3.3 Organisms exchange substances with their environment Flashcards

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1
Q

What are xerophytes?

A

-plants living in hot, dry environments, adapted to minimise water loss
-e.g. cacti

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2
Q

Why do xerophytes need to minimise water loss?

A

hot environment, and low water vapour in the air
-the risk for losing water through transpiration is very high in xerophytes

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3
Q

Adaptations of xerophytes for the opposing needs for efficient gas exchange and the limitation of water loss

A

Sunken stomata:
➤traps moist air
➤reduces water potential gradient
➤hence lowers water loss in leaves

Less stomata:
➤reduces water loss
➤respond to low water availability by closing stomata to prevent further water loss

Thick waxy cuticle:
➤less water evaporates from leaf surface

Shiny cuticle:
➤reflects sunlight away
➤less heat absorbed
➤prevents water loss through transpiration

Thick stems:
➤stores water
➤less is lost

Large root systems:
➤maximises amount of water taken up from ground

Pointed spines:
➤often instead of leaves
➤less surface area available for water loss

Rolled leaves:
➤traps a layer of humid air

Densely packed mesophyll:
➤reduces surface area for water loss
➤larger surface area for photosynthesis

Trichomes (hairs):
➤fine, so can trap moist air
➤reduces water potential

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4
Q

Why do organisms need to exchange materials with their environment?

A
  1. Cells need oxygen and nutrients for metabolism.
  2. Waste products of metabolism e.g. CO2 and urea need to be excreted from the body.
  3. To maintain body temperature of animals (exchange of heat between the body and the environment)
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5
Q

Why is exchange easier for single-celled organisms?

A

Shorter diffusion pathway

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6
Q

Ways to increase SA:V ratio?

A

-folds in surface of the cell membrane increase surface area
-developing into long, thin, or elongated shaped cells
-presence of large vacuoles inside the cell
╰┈➤ pushes other organelles to the side of the cell
╰┈➤ easier diffusion of materials

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7
Q

2 main factors influencing heat exchange?

A
  1. Size
    -smaller surface area to volume ratio makes heat loss from the body harder
    -but, small organisms have higher surface area to volume ratio
    -hence, these organisms need higher metabolic rate to generate more heat for it to stay warm.
  2. Shape
    -difference in shape = difference in their surface area
    -animals with more compact shape have small surface area
    -hence high metabolic rate not needed to minimize heat loss from the body
    -but animals with less compact shape have higher surface area
    -hence high metabolic rate needed to produce more heat to maintain body temperature
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8
Q

Why can’t Animals/Plants perform exchange via their surface?

A

-have a small surface area to volume ratio

-multicellular (large diffusion distance and high demand)

-impermeable surface (prevent pathogens entering and reduce water loss)

-therefore, require specialised Exchange & Transport systems

-exchange system = increases rate of diffusion of nutrients in and wastes out

-transport system = deliver nutrients and remove waste from all cells

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9
Q

What does the human gas exchange system consist of?
What is the function of lungs?
Structure of trachea/bronchi?
Structure of alveoli?
Structure of bronchioles?

A

Lungs are the site of gas exchange in mammals
╰┈➤ oxygen into blood – used in cells for respiration
╰┈➤ carbon dioxide out of the blood – toxic waste product of respiration

Cartilage:
-involved in supporting the trachea and bronchi
-prevents lungs from collapsing in the event of pressure drop during exhalation.

Ciliated epithelium:
-present in bronchi, bronchioles and trachea
-involved in moving mucus along to prevent lung infection by moving it towards the throat where it can be swallowed

Goblet cel:
-cells present in the trachea, bronchi and bronchioles involved in mucus secretion
-traps bacteria and dust
-reduces risk of infection with using lysosomes digest bacteria.

Smooth muscle:
-ability to contract enables them to constrict the airway
-hence controls its diameter and the flow of air to and from the alveoli.

Elastic fibres:
-stretch when we exhale
-recoil when we inhale
-hence controls the flow of air.

Structure of trachea/bronchi:
● The lungs contain a number of parts that facilitate the gas exchange.
╰┈➤Trachea - It functions to funnel the inhaled air into the lungs, while also facilitating the removal of inhaled air out of the lungs.
╰┈➤Bronchi - These are smaller passages where air enters the lungs from the trachea
╰┈➤Bronchioles - The bronchi further divide into smaller, and smaller passages called bronchioles.

