1.1 Classification and Lab diagnosis of Fungi Flashcards
1
Q
What fungi is unicellular?
A
Yeasts
2
Q
What fungi is multicellular?
A
Mold
3
Q
Cell wall of fungi is made of?
A
Chitin
Glucans
Mannans
4
Q
What type of organisms is fungi?
a. chemoheterotroph
b. Heterotroph
c. Chemotroph
d. Thermotroph
A
a.
(Feeds on other organisms to gain energy)
5
Q
Fungi is __________ because to secretes exoenzymes into the environment than absorb the digested nutrients
A
Absorptive
6
Q
Fungi can metabolize complex carbohydrates
T or F
A
T
7
Q
Fungi requires what type of oxygen requirement?
A
Aerobic
Facultative anaerobic
8
Q
Fungi are motile
T or F
A
F
9
Q
Fungi only do asexual reproductive cycles
T or F
A
F
Can perform sexual
10
Q
Fungi are resistant to osmotic pressure
A
T
11
Q
What pH for fungi to grow?
A
pH 5
12
Q
A unicellular fungi with blastogenesis characterstics
A
Yeasts
13
Q
What is the incubation temp for Yeast?
A
37
14
Q
A multicellular fungi that can produce spores
A
MOlds
15
Q
What is the temp require for molds?
A
22 (RT)
16
Q
Hyphae (Mycelium)
Thallus (Aerial hyphae)
T or F
A
T
17
Q
Example of Yeast fungi?
A
Candida
Cryptococcus
18
Q
Example of Mold fungi?
A
Dermatophytes
Zygomytes
Aspergillus
Penicillium
19
Q
Example of dimorphic (Can be yeast or Mold)
A
Sporothrix
Histoplasma
Blastomyces
Paracoccidiodes
Coccidioides
20
Q
What do you call a hyphae that is submerge in the agar?
A
Vegetative hyphae
21
Q
What do you call a hyphae that is Floating or above on the agar?
A
Aerial hyphae
22
Q
What is the components/type of hyphae of mold for zygomycetes (Mucor, Rhizopus)
A
Coenocytic/Aseptate
23
Q
What is the components of mold for Majority of fungi such as dermatophytes?
A
Septate
24
Q
Fungi capable of sexual reproduction (Fruiting body)
A
Perfect Fungi (Telemorph)
25
Are formed from the fusion of nuclei from two opposite mating strains of the same species
Sexual spores
26
Example of sexual spores?
Ascospores
Basidiospores
Zygospores
Oospores
27
Fungi that lacks sexual reproduction?
More on identification
Fungi imperfecti (Anamorph)
28
Example of anamorph
Penicillium
Aspergillus
29
Most common bread mold?
Conjugation spore (sexual and asexual)
Zygomycota
30
What is the sexual spore for zygomycota?
Zygospore
31
What is the asexual spore for zygmycota?
Sporangiospore
32
What fungi have a hyphae with sparsely septate or aseptate and is known as conjugation fungi
Zygomycota
33
Part of zygomycota that is known as black bread mold?
Rhyzopus nigricans
34
Other examples of zygomycota:
Mucor
Absidia
Cunninghumella
35
Fungi with septate hyphae that is parasite for plants?
Ascomycota
36
Name of sac for ascomycota?
Ascus
37
Sexual spore for ascomycota
Ascospore
38
Asexual spore for ascomycota?
Conidiospore
39
A club fungi with septate hyphae that produces mushrooms
Basidiomycota
40
What is the sexual spore for Basidiomycota
Basidiospore
41
What is the asexual spore for Basidiomycota?
conidiospore
42
Example of basidiomycota?
Cryptococcus neoformans
43
Conidia borne on condiophores are asexual spores?
T or F
T
44
Ascomycota are fungi imperfecti
T or F
T
45
In medical mycology, the classification of each fungi are according to site of infection which are
Superficial
Cutaneous
Subcutaneous
Systemic/Deep
Opportunistic
T or F
T
46
gene of fungi that has the ability to adhere to host cell by glycoproteins
HYP1 gene
47
What are the enzyme produce during fungal pathogenicity
Elastase
Collagenase
48
What stains is used to detect fungi in CSF?
India ink
49
What reagent/stain is used to detectect dermatophytes?
10-20% KOH
50
How many hours to incubate dermatophytes?
72 hrs
51
This fungi has characteristics of:
Skin - Advancing border
Hair - Basal and distorted/fractured hair
Nail - under material
Dermatophytes
52
What container used to collect fungi?
Heparin tube
Blood culture bottle
53
To collect fungi in CSF:
2mL sample
spin 2000 rpm for 10mins
If less than 2mL sample, direct culture or smear
T or F
T
54
Abcess, pus, Exudate with granules are done in direct exam
T or F
T
55
Exduate w/o granules: no pre treatment
T or F
T
56
Mycotic keratitis happens in what part of the body?
Eye
57
Mycotic keratitis is collected through direct inoculation in agar?
