3. Stem Cells and Regenerative Med Flashcards
Which of the following is true regarding stem cells?
a. Adult stem cells are pluripotent and can differentiate into any germ layer
b. Embryonic stem cells are found in the inner cell mass of the blastomere
c. Stem cells can be used in the treatment of sickle cell anemia
d. Stem cells of ectodermal origin can be used in treatment of joint diseases
C is correct
Which of the following is true regarding stem cells?
adult stem cells are multipotent and can differentiate into multiple but limited lineages
b. Embryonic stem cells are found in the inner cell mass of the BLASTOCYST
d. Stem cells of MESENCHYMAL origin can be used in tx of jt dz (such as adipose)
What causes teratomas in immunodeficient mice? A)Induced pluripotent stem cell B)Multipotent stem cells C)Bone marrow derived stem cells D)Embryonic stem cells
D)Embryonic stem cells
Hematopoeitic stem cells display what surface markers? a)CD 31 b)CD 34 c)PECAM d)LPS
b)CD 34
How are induced pluripotent stem cells created?
a) Using cell culture and adding growth factors
b) Use of high density and short duration UV light
c) Addition of base pairs to the nucleus of a differentiated cell
d) Using viral transduction of genes for nuclear programing
d)Using viral transduction of genes for nuclear programing
4) Adult stem cells are considered______?
a. Pluripotent
b. Multipotent
c. Unipotent
d. Differentiated cells only
b.Multipotent
Name the 4 types of stem cells
adult
embryonic
fetal/perinatal
induced pluripotent
Define pluripotent and name an example?
differentiate into cells from > 1 germ layer but NOT extraembryonic; (induced pluripotent stem cells, embryonic)
Define multipotent and name an example?
Restricted to the germ layer linage from which they originate; (fetal/perinatal; mesenchymal)
What are the 4 requirements for a cell to be called a mesenchymal stem cell?
Exhibit spindle cell shape
Form colonies of cells from single parent cells
Express a certain set of cell surface markers that exclude it from hematopoietic lineages
Possess tri-lineage differentiation
Osteoblast, Adipocyte, Chondrocyte
In the Black and colleagues study which evaluated the effect of a single injection of intra-articular SVF cells obtained from various locations in 18 dogs with osteoarthritis of the hip joint using a randomized, blinded, placebo-controlled, multicenter study. … clinical outcomes after therapy improved 30% to 40% compared to baseline.
true
Functional platelets are required for successful primary coagulation, or the complex process by which platelets adhere, spread, and aggregate to form a platelet plug that establishes a cell-matrix framework for initiation and completion of ______ hemostasis.
secondary
plasma sample to be considered platelet-rich plasma, the platelet concentration should be at least __-__ higher than the concentration in peripheral blood
1.5-2 b. 2-3 c. 3-5 d. 5-8
c 3-5
Platelet-rich plasma has been shown to increase the proliferation of mesenchymal stem cells and fibroblasts in a dose-dependent manner.
true
\_\_\_\_ present within platelet-rich plasma has been shown to increase collagen type I production in tendon fibroblasts TGF-β VEGF IGF PDGF
TGF-β
There is currently no evidence supporting the concept that platelet-rich plasma, if used alone, accelerates cortical bone healing.
True