Alveoli
-contain thin-walled cells that are directly connected to capillaries
-oxygen transferred down a concentration gradient from the alveoli into blood cells
-at the same time, carbon dioxide is transferred from the blood cells to the alveoli
-wall made of c-shaped cartilage
-cartilage is strong so trachea/bronchi do not collapse
-cartilage is c-shaped to give flexibility
-lining made of goblet cells and ciliated epithelial cells
-goblet cells make mucus, which traps pathogens/particles
-ciliated epithelial cells have cilia, which pushes mucus up and out of lungs

Structure of bronchioles?
-wall made of smooth muscle
-smooth muscle contracts, lumen narrows, bronchiole constricts
-occurs when surrounded by noxious gases – reduces amount reaching alveoli
-lining made of goblet cells and ciliated epithelial cells

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10
Q

The essential features of the alveolar epithelium as a surface over which gas exchange takes place

A

-millions of tiny alveoli that are folded (large surface area)
-thin wall/one cell thick/squamous epithelial cells (short diffusion distance)
-elastic tissue in wall (stretches when breathing in to increase surface area, recoils when breathing out to push the air out)
-ventilation maintains concentration gradient (high oxygen, low carbon dioxide)

How O2 moves from the alveoli to the capillaries? by simple diffusion passes through the alveolar epithelium and capillary epithelium

How CO2 moves from capillaries to the alveoli?
-by simple diffusion
-passes through the capillary epithelium and alveoli epithelium

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11
Q

Describe the process of ventilation

State the formula for pulmonary ventilation

A

Breathing In/Inhalation:
-internal intercostalmuscles relax
-external intercostal muscles contract
-rib cage moves up and out
-diaphragm contracts (flattens)
-increase in volume in thoracic cavity
-decrease in pressure
-so air moves in

Breathing Out/Exhalation
-internal intercostal musclescontract
-external intercostal muscles relax
-rib cage moves down and in
-diaphragm relaxes (back to dome shape)
-decrease in volume in thoracic cavity
-increase in pressure
-so air pushed out (aided by elastic recoil in the alveoli)

Formula for Pulmonary Ventilation
PV = tidal volume x ventilation rate
tidal volume = volume of air breathed in/out in one breath
ventilation rate = number of breaths per minute

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12
Q

Why can microorganisms exchange substances via their surface

A

-have a large surface area to volume ratio
-have a short diffusion distance
-have low demand

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13
Q

How is a single-celled organism adapted for gaseous exchange across its body surface

A

Thin cell membrane:
-shorter diffusion distance
-hence faster rate of O2 in and CO2 out

Larger surface area to volume ratio:
-faster rate of diffusion due to more space for the exchange of materials

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14
Q

How is the tracheal system of an insect adapted for gaseous exchange across its body surface

A
  1. Air move into trachea through pores on surface called spiracles
  2. oxygen travels down the concentration gradient towards the respiring cells
  3. the tracheae branch off into smaller tracheoles
    ╰┈➤have a thin, permeable wall
    ╰┈➤ go to individual cells
    ╰┈➤means that oxygen diffuses directly into the respiring cells
  4. CO2 diffuses from the cells
    ╰┈➤ moving down its conc gradient into tracheal tubes towards spiracles and released into the atmosphere.
  5. insects use rhythmic abdominal movements to move air in and out of spiracles

Structure of tracheal system:
-starts with openings on body surface called spiracles
-spiracles contain valves, open = gas exchange, closed = prevent water loss
-spiracles connect to trachea
-trachea connect to tracheoles
-tracheoles connect directly to respiring cells (delivering oxygen, removing carbon dioxide)

Why insects need a tracheal system:
-multicellular organism so has a small surface area to volume ratio, large diffusion distance, high demand & body surface made of exoskeleton (impermeable barrier to reduce water loss)
-therefore, cannot perform gas exchange (O2 in/CO2 out) via their surface, they require a tracheal system

at rest = down a concentration gradient, oxygen moves in & carbon dioxide moves out by simple diffusion
when active = by ventilation, air inhaled for mass flow of O2 in & air exhaled for mass flow of CO2 out

