Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Mycoviro (Finals) > 1.1 Classification and Lab diagnosis of Fungi > Flashcards

1.1 Classification and Lab diagnosis of Fungi Flashcards

1
Q

What fungi is unicellular?

A

Yeasts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What fungi is multicellular?

A

Mold

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Cell wall of fungi is made of?

A

Chitin

Glucans

Mannans

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What type of organisms is fungi?

a. chemoheterotroph
b. Heterotroph
c. Chemotroph
d. Thermotroph

A

a.

(Feeds on other organisms to gain energy)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Fungi is __________ because to secretes exoenzymes into the environment than absorb the digested nutrients

A

Absorptive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Fungi can metabolize complex carbohydrates

T or F

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Fungi requires what type of oxygen requirement?

A

Aerobic

Facultative anaerobic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Fungi are motile

T or F

A

F

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Fungi only do asexual reproductive cycles

T or F

A

F

Can perform sexual

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Fungi are resistant to osmotic pressure

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What pH for fungi to grow?

A

pH 5

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

A unicellular fungi with blastogenesis characterstics

A

Yeasts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the incubation temp for Yeast?

A

37

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

A multicellular fungi that can produce spores

A

MOlds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the temp require for molds?

A

22 (RT)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Hyphae (Mycelium)
Thallus (Aerial hyphae)

T or F

A

T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Example of Yeast fungi?

A

Candida

Cryptococcus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Example of Mold fungi?

A

Dermatophytes

Zygomytes

Aspergillus

Penicillium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Example of dimorphic (Can be yeast or Mold)

A

Sporothrix

Histoplasma

Blastomyces

Paracoccidiodes

Coccidioides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What do you call a hyphae that is submerge in the agar?

A

Vegetative hyphae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What do you call a hyphae that is Floating or above on the agar?

A

Aerial hyphae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What is the components/type of hyphae of mold for zygomycetes (Mucor, Rhizopus)

A

Coenocytic/Aseptate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What is the components of mold for Majority of fungi such as dermatophytes?

A

Septate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Fungi capable of sexual reproduction (Fruiting body)

A

Perfect Fungi (Telemorph)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Are formed from the fusion of nuclei from two opposite mating strains of the same species

A

Sexual spores

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Example of sexual spores?

A

Ascospores

Basidiospores

Zygospores

Oospores

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Fungi that lacks sexual reproduction?

More on identification

A

Fungi imperfecti (Anamorph)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Example of anamorph

A

Penicillium

Aspergillus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Most common bread mold?

Conjugation spore (sexual and asexual)

A

Zygomycota

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

What is the sexual spore for zygomycota?

A

Zygospore

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

What is the asexual spore for zygmycota?

A

Sporangiospore

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

What fungi have a hyphae with sparsely septate or aseptate and is known as conjugation fungi

A

Zygomycota

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

Part of zygomycota that is known as black bread mold?

A

Rhyzopus nigricans

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Other examples of zygomycota:

Mucor
Absidia
Cunninghumella

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

Fungi with septate hyphae that is parasite for plants?

A

Ascomycota

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

Name of sac for ascomycota?

A

Ascus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

Sexual spore for ascomycota

A

Ascospore

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

Asexual spore for ascomycota?

A

Conidiospore

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

A club fungi with septate hyphae that produces mushrooms

A

Basidiomycota

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

What is the sexual spore for Basidiomycota

A

Basidiospore

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

What is the asexual spore for Basidiomycota?

A

conidiospore

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

Example of basidiomycota?

A

Cryptococcus neoformans

43
Q

Conidia borne on condiophores are asexual spores?

T or F

A

T

44
Q

Ascomycota are fungi imperfecti

T or F

A

T

45
Q

In medical mycology, the classification of each fungi are according to site of infection which are

Superficial
Cutaneous
Subcutaneous
Systemic/Deep
Opportunistic

T or F

A

T

46
Q

gene of fungi that has the ability to adhere to host cell by glycoproteins

A

HYP1 gene

47
Q

What are the enzyme produce during fungal pathogenicity

A

Elastase

Collagenase

48
Q

What stains is used to detect fungi in CSF?

A

India ink

49
Q

What reagent/stain is used to detectect dermatophytes?

A

10-20% KOH

50
Q

How many hours to incubate dermatophytes?

A

72 hrs

51
Q

This fungi has characteristics of:
Skin - Advancing border
Hair - Basal and distorted/fractured hair
Nail - under material

A

Dermatophytes

52
Q

What container used to collect fungi?

A

Heparin tube

Blood culture bottle

53
Q

To collect fungi in CSF:
2mL sample
spin 2000 rpm for 10mins

If less than 2mL sample, direct culture or smear

T or F

A

T

54
Q

Abcess, pus, Exudate with granules are done in direct exam

T or F

A

T

55
Q

Exduate w/o granules: no pre treatment

T or F

A

T

56
Q

Mycotic keratitis happens in what part of the body?

A

Eye

57
Q

Mycotic keratitis is collected through direct inoculation in agar?

T or F

A

F

Indirect

58
Q

How to collect fungi in the ear?

A

Dacron

59
Q

what is the temp when collecting samples in the upper respiratory system in less than 2 hrs?

A

RT

60
Q

what is the temp when collecting samples in the upper respiratory system in More than 2 hrs?

