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Antimicrobial > 30s subunit > Flashcards

30s subunit Flashcards

1
Q 
Which of these antibiotics target 30s subunit?
A. Ketolides
B. Aminoglycosides
C. Tetracyclines
D. Spectinomycin
A

B,C, and D

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2
Q

Aminoglycosides can bind __________of the bacterial cell wall?

A

negatively charged lipopolysaccharide

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3
Q

What targets the A site with the 16s rRNA 30s subunit?

A

Aminoglycosides

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4
Q

What is the main target for the majority of aminoglycosides?

A

Internal loop Helix 44 of 16s rRNA, the P ste

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5
Q

Paromomycin fixes the flexible A1492 and A1493 in a conformation similar to induced fit by cognate aminoacyl-tRNA, this will do what to the protein?

A

Mutate the protein due to the fact that the 30s Subunit will close around a near cognate and result in the wrong amino acid.

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6
Q

Which aminoglycoside is said to be used to treat cystic fibrosis?

A

Gentamicin

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7
Q

Kanamycin and ___________ are prone to____________.

A

tobramycin; dimerization

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8
Q

Dimers cause inhibition of the 30s and 50s subunit interaction, which antibiotic duo does that?

A

Kanamycin and tobramycin

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9
Q 
Which stage of translation is the dimer interaction of kanamycin and tobramycin most likely to affect?
A. Elongation 1
B. Recycling 
C. Termination
D. Initiation 3
A

D. Initiation 3

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10
Q

Streptomycin binds close to which helix?

A

Helix 27

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11
Q

Streptomycin is the only aminoglycoside to possess which of these?

  1. streptindine back bone
  2. two sugar molecules
  3. a polar aryl
  4. None of the above.
A

A streptidine backbone

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12
Q

Ribosomal protein S12 is involved ?

A

with streptomycin, the formation of the closed structure 30s upon binding a cognate aminoacyl-tRNA

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13
Q

Streptomycin inhibits which stage of initiation?

A

Initiation II

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14
Q 
Which of these antibiotics prevent the normal dissociation of ribosomes?
A. Erythromycin
B. Tobramycin
C. Streptomycin
D. Kanamycin
A

C. Streptomycin

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15
Q

Which antibiotic is used to cure tuberculosis?

A

Streptomycin

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16
Q

What region of the 50s has been reported to interact with aminoglycosides?

A

Helix 69

17
Q

When H44 is already saturated, which region do aminoglycosides bind to?

A

Helix 69

18
Q

What antibiotics interact with H69 of the 50S resulting in inhibition of 70S dissociation.

A

Neomycin, gentamicin and paromomycin

19
Q

What explains the side effects of aminoglycosides.?

A

A1408

20
Q

T or F: Aminoglycosides are known to accumulate in the perilymph and endolymph of the inner ear and at high concentrations damage hair cells.

A

True

21
Q

Which antibiotic binds the 16S rRNA of the 30S in the helix 34 (H34)

A

Spectinomycin

22
Q

T and F: Spectinomycin binds to H34 and sterically blocks movement causing inhibition of translocation.

A

True

23
Q

T or F: Tetracycline binds at Tet-2 site on 16s rRNA

A

False; Tetracycline binds at Tet-1

24
Q

What does tetracycline prevent from occurring?
A. Prevent physical binding to P site
B. Prevent physical binding to A site
C . Prevent physical transfer of peptidyl bond
D. Prevent physical transfer of peptidyl bond to E site

A

B. Prevent physical binding to A site.

25
Q

Which of the 4 proteins does tetracycline interact with ?
A. S4 for Tet-2; S7 for Tet-6, S9 for Tet-4 and S17 for Tet-5
B. S1 for Tet-2; S7 for Tet-6, S9 for Tet-4 and S17 for Tet-5
C. S4 for Tet-2; S7 for Tet-6, S9 for Tet-4 and S10 for Tet-5
D. S4 for Tet-2; S10 for Tet-6, S9 for Tet-4 and S17 for Tet-5

A

A. S4 for Tet-2; S7 for Tet-6, S9 for Tet-4 and S17 for Tet-5

26
Q

T or F: All tetracycline binding sites could act synergistically to contribute to the strong bacteriostatic effect of tetracycline

A

True

27
Q

One of the most interesting forms of resistance to tetracyclines is?

A

Ribosomal protection:. It has been proposed that Tet-M binds to the A-site and actively releases tetracycline bound to it.

28
Q

What antibiotic is bacteriostatic at low concentrations and bactericidal at high concentrations

A

Mupirocin

29
Q

Isoleucine-tRNA-synthetase (IleRS)
is inactivated by which antibiotic?
Why?

A

Mupirocin; it mimics the IIeRS substrate

Antimicrobial (8 decks)

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