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15
Q

How are the gills of a fish adapted for gaseous exchange across its body surface

A

–multicellular organism so has a small surface area to volume ratio, large diffusion distance, high demand & body surface impermeable
-also, as fish live underwater, it is harder to obtain oxygen from the environment due to lower oxygen concentration in water than in air
-therefore, cannot perform gas exchange (O2 in/CO2 out) via their surface, they require gills

-gills
-made up of thin plates called gill filaments
-provides large surface area for gaseous exchange

-lamellae
-tiny structures with lots of blood capillaries and thin surface layer of cells
-speeds up diffusion, as shorter diffusion distance
- covers gill filaments which increases the surface area further

-countercurrent flow
-blood and water flow opposite
-ventilation brings in pure water (high oxygen, low carbon dioxide)
- circulation brings in deoxygenated blood (low oxygen, high carbon dioxide)
-diffusion gradient for oxygen uptake is maintained across entire width of gill lamellae
- equilibrium is never reached, hence transfer of oxygen is more efficient
-majority of oxygen available in the water is absorbed into the blood

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16
Q

How are the leaves of dicotyledonous plants adapted for gaseous exchange across its body surface

A

-stomata open to allow gas exchange and close if the plant is losing too much water
-guard cells control the opening and closing of stomata.

Mesophyll cells are differentiated into palisade and spongy mesophyll:
-Palisade Mesophyll: Elongated cells beneath the upper epidermis, densely packed with chloroplasts for optimal light absorption.
-Spongy Mesophyll: Loosely arranged cells with intercellular spaces beneath the palisade layer, facilitating gas movement and exchange.

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17
Q

Outline some lung diseases and risk factors/symptoms associated with it

A

General causes for lung diseases:
-genetic makeup
-occupation
-air pollution
-tobacco from smoking
(remember GOAT)

Pulmonary fibrosis:
-epithelial tissue in lungs become irreversibly thicker and less elastic
-thicker = longer diffusion distance
-less elastic = cannot recoil or force air out of alveoli
-hence results in shortness of breath and chest pain

Lung cancer:
-if malignant, it can split and spread into capillaries and organs
-caused by uncontrollable dividing of cells via mitosis

Chronic Obstructive Pulmonary Disease (COPD):
-includes emphysema and chronic bronchitis:
-emphysema = smoking destroys alveoli (either elastin is broken down or alveoli fuses together, reducing surface area to volume ratio)
-chronic bronchitis = more mucus produced due to destroyed cilia, and the irritation leads to scar tissue

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18
Q

What is the importance of digestion?
Function of intestines?

A

Large biological molecules must be hydrolysed to smaller molecules so they can be absorbed across cell membranes

Site of exchange of digested nutrients in mammals
Small intestine absorbs small soluble nutrients, e.g. glucose, amino acids
Large intestine absorbs water

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19
Q

Outline the digestion and absorption of carbohydrates, including any enzymes

A

Enzymes:
Starch/glycogen —> maltose:
-salivary amylase in mouth
-pancreatic amylase in small intestine
Maltose —> glucose:
-maltase on lining of small Intestine
Lactose —> glucose and galactose:
-lactase on lining of small intestine
Sucrose —> glucose and fructose:
-sucrase on lining of small intestine

Digestion:
-in the mouth, starch is digested into shorter polysaccharides by salivary amylase (partial digestion as some maltose appears)
-food travels to stomach via oesophagus
-no digestion in stomach due to lack of necessary enzymes
-salivary amylase that comes to stomach with food is unable to work due to stomach pH being too low (due to HCl)
-in the duodenum, complete digestion of starch to maltose occurs with pancreatic amylase
-disaccharidase in lining of ileum digests all carbohydrates to monosaccharides

Absorption of glucose in small intestine
-occurs via co-transport mechanism
-sodium ions are actively transported from the cells lining the ileum into the blood
-ATP hydrolysed to ADP and Pi
-energy released allows ions to be moved against concentration gradient
-lowers the sodium ion concentration in the cell
-hence sodium ions move from the lumen of ileum into the cell
-this pulls in glucose via a co-transporter protein
-hence glucose builds up in the cell and moves into the blood by facilitated diffusion