A

RT

61
Q

what is the temp when collecting samples in the Lower resp/Sputum/Lavage in More than 2 hrs?

A

4C

62
Q

what is the temp when collecting samples in the Lower resp/Sputum/Lavage in Less than 2 hrs?

A

RT

63
Q

Sputum is subjected to NALC for preservation?

T or F

A

T

64
Q

Sputum is inoculate for 3-4 days

T or F

A

T

65
Q

Detecting fungi in the sputum is collected in?

A

1st morning specimen

66
Q

If sample is collected in 24 hrs during collection, it can be accepted

T or F

A

F

67
Q

Less than 2hrs for urine, what is the temp

A

RT

68
Q

More than 2hrs for urine, what is the temp?

A

4C

69
Q

How to collect sample in the vagina? for candidiasis and moniliasis

A

Paps

70
Q

In identifying fungi, larger tissue fragment is taken to the?

A

Bacteriology section

71
Q

Tissue:

W/o NSS = <15 mins RT
w/NSS: up to 24hrs RT

T or F

A

T

72
Q

How to prevent drying for tissue

A

NSS cover

73
Q

Clearing reagent for wet mount?

A

10-20% KOH

74
Q

Reagent that binds with chitin and cellulose; fluoresces with uv light

A

Calcofluor white

75
Q

What stain to detect encapsulated yeasts?

A

India ink/ Nigrosin

76
Q

Yeasts Fungi with red pigment characteristics

A

Rhodoturula

77
Q

Brand name that is selective for yeast

A

Mycosel

78
Q

Part of mycosel that is used for antibacterial

a. Chloramphenicol
b. Cycloheximide

A

Chloramphenicol

79
Q

Part of mycosel that is used to inhibit C. neoformans/gatti

a. Chloramphenicol
b. Cycloheximide

A

b

80
Q

Yeast that is pink in urease test?

A

Cryptococcus

81
Q

Without the use of antibacterial, which primary isolation enhances reproductive structure colony color?

a. SDA
b PDA
c. BHI

A

a and b

82
Q

Without the use of antibacterial, which primary isolation enhances growth of dimorphic fungi

a. SDA
b PDA
c. BHI

A

c

83
Q

Dermatophyte test medium with ____ pH is used to identify dermatophytes

A

High

84
Q

Stains for mold:
LPCB
PVA
H & E
PAS
GMS
Meyer’s Mucicarmine
Fluorescent Ab staining

Which of the following use to detect C. neoformancs with rose to red color

A

Meyer’s mucicarmine

85
Q

Stains for mold:
LPCB
PVA
H & E
PAS
GMS
Meyer’s Mucicarmine
Fluorescent Ab staining

Which of the following use to see structures

A

H & E

86
Q

Stains for mold:
LPCB
PVA
H & E
PAS
GMS
Meyer’s Mucicarmine
Fluorescent Ab staining

Which of the following use to produce red/purple color

A

PAS

87
Q

In culture media: which of the following days used for general incubation?

a. 7 days
b. 14 days
c. 21 days
d. 28 days

A

b

88
Q

In culture media: which of the following days used to detect presence of yeast in the mouth, throat or vagina

a. 7 days
b. 14 days
c. 21 days
d. 28 days

A

a

89
Q

In culture media: which of the following days used tissues and sterile body fluids other than blood?

a. 7 days
b. 14 days
c. 21 days
d. 28 days

A

c

90
Q

In culture media: which of the following days used for respiratory, BM, blood specimens, and specimens in which dimorphic fungus are suspected
a. 7 days
b. 14 days
c. 21 days
d. 28 days

A

d

91
Q

Most molds Grow best at?

a. 25 - 27C
b. 30 - 32C
c. 35 - 37C
d. 25 - 30C

A

d

92
Q

Most pathogenic fungi Grow best at?

a. 25 - 27C
b. 30 - 32C
c. 35 - 37C
d. 25 - 30C

A

b

93
Q

Most yests Grow best at?

a. 25 - 27C
b. 30 - 32C
c. 35 - 37C
d. 25 - 30C

A

c

94
Q

Sporothrix schenckii?

a. 25 - 27C
b. 30 - 32C
c. 35 - 37C
d. 25 - 30C

A

a

95
Q

In SDA what pH tht prevents bacterial growth?

A

5.6

96
Q

BHI is used for pathogenic fungi?

T or F

A

F

Fastidious fungi

97
Q

IMA or Inhibitry mold agar is used for pathogenic fungi?

T or F

A

T

98
Q

PDA or potato dextrose agar is used for mold sporulation and pigmet production in dermatophytes

T or F

A

T

99
Q

General used - SDA, BHIA, SABHI
Selective - With antibiotics (IMA, DTM, Chrom Agar)
Differential - Enhance pigment deveopment

T or F

A

T

100
Q

Birdseed agar - C. neoformans (Black brown colonies

T or F

A

T

101
Q

Cottonseed agar - B dermatitidis from mold to yeast

T or F

A

T

102
Q

Cornmeal Tween 80 - Differentiates Candida

T or F

A

T

103
Q

What is used for preservation of cultures for fungi?

A

Formalin

104
Q

Temp for Deep freeze?

A

-20 - -70

Mycoviro (Finals) (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions