2. Specimen Collection

Question 1

_ is usually taken early in the morning, before the patient has eaten or before patient becomes physically active

Answer 1

BASAL STATE

Question 2

Number of fasting hours is part of:

Answer 2

PATIENT PREPARATION

Question 3

Fasting for Glucose

Answer 3

8-10 hrs of overnight fasting

Question 4

Fasting for Lipids (TAG)

Answer 4

atleast 12 hrs

Question 5

T or F

cholesterol does not require fasting

Answer 5

T

Question 6

Puncture site is disinfected/cleansed using

Answer 6

ISOPROPYL ALCOHOL

Question 7

Antiseptic used in ethanol testing; also a class of QUATS

Answer 7

BENZALKONIUM CHLORIDE

Question 8

Photosensitive analytes

Answer 8

BILIRUBIN, CK

Question 9

What happens to Bili when spx is exposed to light?

Answer 9

False increase

Question 10

Analytes that require chilling of specimen with ice

A. Ammonia
B. Blood Gas
C. Both
D. Neither

Answer 10

C

Question 11

ANALYTES WITH DIURNAL VARIATION

INCREASED IN AM:

Answer 11

ACTH
cortisol
iron
Aldosterone

Question 12

ANALYTES WITH DIURNAL VARIATION

INCREASED IN PM:

Answer 12

ACP
GH
PTH
TSH

Question 13

Which of the following are sources of error in ammonia analysis?

A) Smoking
B) Detergent
C) Temperature fluctuations
D) Both A and B

Answer 13

D

Question 14

Patient A has a history of tobacco smoking. What results are expected to be INCREASED?

i. Hb concentration
ii. Eosinophils
iii. Hct
iv. IgA, IgG, IgM

A) i and ii
B) i and iii
C) ii and iv
D) iii and iv

Answer 14

B

Others: plasma catecholamines, serum cortisol, plasma fatty free acids lactate, insulin, epinephrine, growth hormone, 5-HIAA (in urine)
leukocyte (WBC) count; neutrophils; monocytes
blood carboxyhemoglobin levels, erythrocyte (RBC) count, MCV, IgE

Question 15

Patient A has a history of tobacco smoking. What results are expected to be DECREASED?

i. Hb concentration
ii. Eosinophils
iii. Hct
iv. IgA, IgG, IgM

A) i and ii
B) i and iii
C) ii and iv
D) iii and iv

Answer 15

C

Others: Vitamin B12
decreased sperm counts and motility

Question 16

Patient B presents with a long history of alcohol use. The doctor orders several lab tests to assess the patient’s liver function and overall health. Based on the patient’s history, which of the following analytes would you expect to be increased?

A. Glucose, albumin, transferrin
B. Aminotransferases, lipoproteins, bilirubin, ketone bodies, triglycerides

Answer 16

B

Question 17

Patient B presents with a long history of alcohol use. The doctor orders several lab tests to assess the patient’s liver function and overall health. Based on the patient’s history, which of the following analytes would you expect to be decreased?

A. Glucose, albumin, transferrin
B. Aminotransferases, lipoproteins, bilirubin, ketone bodies, triglycerides

Answer 17

A

Question 18

Effect of icteric and hemolyzed sample in ALP determinations:

Answer 18

INCREASED ALP

Icteric = bilirubin
Hemolyzed = hgb

Question 19

Analyte detects to assess completeness of a 24-hour urine:

Answer 19

CREATININE

Question 20

Specimen used in Newborn Screening:

Answer 20

CAPILLARY BLOOD (BLOOD SPOT)

Question 21

Heparinized plasma

A. Preferred sample for electrolyte analysis
B. Preferred specimen for ammonia analysis
C. Most common sample for electrolyte analysis

Answer 21

A

Serum = Most common sample for electrolyte analysis

Question 22

EDTA PLASMA/HEPARINIZED PLASMA

A. Preferred sample for electrolyte analysis
B. Preferred specimen for ammonia analysis

Answer 22

B

Question 23

Ammonia is produced during?

Answer 23

Clotting

Question 24

Vacutainer tubes that contains EDTA:

Answer 24

LAVENDER, PINK, WHITE, TAN

Question 25

Specimen for ACP determination:

Answer 25

SERUM, VAGINAL WASHING

Question 26

T or F There is no effect of using non-anticoagulated tube for ACP

Answer 26

T

Question 27

Effect of direct rectal exam in ACP levels is increased. Why?

Answer 27

Stimulation of the prostate (Physiologic)

Question 28

T or F Effect of intramuscular injection in CK-MM levels: DECREASED

Answer 28

F Effect of intramuscular injection in CK-MM levels: INCREASED

Question 29

Effect of breast examination on prolactin levels is increased. Why?

Answer 29

Stimulation of the Pituitary gland

Question 30

Bilirubin Level of Icteric sample A. 25mg/L B. 430 mmol/L C. Both D. Neither

Answer 30

C

Question 31

Bilirubin Level associated with jaundice:

Answer 31

2-3mg/dL

Question 32

this is the accumulation of bilirubin in the brain; danger is at certainty at levels exceeding 20mg/dL

Answer 32

Kernicterus

Question 33

yellowish discoloration of skin and sclera of eyes

Answer 33

Jaundice

Question 34

Patient C presents with elevated bilirubin levels of 25 mg/L (430 mmol/L) due to liver dysfunction. The laboratory performs several tests to assess the patient's overall health, but the high bilirubin levels may interfere with certain assays. Based on the patient's bilirubin levels, which of the following laboratory tests are likely to be affected? A) Albumin (HABA method) B) Cholesterol (using ferric chloride reagent) C) Total protein (using Biuret reaction) D) All of the above

Answer 34

D

Question 35

Fasting requirement for Lipid analysis:

Answer 35

AT LEAST 12 HOURS

Question 36

Lipemia occurs when TAG level exceeds _. It causes interference for amylase, urates, urea, creatine kinase, bilirubin and total protein.

Answer 36

400 mg/dL

Question 37

this kind of sample might indicate that the patient is in the non-fasting state

Answer 37

Lipemia

Question 38

Blood volume of an average male:

Answer 38

5-7L

Question 39

Blood volume of an average female:

Answer 39

4-6L

Question 40

Preferred vein for venipuncture:

Answer 40

Median Cubital Vein median>cephalic>basilic

Question 41

Analytical Testing performed outside the laboratory is known as:

Answer 41

Point of Care Testing