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20
Q

Outline the digestion and absorption of lipids, including any enzymes

A

Digestion:
-bile breaks down triglycerides before lipase (by emulsification)
-smaller droplets of lipid produced have larger surface area for enzyme action
-hence faster hydrolysis
-bile salt has two parts
-hydrophobic part attaches to broken triglyceride
-hydrophilic part sticks to outside to prevent broken lipids from rejoining
-ester bonds are broken by lipase (found in pancreas)
-inside lumen of duodenum
-triglyceride (in micelle form) hydrolysed to glycerol, fatty acid and monoglyceride

Absorption:
-micelles bring products to ileum from duodenum
-products are still attached to bile salt
-micelles release products on microvilli on the surface of lining cell
-as they are non-polar/hydrophobic, can pass through phospholipids of membrane by simple diffusion
-glycerol, fatty acid, monoglyceride enter smooth endoplasmic reticulum by simple diffusion
-in SER, they undergo re-esterification and form triglyceride again
-triglyceride moves from SER to Golgi apparatus
-protein added to triglyceride to form lipoprotein
-lipoprotein released from lining cell into lymph vessel by exocytosis
-lining cell of blood capillary produces lipase which hydrolyses lipoprotein into fatty acid/glycerol
-lymph vessel opens into bloodstream
-products diffuses into body cells

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21
Q

Outline the digestion of proteins, including any enzymes

A

-endopeptidase: breaks polypeptide from middle of chain
-two types of endopeptidase = pepsin (found in stomach) and trypsin (found in pancreas)
-exopeptidase: breaks polypeptide from a terminal (found in pancreas)
-two types of exopeptidase = aminopeptidase/carboxypeptidase
-endopeptidase produces shorter polypeptide chains
-exopeptidase produces amino acids
-dipeptidase (found in lining cell of ileum alongside disaccharidase) hydrolyses peptide bond in dipeptides

Digestion:
-pepsin hydrolyses proteins to smaller polypeptides in stomach
-partially digested protein enters duodenum, which receives pancreatic juice
-pancreatic juice contains trypsin, which further reacts on partially digested protein
-sodium hydrogencarbonate in pancreatic juice and bile (hence pH is around 7-8)
-from pancreatic juice, exopeptidase produces a mixture of amino acids and some dipeptides
-when products are absorbed into ileum, dipeptides are further digested into amino acids by dipeptidase in the membrane of lining cell

Absorption:
-absorbed similarly to glucose: by co-transport of Na+ ions
-sodium ions are actively transported from the cells lining the ileum into the blood
-lowers the sodium ion concentration in the cell
-hence sodium ions move from the lumen of ileum into the cell
-this pulls in amino acids via a co-transporter protein
-hence amino acids builds up in the cell and moves into the blood by facilitated diffusion
-amino acids are then absorbed into blood

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22
Q

Adaptations of ileum for digestion/absorption?

A

-folded villi to increase surface area of wall of ileum
-lining cells have microvilli (folded membranes) to further increase surface area
-increased folding also means larger amounts of disaccharidase and dipeptidase can be stored in membrane of lining cell of ileum
-allows faster rate of hydrolysis, as more enzymes available to form E-S complexes
-many mitochondria in lining cells, as co-transport is active process
-hence energy is required for absorption of glucose/amino acids

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23
Q

Describe the processes involved in the absorption and transport of digested lipid molecules from the ileum into lymph vessels. (5 marks)

A
  1. Micelles contain bile salts and fatty acids/monoglycerides;
    Ignore other correct components of micelles
  2. Make fatty acids/monoglycerides (more) soluble (in water)
    OR
    Bring/release/carry fatty acids/monoglycerides to cell/lining (of the iluem)
    OR
    Maintain high(er) concentration of fatty acids/monoglycerides to cell/lining (of the ileum);
    Accept lipid/fat for fatty acid/ monoglyceride
  3. Fatty acids/monoglycerides absorbed by diffusion;
    Reject if absorbed by facilitated diffusion
    Ignore if micelles themselves are being absorbed
  4. Triglycerides (re)formed (in cells);
    Accept chylomicrons form
  5. Vesicles move to cell membrane;
    Accept exocytosis for ‘vesicles move’
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24
Q

The action of the carrier protein X in Figure 1 is linked to a membrane-bound ATP hydrolase enzyme.
Explain the function of this ATP hydrolase. (2 marks)

