32 - Bacteriophages Flashcards
1
Q
Give 4 reasons why the study of bacteriophages is important…
A
- Can turn bacteria virulent
- Outnumber all other organisms
- Potential alternative to antibiotics (phage therapy)
- Useful tool in labs
2
Q
Phages may have ssDNA, dsDNA or ssRNA genomes. Give examples of phages from each of these groups…
A
- ssDNA -> M13
- dsDNA -> T4, lambda, T3, T7
- ssRNA -> MS2
3
Q
The most common phage genomes are…
A
dsDNA
4
Q
ɸX174 is an…
A
Icosohedral phage
5
Q
M13 is a…
A
Filamentous phage
6
Q
Typical structure of a icosohedral phage…
A
Genetic material inside the ‘head’, collar where attached to tail, which ends in base plate and long tail fibres
7
Q
Name 5 uses of phages…
A
- Cloning (lambda, M13 and T7)
- Phage therapy
- ListShield for meat (stops lysteria)
- Phage display
- PRBP (phage receptor binding protein)
8
Q
Phage lambda is (lysogenic / lytic)
A
Phage lambda is lysogenic
9
Q
Lytic phage growth and replication can be examined using a…
A
Plaque assay (d’Herelle)
10
Q
A
