3.a Instrumentation & Automation Flashcards
Centrifuge to Color & Energy
Centrifuge type recommended for Serum Separator Tubes (SST)
HORIZONTAL
HEAD CENTRIFUGE/ SWINGING BUCKET TYPE OF CENTRIFUGE
Disinfection of centrifuge is done:
EVERY WEEK
Calibration for speed of centrifuge is done every:
3 MONTHS (QUARTERLY)
Device used to calibrate the speed of centrifuge:
TACHOMETER/STROBE LIGHT
Pipet with the highest precision and accuracy:
VOLUMETRIC PIPET
OSTWALD FOLIN PIPET
BLOW OUT PIPET
MOHR PIPET
VOLUMETRIC PIPET
Pipets without graduations lines at the tip:
VOLUMETRIC PIPET
OSTWALD FOLIN PIPET
BLOW OUT PIPET
MOHR PIPET
MOHR PIPET
Transfer pipet used for viscous solutions:
VOLUMETRIC PIPET
OSTWALD FOLIN PIPET
BLOW OUT PIPET
MOHR PIPET
OSTWALD FOLIN PIPET
Pipets with etched rings or continuous rings:
VOLUMETRIC PIPET
OSTWALD FOLIN PIPET
BLOW OUT PIPET
MOHR PIPET
BLOW OUT PIPET
Design & Drainage Characteristic
To deliver; self-draining (2)
VOLUMETRIC PIPET
OSTWALD FOLIN PIPET
SEROLOGIC
MOHR PIPET
VOLUMETRIC PIPET
MOHR PIPET
Design & Drainage Characteristic
To deliver; blow-out (2)
VOLUMETRIC PIPET
OSTWALD FOLIN PIPET
SEROLOGIC
MOHR PIPET
OSTWALD FOLIN PIPET
SEROLOGIC
holds but does not dispense the exact same value
A. TC
B. TD
A
delivers the exact volume it holds
A. TC
B. TD
B
Cleaning solution for glassware; is made up of K dichromate + Sulfuric acid/Nitric acid
ACID DICHROMATE
Sterilization technique for glassware:
DRY HEAT OVEN TECHNIQUE
True about DRY HEAT OVEN TECHNIQUE
160-180C
1.5-3 hrs
Bacillus subtilis van niger
A. Only 1 is correct
B. 2 are correct
C. All are correct
D. All are incorrect
B
160-180C
1.5-2 hrs
Bacillus subtilis van niger
used in Gunthrie test for PKU
Bacillus subtilis van niger
PKU odor
mousy/ musty urine odor
A _ operates by moving the piston in the pipet tip or
barrel, much like a hypodermic needle.
It does not require a different tip for
each use.
Because of carry over concerns, rinsing and blotting between samples
may be required.
positive displacement pipet
Analyzer characterized by continuous system of tubing:
CONTINUOUS FLOW
ANALYZER
Primary source of error in continuous flow analyzer:
A. carry over
B. air bubbles
A
Reactants in the continuous tubing are separated using:
AIR BUBBLES
Purpose of coiled tubings in continuous flow analyzer
FOR MIXING PURPOSES
Which of the following is/are an example of a continuous flow analyzer?
A) AutoAnalyzer
B) Technicon
C) Both A and B
D) Spectrophotometer
C
Analyzer characterized by spinning rotor:
A. CONTINUOUS FLOW
ANALYZER
B. CENTRIFUGAL ANALYZER
C. DISCRETE ANALYZER
D. NOTA
B
Most popular type of analyzer; analyzer type that can perform random access,
batch and sequential analysis:
A. CONTINUOUS FLOW
ANALYZER
B. CENTRIFUGAL ANALYZER
C. DISCRETE ANALYZER
D. NOTA
C
Examples of Discrete Analyzers (2)
VITROS (Ortho-Clinical D.)
ADVIA & DIMENSION (Siemens)
Others:
ACA (Dupont)
ASTRA/SYNCHRON (Beckman)
CENTRIFICHEM (Baker) COULTER CHEMISTRY (Coulter Electronics)
KDA (American Monitor) ARCHITECT and TDX (Abbott)
PARAMAX
HITACHI (Boehringer Mannheim/Roche)
is the unit of measure for the wavelength of radiant energy (light).
NANOMETER
T or F
Wavelength is directly proportional to the amount of energy. The longer the wavelength, the lower the energy; the shorter the wavelength, the higher the energy.
F
inversely
Spectra of Light
Visible Light Spectrum
400-700nm
4nm – 400nm
700nm – 0.3cm
400-700nm
Spectra of Light
UV light
400-700nm
4nm – 400nm
700nm – 0.3cm
4nm – 400nm
Spectra of Light
IR light
400-700nm
4nm – 400nm
700nm – 0.3cm
700nm – 0.3cm
Color with the highest energy
VIOLET
Color with the longest wavelength
RED