A
  1. (ATP to ADP + Pi ) Releases energy;
    Reject ‘produces/makes/creates energy’.
  2. (energy) allows ions to be moved against a concentration gradient
    OR
    (energy) allows active transport of ions;
    For ‘ions’ accept Na+ or K+.
    Do not accept if this movement is of glucose not ions.
25
Q

The movement of Na+ out of the cell allows the absorption of glucose into
the cell lining the ileum.
Explain how. (2 marks)

A
  1. (Maintains/generates) a concentration/diffusion gradient for Na+ (from ileum into cell);
    Accept ‘(Maintains/generates) a lower concentration of Na+ inside the cell compared with outside the cell’.
  2. Na+ moving (in) by facilitated diffusion, brings glucose with it
    OR
    Na+ moving (in) by co-transport, brings glucose with it;
    Accept ‘co-transporter’ for ‘co-transport’.
26
Q

Describe the role of micelles in the absorption of fats into the cells lining the ileum. (3 marksj

A
  1. Micelles include bile salts and fatty acids;
    Ignore other correct components of micelles.
  2. Make the fatty acids (more) soluble in water;
    For ‘fatty acids’ accept fats / lipids.
  3. Bring/release/carry fatty acids to cell/lining (of the ileum);
    For ‘fatty acids’ accept fats/lipids.
  4. Maintain high(er) concentration of fatty acids to cell/lining (of the ileum);
  5. Fatty acids (absorbed) by diffusion;
    Reject if absorbed by facilitated diffusion
    Ignore if micelles themselves are being absorbed. Ignore references to monoglycerides.
27
Q

Describe the role of enzymes in the digestion of proteins in a mammal. (4 marks)

A
  1. (Reference to) hydrolysis of peptide bonds;
  2. Endopeptidase act in the middle of protein/polypeptide
    OR
    Endopeptidase produces short(er) polypeptides/ increase number of ends;
  3. Exopeptidases act at end of protein/polypeptide
    OR
    Exopeptidase produces dipeptides/amino acids;
  4. Dipeptidase acts on dipeptide/between two amino acids OR
    Dipeptidase produces (single) amino acids;

Accept chain/chain of amino acids/peptide for polypeptide Accept digest/breakdown/ break for ‘act’
Mark points 2, 3 and 4 reject answers where substrate or product is incorrect eg ‘Endopeptidase produces dipeptides’
Ignore references to source and location of enzymes

28
Q

Tick (✔) the box by the name of the process by which fatty acids and glycerol enter the intestinal epithelial cell. (1)

A

Diffusion

29
Q

Explain the advantages of lipid droplet and micelle formation. (3 marks)

A
  1. Droplets increase surface areas (for lipase / enzyme action);
  2. (So) faster hydrolysis / digestion (of triglycerides / lipids);
  3. Micelles carry fatty acids and glycerol / monoglycerides to / through membrane / to (intestinal epithelial) cell;
30
Q

Name structure Q in the diagram above and suggest how it is involved in the absorption of lipids. (4 marks)
Structure Q is Golgi apparatus

A
  1. Modifies / processes triglycerides;
  2. Combines triglycerides with proteins;
  3. Packaged for release / exocytosis
    OR
    Forms vesicles;
    Ignore ‘process
31
Q

Describe how the structure of a protein depends on the amino acids it contains

A
  1. Structure is determined by (relative) position of amino acid/R group/interactions;
    Accept for ‘interactions’, hydrogen bonds / disulfide bridges / ionic bonds / hydrophobichydrophilic interactions
  2. Primary structure is sequence/order of amino acids;
  3. Secondary structure formed by hydrogen bonding (between amino acids);
    Accept alpha helix/β-pleated sheet for ‘secondary structure’
  4. Tertiary structure formed by interactions (between R groups);
    Accept for ‘interactions’, hydrogen bonds / disulfide bridges / ionic bonds / hydrophobichydrophilic interactions
  5. Creates active site in enzymes
    OR
    Creates complementary/specific shapes in antibodies/carrier proteins/receptor (molecules);
  6. Quaternary structure contains >1 polypeptide chain
    OR
    Quaternary structure formed by interactions/bonds between polypeptides;
    Accept for ‘intereactions’, hydrogen bonds/ disulfide bridges/ionic bonds/hydrophobichydrophilic interactions
    Accept prosthetic (group)
32
Q

