3.2 Morphology

Question 1

• Description: Broad-based budding
  yeasts (10-15 microns in size)
• Diagnosis: Broad-Based budding
  yeasts

a. Cryptococcus spp.
b. Blastomyces dermititidis
c. Histoplasma capsulatum
d. Coccidioides spp.
e. Pneumocystis pneumonia
f. Penicillim marneffei
g. Paracoccidioides
h. Sporothrix schenckii

Answer 1

b. Blastomyces dermititidis

Question 2

• Description: Narrow-based
  budding yeasts (4-10 microns in
  size) with a thick mucicarmine
  positive capsule and positive with
  melanin stains (Fontana Masson).
• Diagnosis: Narrow-based budding
  yeasts with mucicarmine positive
  capsule.

a. Cryptococcus spp.
b. Blastomyces dermititidis
c. Histoplasma capsulatum
d. Coccidioides spp.
e. Pneumocystis pneumonia
f. Penicillim marneffei
g. Paracoccidioides
h. Sporothrix schenckii

Answer 2

a. Cryptococcus spp.

Question 3

Description: Small yeasts (2-4
microns in size) with narrow based
budding grouped in clusters inside
macrophages

• Diagnosis: Small yeasts with
  narrow based budding

a. Cryptococcus spp.
b. Blastomyces dermititidis
c. Histoplasma capsulatum
d. Coccidioides spp.
e. Pneumocystis pneumonia
f. Penicillim marneffei
g. Paracoccidioides
h. Sporothrix schenckii

Answer 3

c. Histoplasma capsulatum

Question 4

• Description: Spherules with
  multiple endospores (10 to 100
  microns in size).
• Diagnosis: Spherules with multiple
  endospores.

a. Cryptococcus spp.
b. Blastomyces dermititidis
c. Histoplasma capsulatum
d. Coccidioides spp.
e. Pneumocystis pneumonia
f. Penicillim marneffei
g. Paracoccidioides
h. Sporothrix schenckii

Answer 4

d. Coccidioides spp.

Question 5

• Description: Thin-walled spheres
  (2 to 5 microns in size) with
  intracystic foci. In lung they appear
  as foamy intraalveolar eosinophilic
  exudates. The diagnosis is
  problematic when they are
  extrapulmonary and can be
  confused with Histoplasma

a. Cryptococcus spp.
b. Blastomyces dermititidis
c. Histoplasma capsulatum
d. Coccidioides spp.
e. Pneumocystis pneumonia
f. Penicillim marneffei
g. Paracoccidioides
h. Sporothrix schenckii

Answer 5

e. Pneumocystis pneumonia

Question 6

Small oval-shaped
yeasts (2 to 5 microns in diameters)
with tranverse septum (due to
division by fission).

a. Cryptococcus spp.
b. Blastomyces dermititidis
c. Histoplasma capsulatum
d. Coccidioides spp.
e. Pneumocystis pneumonia
f. Penicillium marneffei
g. Paracoccidioides
h. Sporothrix schenckii

Answer 6

f. Penicillium marneffei

Question 7

Variable size yeasts
(4 to 60 microns in size) with
multiple buds surrounding the
parent cell. Described as “pilot
wheel”. Some cells may only have
1 or 2 buds and should not be
confused with other yeasts such as
Sporothrix or Cryptococcus

a. Cryptococcus spp.
b. Blastomyces dermititidis
c. Histoplasma capsulatum
d. Coccidioides spp.
e. Pneumocystis pneumonia
f. Penicillium marneffei
g. Paracoccidioides
h. Sporothrix schenckii

Answer 7

g. Paracoccidioides

Question 8

• Description:Asteroid bodies (starlike eosinophilic material surrounding yeasts or yeast-like
  structures) are found in up to 92%
  of sporotrichosis cases. Less
  frequently found are round, oval or
  cigar shaped yeasts (2 to 6 microns
  in diameter or larger) (inset) with
  narrow based or tube-like budding
  (budding not present in the inset).
• Diagnosis: Asteroid body and
  small oval yeast (with narrow based
  budding -if present)

a. Cryptococcus spp.
b. Blastomyces dermititidis
c. Histoplasma capsulatum
d. Coccidioides spp.
e. Pneumocystis pneumonia
f. Penicillium marneffei
g. Paracoccidioides
h. Sporothrix schenckii

Answer 8

h. Sporothrix schenckii

Question 9

• Description: Small yeasts (3- 5
  microns in size)
• Diagnosis: Yeasts with
  pseudohyphae.

a. Mucorales genera
b. Dematiaceous fungi
c. Aspergillus
d. Candida spp.

Answer 9

d. Candida spp.

Question 10

• Description: Non-pigmented
  (hyaline), septated hyphae with acute
  angle branching
• Diagnosis: Non-pigmented (hyaline),
  septated hyphae

a. Mucorales genera
b. Dematiaceous fungi
c. Aspergillus
d. Candida spp.

Answer 10

c. Aspergillus

Question 11

• Description: Non-pigmented
  (hyaline), pauciseptate ribbon-like
  hyphae with right angle branching.
• Diagnosis: Non-pigmented (hyaline),
  pauciseptate hyphae

a. Mucorales genera
b. Dematiaceous fungi
c. Aspergillus
d. Candida spp.

Answer 11

a. Mucorales genera

Question 12

• Description: Pigmented irregular
  hyphae and yeast-like structures both
  with septations.
• Diagnosis: Pigmented yeasts and
  hyphae with septations.

a. Mucorales genera
b. Dematiaceous fungi
c. Aspergillus
d. Candida spp.

Answer 12

b. Dematiaceous fungi