2. Specimen Collection Flashcards
_ is usually taken early in the morning, before the patient has eaten or before patient becomes physically active
BASAL STATE
Number of fasting hours is part of:
PATIENT PREPARATION
Fasting for Glucose
8-10 hrs of overnight fasting
Fasting for Lipids (TAG)
atleast 12 hrs
T or F
cholesterol does not require fasting
T
Puncture site is disinfected/cleansed using
ISOPROPYL ALCOHOL
Antiseptic used in ethanol testing; also a class of QUATS
BENZALKONIUM CHLORIDE
Photosensitive analytes
BILIRUBIN, CK
What happens to Bili when spx is exposed to light?
False increase
Analytes that require chilling of specimen with ice
A. Ammonia
B. Blood Gas
C. Both
D. Neither
C
ANALYTES WITH DIURNAL VARIATION
INCREASED IN AM:
ACTH
cortisol
iron
Aldosterone
ANALYTES WITH DIURNAL VARIATION
INCREASED IN PM:
ACP
GH
PTH
TSH
Which of the following are sources of error in ammonia analysis?
A) Smoking
B) Detergent
C) Temperature fluctuations
D) Both A and B
D
Patient A has a history of tobacco smoking. What results are expected to be INCREASED?
i. Hb concentration
ii. Eosinophils
iii. Hct
iv. IgA, IgG, IgM
A) i and ii
B) i and iii
C) ii and iv
D) iii and iv
B
Others: plasma catecholamines, serum cortisol, plasma fatty free acids lactate, insulin, epinephrine, growth hormone, 5-HIAA (in urine)
leukocyte (WBC) count; neutrophils; monocytes
blood carboxyhemoglobin levels, erythrocyte (RBC) count, MCV, IgE
Patient A has a history of tobacco smoking. What results are expected to be DECREASED?
i. Hb concentration
ii. Eosinophils
iii. Hct
iv. IgA, IgG, IgM
A) i and ii
B) i and iii
C) ii and iv
D) iii and iv
C
Others: Vitamin B12
decreased sperm counts and motility
Patient B presents with a long history of alcohol use. The doctor orders several lab tests to assess the patient’s liver function and overall health. Based on the patient’s history, which of the following analytes would you expect to be increased?
A. Glucose, albumin, transferrin
B. Aminotransferases, lipoproteins, bilirubin, ketone bodies, triglycerides
B
Patient B presents with a long history of alcohol use. The doctor orders several lab tests to assess the patient’s liver function and overall health. Based on the patient’s history, which of the following analytes would you expect to be decreased?
A. Glucose, albumin, transferrin
B. Aminotransferases, lipoproteins, bilirubin, ketone bodies, triglycerides
A
Effect of icteric and hemolyzed sample in ALP determinations:
INCREASED ALP
Icteric = bilirubin
Hemolyzed = hgb
Analyte detects to assess completeness of a 24-hour urine:
CREATININE
Specimen used in Newborn Screening:
CAPILLARY BLOOD (BLOOD SPOT)
Heparinized plasma
A. Preferred sample for electrolyte analysis
B. Preferred specimen for ammonia analysis
C. Most common sample for electrolyte analysis
A
Serum = Most common sample for electrolyte analysis
EDTA PLASMA/HEPARINIZED PLASMA
A. Preferred sample for electrolyte analysis
B. Preferred specimen for ammonia analysis
B
Ammonia is produced during?
Clotting
Vacutainer tubes that contains EDTA:
LAVENDER, PINK, WHITE, TAN
Specimen for ACP determination:
SERUM, VAGINAL WASHING
T or F
There is no effect of using non-anticoagulated tube for ACP
T
Effect of direct rectal exam in ACP levels is increased. Why?
Stimulation of the prostate (Physiologic)
T or F
Effect of intramuscular injection in CK-MM levels: DECREASED
F
Effect of intramuscular injection in CK-MM levels: INCREASED
Effect of breast examination on prolactin levels is increased. Why?
Stimulation of the Pituitary gland
Bilirubin Level of Icteric sample
A. 25mg/L
B. 430 mmol/L
C. Both
D. Neither
C
Bilirubin Level associated with jaundice:
2-3mg/dL
this is the accumulation of bilirubin in the brain; danger is at certainty at levels exceeding 20mg/dL
Kernicterus
yellowish discoloration of skin and sclera of eyes
Jaundice
Patient C presents with elevated bilirubin levels of 25 mg/L (430 mmol/L) due to liver dysfunction. The laboratory performs several tests to assess the patient’s overall health, but the high bilirubin levels may interfere with certain assays. Based on the patient’s bilirubin levels, which of the following laboratory tests are likely to be affected?
A) Albumin (HABA method)
B) Cholesterol (using ferric chloride reagent)
C) Total protein (using Biuret reaction)
D) All of the above
D
Fasting requirement for Lipid analysis:
AT LEAST 12 HOURS
Lipemia occurs when TAG level exceeds _. It causes interference for amylase, urates, urea, creatine kinase, bilirubin and total protein.
400 mg/dL
this kind of sample might indicate that the patient is in the non-fasting state
Lipemia
Blood volume of an average male:
5-7L
Blood volume of an average female:
4-6L
Preferred vein for venipuncture:
Median Cubital Vein
median>cephalic>basilic
Analytical Testing performed outside the laboratory is known as:
Point of Care Testing