Role of roots

A

-absorbs water by osmosis and minerals by active transport
-plants need water for photosynthesis, cytoplasm hydration, turgidity of cells
-plants need magnesium, nitrate, phosphate (magnesium to make chlorophyll, nitrate to make amino acids, phosphate to make phospholipids/ATP/DNA)

33
Q

Function and structure of xylem

A

-transports water and mineral from roots up the plant to the leaves
-long continuous hollow tube (no resistance to water flow)
-narrow lumen
-wall made out of lignin
-lignin: strong, waterproof, adhesive
-wall contains pits/pores (water and minerals can leave)

34
Q

How does water move up the xylem?

A

-water is lost from leaves by transpiration
-water moves from top of xylem into leaf by osmosis (transpiration stream)
-this applies TENSION to the column of water in the xylem
-the column of water moves up as one as the water particles stick together
-this is is the cohesion-tension theory
-it is supported by capillary action, adhesion and root pressure
-(capillary action = water automatically moves up narrow lumen of xylem)
-(adhesion = water particles stick to lignin in wall of xylem)
-(root pressure = water absorbed at the roots pushes the column of water up slightly by hydrostatic pressure)

The water vapour lost by transpiration lowers the water potential in the air spaces surrounding the mesophyll cells
The water within the mesophyll cell walls evaporates into these air spaces resulting in a transpiration pull
This transpiration pull results in water moving through the mesophyll cell walls or out of the mesophyll cytoplasm
Movement of water through the cell walls of a plant is said to occur via the apoplast, or apoplastic, pathway
Movement of water from the cytoplasm of a cell occurs via the symplast, or symplastic, pathway
The pull from the water moving through the mesophyll cells results in water leaving the xylem vessels through pits (non-lignified areas), which then causes water to move up the xylem vessels to replace this lost water (due to the cohesive and adhesive properties of the water). This movement is called the transpiration stream

35
Q

Why does tree diameter decrease during the day?

A

-more light and higher temperature
-increase rate of transpiration
-increase transpirational pull
-water pulled up xylem by cohesion-tension
-because the water particles stick to the wall of the xylem (adhesion)
-the walls of the xylem are pulled inwards

36
Q

Adaptations of palisade cells for photosynthesis?

A

-located near top of leaf, closer to light
-large size, large surface area for light
-thin cell wall, short diffusion distance for carbon dioxide
-contains many chloroplasts, site of photosynthesis
-large vacuole, pushes chloroplast to the edge of the cell closer to light

37
Q

How transpiration occurs and factors that increase it

A

-loss of water vapour from the leaf via the stomata

-moist lining of spongy mesophyll cells evaporate forming water vapour
-water vapour builds up in air spaces
-if water vapour concentration is high enough & stomata is open, water vapour diffuses out

Factors:
-light = more light, more stomata open, increase surface area for transpiration
-temperature = more temperature, more evaporation (increase water vapour concentration) & more kinetic energy
-wind = more wind, maintains concentration gradient
-humidity = less humidity, less water vapour in the surrounding air, increase in water vapour concentration gradient

38
Q

What is a potometer?
How do you set it up?

A

-apparatus that indirectly measures rate of transpiration by measuring rate of water uptake as a result of water loss from plant

-as transpiration occurs from leaves, plant pulls up more water from potometer by cohesion-tension
-causes the bubble to move towards the plant
-the more water lost by transpiration, the more water taken up, the further the bubble moves

-rate of transpiration = volume of transpiration divided by time
-volume of transpiration = distance bubble moved x cross-sectional area of tube (πr2)

Method:
-choose healthy leaf and shoot
-cut shoot underwater and connect to potometer underwater (prevents air bubbles entering/blocking xylem)
-ensure potometer is air tight and water tight

39
Q

Function and structure of phloem

A

-transports dissolved sugars up and down a plant
-made of sieve tubes with companion cells

40
Q

How does phloem transport organic material like sucrose?

