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Mycoviro (Finals) > 3.1 Systemic and Opportunistisc Mycoses > Flashcards

3.1 Systemic and Opportunistisc Mycoses Flashcards

1
Q

Most common opportunistic fungi

a. Candida albicans
b. Pneumocystisis jeoveri
c. Penicillium mameffei
d. M. furfur

A

a.

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2
Q

Type of candida with no chlamydospore

a. Candida albicans
b. Candida dubliniensis
c. Candida tropicalis
d. Candida guilliermondii

A

c

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3
Q

Asexual spore of candida that is within the segment?

a. Intercalary
b. Sessile
c. Terminal
d. Intracalary

A

a

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4
Q

Asexual spore of candida that is hyphal sides?

a. Intercalary
b. Sessile
c. Terminal
d. Intracalary

A

b

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5
Q

Asexual spore of candida that is hyphal tip?

a. Intercalary
b. Sessile
c. Terminal
d. Intracalary

A

c

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6
Q

Candida is a type of yeats cells?

T or F

A

T

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7
Q

Candida has hyphae

T or F

A

F

Pseudohyphae

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8
Q

Candida albicans contains 2 antigenic structure

T or F

A

T

A and B

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9
Q

The antigenic structure of Candida albicans can:

  • Release of cell wall components
  • Local Candidiasis
  • Elaboration of agglutinin

Which of the following is false

A

Local candidiasis

should be “Systemic”

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10
Q

Pathogenesis of C. albicans include:

  • Mucosal candidiasis
  • Chronic mucocutaneous candidiasis
  • Systemic Candidiasis

Which of the following does not belong?

A

None

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11
Q

Critical host defence when it comes to systemic candidiasis is eosinophils?

T or F

A

F

Neutrophils

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12
Q

Media use to culture Candida?

a. CHROMagar
b. BAP
c. TSI
d. SSA

A

CHROMagar

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13
Q

This fungi contains a virulence factors of:

  • Capsule
  • Laccase
  • Urease

a. Crypto. neoformans
b. Crypto. gatti
c. C. albicans
d. C. tropicalis

A

C. neoformans
C. gatti

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14
Q

Fungi that can Absorbed antisera: A, D, or AD

a. Crypto. neoformans
b. Crypto. gatti
c. C. albicans
d. C. tropicalis

A

a

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15
Q

Fungi that can Absorbed antisera: B or C

a. Crypto. neoformans
b. Crypto. gatti
c. C. albicans
d. C. tropicalis

A

b

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16
Q

The antigenic structure of Cryptococcus contains capsular polysaccharide

T or F

A

T

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17
Q

Capsular polysaccharide is insolubilized in CSF, Serum or Urine

T or F

A

F

Soluble

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18
Q

Which Cryptococcus is for immunocompromised patients?

A

C. neoformans

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19
Q

Which Crytptococcus is for immunocompetent patients?

A

C. gatti

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20
Q

Cryptococcus spp. can be transmitted from person to person

T or F

A

F

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21
Q

In Cryptotccocus:
A
CSF protein pressure and protein; cell count is?

High or low

A

High

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22
Q

In Cryptotccocus:
A
CSF glucose is?

High or low

A

Low to normal

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23
Q

Stain use to detect cryptococcus?

a. India ink
b. Wright stain
c. Gram stain
d. Immunofluorescence stain

A

a

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24
Q

Cryptococcus has no hyphae

T or F

A

T

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25
Q

Media used for Cryptococcus? (Confirmatory)

a. CHROMagar
b. BAP
c. CGB agar
d. ABC agar

A

c

Canavanine-glycine-bromthymol blue

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26
Q

In CGB agar,
- Growth
- Medium blue 24-5days

a. C. gatti
b. C. neoformans

A

C. gatti

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27
Q

In CGB agar,
- No growth
- Medum yellow or green

a. C. gatti
b. C. neoformans

A

b.

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28
Q

Culture: 37C (1-2 days)

SDA without cycloheximide: Creamy, white and mucoid

Birdseed agar: Brown to black

Urease: (+)

A

Cryptococcus

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29
Q

Urease positive:

All cryptococcus
Rhodoturula
Trichosporon
Trichophyton mentagrophytes

A
30
Q

Rapidly growing aerial hyphae that with varying long conidiophores with terminal vesicles on which phialides produce basipetal chains of conidia

a. Aspergillus
b. Cryptococcus
c. Rhodococcus
d. Penicillum

A

a

31
Q

Aspergilloma is a fungus ball that colonizes in a healed lung scar or abscess from a previous disease

T or F

A

T

32
Q

Most common Aspergillus?

a. niger
b. flavus
c. fumigatus

A

c

33
Q

What fungi colonizes a recently healed lungs?