T or F
F
Indirect
58
How to collect fungi in the ear?
Dacron
59
what is the temp when collecting samples in the upper respiratory system in less than 2 hrs?
RT
60
what is the temp when collecting samples in the upper respiratory system in More than 2 hrs?
RT
61
what is the temp when collecting samples in the Lower resp/Sputum/Lavage in More than 2 hrs?
4C
62
what is the temp when collecting samples in the Lower resp/Sputum/Lavage in Less than 2 hrs?
RT
63
Sputum is subjected to NALC for preservation?
T or F
T
64
Sputum is inoculate for 3-4 days
T or F
T
65
Detecting fungi in the sputum is collected in?
1st morning specimen
66
If sample is collected in 24 hrs during collection, it can be accepted
T or F
F
67
Less than 2hrs for urine, what is the temp
RT
68
More than 2hrs for urine, what is the temp?
4C
69
How to collect sample in the vagina? for candidiasis and moniliasis
Paps
70
In identifying fungi, larger tissue fragment is taken to the?
Bacteriology section
71
Tissue:
W/o NSS = <15 mins RT
w/NSS: up to 24hrs RT
T or F
T
72
How to prevent drying for tissue
NSS cover
73
Clearing reagent for wet mount?
10-20% KOH
74
Reagent that binds with chitin and cellulose; fluoresces with uv light
Calcofluor white
75
What stain to detect encapsulated yeasts?
India ink/ Nigrosin
76
Yeasts Fungi with red pigment characteristics
Rhodoturula
77
Brand name that is selective for yeast
Mycosel
78
Part of mycosel that is used for antibacterial
a. Chloramphenicol
b. Cycloheximide
Chloramphenicol
79
Part of mycosel that is used to inhibit C. neoformans/gatti
a. Chloramphenicol
b. Cycloheximide
b
80
Yeast that is pink in urease test?
Cryptococcus
81
Without the use of antibacterial, which primary isolation enhances reproductive structure colony color?
a. SDA
b PDA
c. BHI
a and b
82
Without the use of antibacterial, which primary isolation enhances growth of dimorphic fungi
a. SDA
b PDA
c. BHI
c
83
Dermatophyte test medium with ____ pH is used to identify dermatophytes
High
84
Stains for mold:
LPCB
PVA
H & E
PAS
GMS
Meyer's Mucicarmine
Fluorescent Ab staining
Which of the following use to detect C. neoformancs with rose to red color
Meyer's mucicarmine
85
Stains for mold:
LPCB
PVA
H & E
PAS
GMS
Meyer's Mucicarmine
Fluorescent Ab staining
Which of the following use to see structures
H & E
86
Stains for mold:
LPCB
PVA
H & E
PAS
GMS
Meyer's Mucicarmine
Fluorescent Ab staining
Which of the following use to produce red/purple color
PAS
87
In culture media: which of the following days used for general incubation?
a. 7 days
b. 14 days
c. 21 days
d. 28 days
b
88
In culture media: which of the following days used to detect presence of yeast in the mouth, throat or vagina
a. 7 days
b. 14 days
c. 21 days
d. 28 days
a
89
In culture media: which of the following days used tissues and sterile body fluids other than blood?
a. 7 days
b. 14 days
c. 21 days
d. 28 days
c
90
In culture media: which of the following days used for respiratory, BM, blood specimens, and specimens in which dimorphic fungus are suspected
a. 7 days
b. 14 days
c. 21 days
d. 28 days
d
91
Most molds Grow best at?
a. 25 - 27C
b. 30 - 32C
c. 35 - 37C
d. 25 - 30C
d
92
Most pathogenic fungi Grow best at?
a. 25 - 27C
b. 30 - 32C
c. 35 - 37C
d. 25 - 30C
b
93
Most yests Grow best at?
a. 25 - 27C
b. 30 - 32C
c. 35 - 37C
d. 25 - 30C
c
94
Sporothrix schenckii?
a. 25 - 27C
b. 30 - 32C
c. 35 - 37C
d. 25 - 30C
a
95
In SDA what pH tht prevents bacterial growth?
5.6
96
BHI is used for pathogenic fungi?
T or F
F
Fastidious fungi
97
IMA or Inhibitry mold agar is used for pathogenic fungi?
T or F
T
98
PDA or potato dextrose agar is used for mold sporulation and pigmet production in dermatophytes
T or F
T
99
General used - SDA, BHIA, SABHI
Selective - With antibiotics (IMA, DTM, Chrom Agar)
Differential - Enhance pigment deveopment
T or F
T
100
Birdseed agar - C. neoformans (Black brown colonies
T or F
T
101
Cottonseed agar - B dermatitidis from mold to yeast
T or F
T
102
Cornmeal Tween 80 - Differentiates Candida
T or F
T
103
What is used for preservation of cultures for fungi?
Formalin
104
Temp for Deep freeze?
-20 - -70
