3 White Blood Cells Flashcards
T/F phagocytes are involved in wound healing?
True
What are the granulocytes?
The 3 “Phils.” (Baso, eosino, neutron)
What are the phagocytes?
Monocytes (and derivatives) plus the 3 “phils”
Neutrophil characteristics?
Short life, rapid movement
Eosinophil characteristics?
Usually in tissue, can phagocytose, more often use granules on parasites, allergens, tumors
Memory: “Ew I blew allergic snot on myself! Ew I have a parasite! Ew I have a tumor!”
Basophil characteristics?
Usually in tissue, release granules in immediate hypersensitivity rxns (heparin, histamine)
Monocyte characteristics?
Circulate briefly, move to tissue and live 2-3 months once specialized.
What is a rough normal white cell count?
3.7-10.5 K/mm3
What stimulates stem cells to become colony forming units?
IL-3
What stimulates colony forming units to proliferate, and to become granulocyte-monocyte colonies?
GM-CSF
What differentiates colony forming units to become granulocytes?
G-CSF (the others follow the same pattern, ex. monocyte-CSF. The exception is eosinophils)
What differentiates colony forming units to become eosinophils?
IL-5
Neutrophils take how long to make?
6-10 days (can be shorter under stress)
Which cells in stages of differentiation are capable of division? Which are not?
- Stem, progenitor, blasts, promyelocytes, myelocytes
- Metamyelocytes, band, PMN’s,
T/F the bone marrow does not contain mature neutrophils?
F. Contains 7x as many as the vascular pool
In blood, does the marginal or circulating pool have more PMN’s?
Same, unless they come out of marginal pool when under stress.
Where are marginal pool PMN’s hanging out?
Stuck to endothelium
How long does a PMN circulate b4 entering tissue?
7 hrs (then enter tissue or die)
What are contained in neutrophil granules?
elastase, defensins, cathelicidin (recall: kills gram+/- bacteria), lysozyme, lactoferrin
What are the stages of granulocyte differentiation?
myeloblast->
promyelocyte-> (1 or 2 immature “azurophilic” granules present)
myelocyte-> (color-specific granules appear)
metamyelocyte->
band->
1 of 3 “phil” cells
What takes place in endothelial activation prior to diapedesis? (NOT lymphocyte activation, which would activate the integrin on the lymphocyte)
-E then P selectin express at sites of inflammation
What is responsible for leukocyte rolling?
L-selectin (receptor on lymphocyte) binding carbohydrate lignands on endothelial cells
What causes firm leukocyte adherence to endothelium?
-Integrins on leukocyte get activated to adhere firmly to ICAM-1 (recall: CCR7 activated by CCL19 or CCL21)
What causes diapedesis?
CD31 or PECAM-1 (platelet endothelial cell adhesion molecule)