A

-by Mass Flow (mass flow of water carries the sucrose)
-Sucrose loaded into Phloem at Source
-Hydrogen Ions (H+) actively transported from companion cells into source
-therefore, H+ diffuses back into companion cells from source
-as they do, they pull in sucrose with them by co-transport
-sucrose then diffuses into sieve tube
-this lowers the water potential of sieve tube so water follows by osmosis
-this water will carry the sucrose by hydrostatic pressure (mass flow)

-sucrose produced by the source diffuses into companion cells by facilitated diffusion
-sucrose is actively transported from companion cells into sieve tube elements using ATP.
-LOADING of sucrose into phloem decreases water potential of the sieve tubes
-causes water from xylem to diffuse into phloem by osmosis
-increases hydrostatic pressure of phloem
-near the sink, sucrose is either used up or converted to starch for storage
-decreases the water potential of the sink.
-hence water diffuses from sieve tubes into sink by osmosis
-sucrose moves from phloem to sink by diffusion, known as UNLOADING
-decreases hydrostatic pressure in the phloem

41
Q

Describe how oxygen in the air reaches capillaries surrounding alveoli in the lungs. Details of breathing are not required (4)

A

Air travels through the trachea, bronchi, and bronchioles to reach alveoli, down a pressure gradient. Then, oxygen travels down a diffusion gradient through the alveolar epithelium

42
Q

Structure and function of haemoglobin

A

-quaternary globular protein made of 4 polypeptide chains (2α, 2β)
-each chain carries a haem group
-each haem group carries Fe2+
-each Fe2+ carries an O2
-therefore, each haemoglobin carries 4 O2 molecules

Role is to load O2 in the lungs and deliver it to the respiring tissues

43
Q

The role of haemoglobin and red blood cells in the transport of oxygen

A

-haemoglobin has High Affinity in the lungs
-due to high partial pressure of oxygen and low partial pressure of carbon dioxide
-so haemoglobin loads/associates oxygen in the lungs and becomes saturated (full)
-the haemoglobin is transported in the blood in the red blood cell
-at the respiring tissues, haemoglobin has Low Affinity
-due to low partial pressure of oxygen and high partial pressure of carbon dioxide
-so oxygen is unloaded/dissociated and haemoglobin becomes unsaturated

44
Q

The loading, transport and unloading of oxygen in relation to the oxyhaemoglobin dissociation curve. The cooperative nature of oxygen binding to show that the change in shape of haemoglobin caused by binding of the first oxygens makes the binding of further oxygens easier.

A

Relationship between O2 Partial Pressure & Affinity/Saturation of Haemoglobin?
-positive correlation
-as O2 partial pressure increases, affinity/saturation of haemoglobin increases
-the correlation produces a s-shaped, sigmoid curve called Oxygen Dissociation Curve
-middle portion of ODC has a steep gradient so when respiring tissues change from

Relationship between CO2 Partial Pressure & Affinity/Saturation of Haemoglobin?
-negative correlation
-as CO2 partial pressure increases, affinity/saturation of haemoglobin decreases
-occurs at site of respiring tissues
-carbon dioxide lowers pH of blood
-makes the haemoglobin change shape
-so oxygen is released
-lowers affinity
-which shifts the ODC to the right, called the bohr shift
-benefit = more oxygen delivered to respiring cells

45
Q

How does a fetus receive oxygen? Benefit of this? Why do adults not have this?

A

-from mother’s blood
-oxygen dissociates from mother’s haemoglobin and associates with fetal haemoglobin in the placenta
-fetal haemoglobin has a higher affinity compared to mother’s haemoglobin

Benefit?
-fetal haemoglobin’s ODC will be to the left
-has high affinity, so the oxygen will dissociate from mother’s haemoglobin and associate with fetal haemoglobin at low partial pressures of oxygen in the placenta
-so it has enough oxygen for its needs
-adults don’t have this, as high affinity will mean less oxygen will be unloaded at the respiring tissues

46
Q

Affinity of organisms in a low oxygen environment?

Affinity of active organisms?

Affinity of small organisms?

A

Low O2 environment
-has a high affinity
-curve to the left
-hence it can readily associate oxygen at the low oxygen partial pressures

Active organisms?
-has a low affinity
-curve to the right
-hence more oxygen can be unloaded to meet the cell’s demand for more respiration

Small organisms?
-have a large surface area to volume ratio
-lose more heat
-needs to respire to generate heat
-hence has a low affinity
-curve to the right
-so unloads enough oxygen for the cells’ demand of more respiration

47
Q

How is tissue fluid formed and returned to circulatory system?