A

Aspergillus (Aspergilloma)

34
Q

Mucormycoses:

  • Rhinocerebral mucomycoses
  • Non septate

Which of the following is false?

A

None

35
Q

What fungi causes penicilliosis?

a. Penicillium typhi
b. Candida tropicalis
c. Aspergillus fumigatus
d. Pneumocytosis merneffei

A

d

36
Q

What pneumocytosis has a characteristics of mold:

  • Green yellow colony
  • diffuse red pigment,
  • Septate
  • branching hyphae

a. P. carinii
b. P. jiroveci
c. P. merneffei

A

c

37
Q

In endemic mycoses, Males are more prominent than females

T or F

A

T

38
Q

2 fungi that can cause infection by spherules

a. Aspergillus and Conidia
b. Candida and Pneumocystitsis
c. Cryptococcus neoformans and gatti
d. Coccidioides immitis and Rhinosporidiosis

A

d

39
Q

A disease caused by Ascomycete which is known as San Joaquin Valley fever deu to airborne arthroconidia BSC

Also known as Desset Rheumatism

a. Coccidioidomycosis
b. Rhinospoidioidiosis
c. Aspergilloma

A

Coccidiodomycosis

40
Q

Antignic structure for Coccidioidomycosis that is an Ag preparation extracted from the filtrate of a liquid mycelial culture of C. immitis

a. Coccidioidin
b. spherulin

A

a

41
Q

Antignic structure for Coccidioidomycosis that produced from a filtrate of broth culture of spherules

a. Coccidioidin
b. spherulin

A

b

42
Q

Specimend used to diagnose Coccidioidin:

Sputum
Skin
CSF
Urine
Blood

Which of the following does not belong?

A

None

43
Q

KOH or Calcofluor white is used to detect Spherules

T or F

A

T

44
Q

Treatment for severe Coccidioidemycosis

a. Amphotericin B IV
b. Penicillin
c. Itraconazole
d. Oral fluconazole

A

a

45
Q

Most prevalent pulmonary fungal infection?

a. Mycoplasma
b. Histoplasma
c. Blastomycosis
d. Paracoccidioides

A

b

46
Q

Histoplasma can be seen in macrophages (Tissues)

T or F

A

T

47
Q

Antigenic structure of histoplasma that is mycelial broth culture

a. Histoplasmin
b. Polysacharide antigen

A

a

48
Q

Acute pulmonary histoplasmosis: male/Reactive/emphysema

Chronic pulmonary histoplasmosis: Immunocompetent

Severe Disseminated histoplasmosis: Immunosuppressd

T or F

A

F

Acute: Immunocompetent
Chronic: Males/Reative/Emphysema

49
Q

Fungi that has the shape of Broad base butt

a. Histoplasma
b. Blastomyces
c. paracoccidioides
d. coccidioides

A

b

50
Q

What is the specific antigen for blastomyces?

A

Antigen A

51
Q

In wet mount, this has the characteristics of broadly attached buds on thick-walled yeasts cells

a. Blastomyces
b. Histoplasma
c. Paracoccidioides
d. Coccidioides

A

b

52
Q

This fungal has the shape of mickey mouse ear

a. South American Blastomyces
b. Paracoccdioidomycosis brasiliensis

A

a

53
Q

This fungal has the shape of spokes of a ship’s steering wheel

a. South American Blastomyces
b. Paracoccdioidomycosis brasiliensis

A

b

54
Q

Specific antigen for paracoccidioides

A

Antigen P

55
Q

What test is used in detecting paracoccidioides?

a. Skin test
b. PCR
c. ELISA
e. Ab titer

A

a

56
Q

In paracoccidioides, what is being detected in skin test?

a. Paracoccidioidin
b. Antigen P
c. Paracoccidio enzyme
d. The fungi itself

A

a

57
Q

Which of the following fungi is nonculturable

  • Lobomycosis
  • Rhinosporidiosis
A

All

58
Q

In Hematoxylin and Eosin, All fungi shows:

Cytoplasm: Blue
Nuclei: Pink
Wall: white

T or F

A

F

Pink: cytoplasm
Blue: Nuclei
No color: wall

59
Q

Detects glycogen in tissues, fungal walls contain large amounts of glycogen

Blue nuclei

a. Periodic Acid - Schiff stain (PAS)
b. Gomori Methamine Silver stain
c. Mayer or Southgate Mucicarmine Stain
d. Fluorescent Antibody stain
e. Alcian stain
f. Gridley stain
g. Giemsa stain

A

a

60
Q

Stains mucopolysaccharides

a. Periodic Acid - Schiff stain (PAS)
b. Gomori Methamine Silver stain
c. Mayer or Southgate Mucicarmine Stain
d. Fluorescent Antibody stain
e. Alcian stain
f. Gridley stain
g. Giemsa stain

A

c

61
Q

Cryptococcus neoformans has a deep rose in what stain?

a. Periodic Acid - Schiff stain (PAS)
b. Gomori Methamine Silver stain
c. Mayer or Southgate Mucicarmine Stain
d. Fluorescent Antibody stain
e. Alcian stain
f. Gridley stain
g. Giemsa stain

A

c

62
Q

The internal parts of hyphae are deep rose to black and the background is light green

a. Periodic Acid - Schiff stain (PAS)
b. Gomori Methamine Silver stain
c. Mayer or Southgate Mucicarmine Stain
d. Fluorescent Antibody stain
e. Alcian stain
f. Gridley stain
g. Giemsa stain

A

b

63
Q

Stains the walls of most fungi
Background: Yellow
Tissue stain: Deep blue
Hyphae and yeast stain: Dark blue or rose

a. Periodic Acid - Schiff stain (PAS)
b. Gomori Methamine Silver stain
c. Mayer or Southgate Mucicarmine Stain
d. Fluorescent Antibody stain
e. Alcian stain
f. Gridley stain
g. Giemsa stain

A

f

64
Q

Useful to highlight the capsule of Cryptococcus which appear red or blue depending on the stain used

a. Periodic Acid - Schiff stain (PAS)
b. Gomori Methamine Silver stain
c. Mayer or Southgate Mucicarmine Stain
d. Fluorescent Antibody stain
e. Alcian stain
f. Gridley stain
g. Giemsa stain

A

e

65
Q

Simple, sensitive, and extremely specific method of detecting fungi tissues or fluids

a. Periodic Acid - Schiff stain (PAS)
b. Gomori Methamine Silver stain
c. Mayer or Southgate Mucicarmine Stain
d. Fluorescent Antibody stain
e. Alcian stain
f. Gridley stain
g. Giemsa stain

A

d

66
Q

Used for blood and bone marrow specimens

a. Periodic Acid - Schiff stain (PAS)
b. Gomori Methamine Silver stain
c. Mayer or Southgate Mucicarmine Stain
d. Fluorescent Antibody stain
e. Alcian stain
f. Gridley stain
g. Giemsa stain

A

g

67
Q

Goof initial differentiation of dimorphic fungi
Sputum

a. Gram stain
b. Brown and Brenn
c. India ink
d. Papanicolaou stain
e. latophenol cotton blue
f. Modified Acid Fast stain

A

d

68
Q

General stain similar to gram positive

a. Gram stain
b. Brown and Brenn
c. India ink
d. Papanicolaou stain
e. latophenol cotton blue
f. Modified Acid Fast stain

A

a

69
Q

Tissue Gram’s stain

a. Gram stain
b. Brown and Brenn
c. India ink
d. Papanicolaou stain
e. latophenol cotton blue
f. Modified Acid Fast stain

A

b

70
Q

Very popular for quick evaluation of fungal structures

Will stain the chitin in cell walls of fungi

a. Gram stain
b. Brown and Brenn
c. India ink
d. Papanicolaou stain
e. latophenol cotton blue
f. Modified Acid Fast stain

A

e

71
Q

Demonstrates the capsule of C. neoformans in CSF specimens
a. Gram stain
b. Brown and Brenn
c. India ink
d. Papanicolaou stain
e. latophenol cotton blue
f. Modified Acid Fast stain

A

c

Mycoviro (Finals) (8 decks)

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