EMISSION PHOTOMETER
Color emitted by K =
A. YELLOW
B. VIOLET
C. RED
B
FLAME EMISSION PHOTOMETER
Color emitted by Li =
A. YELLOW
B. VIOLET
C. RED
C
Dilution of serum used in Na/K analysis in FEP
1:100 or
1:200
Modern FEP uses?
Cesium
This may be detected in spx;
used for Bipolar Disorder/Manic Depression
Li
Internal standards used in FEP
A. Li
B. Cs
C. Either
D. Neither
E. Both
E. Both LITHIUM, CESIUM
Note: Internal standards should not be present in the spx
Instrument used for analytes that are NOT EASILY excited by the flame; serves as reference method for CALCIUM and MAGNESIUM
A. DBSS
B. Fluorometer
C. FEP
D. AAS
E. Reflectance Spectrophotometer
D. ATOMIC ABSORPTION SPECTROPHOTOMETER
ATOMIC ABSORPTION SPECTROPHOTOMETER
Light source
HOLLOW CATHODE LAMP
ATOMIC ABSORPTION SPECTROPHOTOMETER
Purpose of flame
ATOMIZER
ATOMIC ABSORPTION SPECTROPHOTOMETER
After analyzing an analyte, the _ must be changed first, before proceeding with the analysis of the next analyte
hollow cathode lamp (light source coated w/ the element being measured)
Instrument that measures reflected light; serves as the principle behind Vitros, Kodak Ektachem and Automated Dipstick Readers:
A. DBSS
B. Fluorometer
C. FEP
D. AAS
E. Reflectance Spectrophotometer
E. Reflectance Spectrophotometer
REFLECTANCE
SPECTROPHOTOMETER
Dry Slide Technology (layers; top to bottom)
A. Spreader → Reagent → Scavenger → Support/Basal → Indicator
B. Spreader → Scavenger → Reagent → Indicator → Support/Basal
C. Spreader → Indicator → Reagent → Scavenger → Support/Basal
D. Support/Basal → Indicator → Reagent → Scavenger → Spreader
B. Spreader → Scavenger → Reagent → Indicator → Support/Basal