A

-at the start of the capillary (arterial end) there is a build up of hydrostatic pressure
-this pushes fluid out of the capillary via the pores
-the fluid carries the nutrients with it
-the fluid surrounds the cells, this is called tissue fluid
-at the end of the capillary (venous end) the fluid moves back in by osmosis
-the capillary has low water potential due to the presence of proteins (too large to move out of capillaries)
-any excess tissue fluid is picked up by the lymph system and deposited in the vena cava

48
Q

adaptations of insects to minimise water loss

A

-small sa:v ratio where water can evaporate from
-exoskeleton made of fibrous, waterproof material and a lipid layer
-spiracles where gases can enter from and water can be evaporated from, can close/open as necessary

49
Q

adaptations of insects for efficient diffusion

A

-large number of fine tracheoles
-walls of tracheoles are thin and short distance between spiracles and tracheoles, hence shorter diffusion pathway
-steep diffusion gradient set up by use of oxygen and production of carbon dioxide

50
Q

Why does hydrostatic pressure falls from the arteriole end of the capillary to the venule end of the capillary

A

Loss of fluid

51
Q

Acronym for valves

A

LAB RAT
Left Atrium = Bicuspid
Right Atrium = Tricuspid

52
Q

Outline the cardiac cycle stages

Formula for cardiac output:

A

Atrial systole:
-atria contracts to ensure remaining blood is emptied from atria and enters the ventricles
-causes ventricular pressure to slightly increase
-ventricular walls remain relaxed (ventricular diastole)
-AV valves remain open as pressure in atria is greater than pressure in ventricles
-semi-lunar valves remain closed to prevent blood flowing out of ventricles too early

Ventricular systole / atrial diastole:
-ventricles contract
-ventricular pressure increases and exceeds pressure in atria
-causes the AV valves to shut, to prevent the backflow of blood back to the atria
-pressure in ventricles rises further
-once it exceeds pressure in aorta and pulmonary artery, blood is forced from ventricles into these vessels through semilunar valves

Diastole:
-both atria and ventricles are relaxed
-blood enters atria at low pressure through the pulmonary vein and vena cava
-volume of atria increases
-causes the blood pressure to increase
-causes AV valves to become open so blood enters ventricles
-muscular walls of atria and ventricles relax
-relaxation of ventricle walls causes them to recoil and reduces pressure in ventricle
-lower pressure in ventricle compared to that of aorta and pulmonary artery
-hence semi-lunar valves in the aorta and pulmonary artery close

Cardiac output = heart rate x stroke volume
Stroke volume = vol of blood that leaves per heartbeat

53
Q

Outline the general pattern of blood circulation in a mammal

A
54
Q

Names are required only of the coronary arteries and of the blood vessels entering and leaving the heart, lungs and kidneys

A
55
Q

Outline how the structure of an artery is related to its function

A

Function: carries blood away from heart

-narrow lumen maintains pressure

-smooth lining made of squamous epithelial cells reduces friction

-thick wall = withstand high blood pressure

-elastic tissue in wall
-ventricles contract = elastic tissue stretches to withstand pressure
-ventricles relax = elastic tissue recoils to maintain pressure and smooth out flow

-smooth muscle in wall (particularly in arterioles),
-smooth muscle contracts – lumen narrows and arteriole constricts
-smooth muscle relaxes – lumen widens and arteriole dilates

-collagen in wall prevents artery from tearing

56
Q

Outline how the structure of a capillary is related to its function

Outline the importance of capillary beds as exchange surfaces

A

-single layer of epithelial cells = thin wall = shorter diffusion distance
-highly branched = increased total surface area
-very small lumen to maintain pressure

57
Q

Outline how the structure of a vein is related to its function

A

return blood back to the heart under low pressure

-wide lumen = ease of blood flow
-smooth lining made of squamous epithelial cells to reduce friction
-thin wall = vein can be squashed by skeletal muscle pushing blood back to the heart
-valves in lumen = prevents backflow of blood

58
Q

Topic 3/4 test main mistakes

A

-counter-current flow maintains diffusion gradient along the length of the gill filament
-loss of fluid / loss of water
-fluid is not pushed out; water is pushed out of capillary
-alveoli are one cell thick, do not say thin wall/membrane