3 Cells 2 Flashcards

1
Q

cyanobacterium

A
photosynth.. blue/green algae
produces O2:
-oceans from blue to green
-precipitation of aqueous iron
-killed most life on earth: great oxygenation event 2.3 bill yrs ago
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2
Q

primordial earth

A

anerobic and very acidic

there are still some niches like that

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3
Q

layered rocks in Austrilia

A

layers of RUST
due to changing oxygen levels
begining of movement to life on land started with the atmosphere started getting oxygen

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4
Q

Layered rocks theory 1

A

O2 production caused ecological catastrophe, ecosystem collapsed and it took a long time for it to recover and for cyannobacteria to produce enough oxygen again
it was a SLOW event

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5
Q

Layered Rock Theory 2

A

Global Ice Ball Theory
O2 oxidizes of methane
lots of bacteria produce methane as a byproduct
there was lots of methane in atmosphere before cyannobacteria (so earth was very warm)
cyannobacteria introduces oxygen: planet freezes over
thaw again, oxidation, methane causes warming, etc

lots of freezing and thawing

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6
Q

pores and transport systems

A

active/passive

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7
Q

secretion systems

A

send complexs/proteins beyond the membrane

  • pili:transmission of DNA
  • flagella: motillity
  • toxin secretion: assembly of things outside of the cell
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8
Q

goal of cells:

A

use nrg to do work outside of cell
ATP… you can’t send it outside of the membrane
isntead charge and transport things out to do work

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9
Q

Active vs. passive transport

A

whether based on conc gradient or ATP use

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10
Q

passive transport

A

movement of small molecs

based on conc gradient

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11
Q

aquaporin

A

water only

passive, based on gradeint

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12
Q

nonspecific transporters

A

anything goes back and forth

passive, based on gradeint

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13
Q

specific transporters

A

use no nrg, but use only specific things

passive, based on gradeint

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14
Q

facillitated diffusion

A

uses channels and carrier proteins

specific, passive, CAN BE SATURATED

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15
Q

osmosis

A

water transport
based on salt concs on either side of cell
isotonic, hypotonic, hypertonic

remember, water can move across membrane, not salt

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16
Q

isotonic

A

concs of salt and water on same on inside and outside of cell
net 0 diffusion
there is movement of water molecs, but not NET change
very good for cell

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17
Q

hypertonic

A

more water inside cell, more salt outside
water moves out
cell shrinks, not lethal though

18
Q

hypotonic

A

more salt inside cell

swelling and lysing for cells with weak walls because water rushes in

19
Q

how to avoid lysing of bacteria in hypotonic solution?

A

cell wall!!!
peptidoglycan layer is a lattice that prevents bursting
resistant to hypo and hyper

20
Q

how do BODY CELLS deal with gram positive bacteria

A

surround it with a ton of water (create hypotonic solution around the bacteria cell)
break cell wall with enzymes
lysing bc no more cell wall

21
Q

how we use hypertonic solutions

A

pickle juice
salting meat
does not sterilize!!!!! bacteria dont spoil the food because we slow them down, but theyre not dead

22
Q

active transport

A

use energy to transport sugars, amino acids, organic acids, inorganic ions

ususally involves binding of things like ATP and the target to the transport protein, hydrolysis of ATP, conf. changes

23
Q

bacterial secretion

A

of a sticky sugar called glycocalyx

24
Q

glycocalyx

A

defense: avoid dehydration (survie dessication: immediate deydradtion)
slime layer on bacterial surface. stifky
made inside of cell and secreted out

slime layer of capsule

25
function of glycocalyx
attachment to surfaces... aid in colonization protect from desiccation/dehydration nutrient trap virulance factor: protects from phagocyotisis from immune cell - if immunse cell grabs it, it can't hold on
26
capsule vs. slime layer
capsule is more organized and better attached
27
S layer
on surface of gram positive bacteria inert, sit. protection and adhesion purifying them makes them self assmble into strucutre that perfectly fits around the bacteria. wound spring... have a little nrg that makes them self assemble
28
spore
way for bacteria to survive in exterme environemnts preserve genetic material kind of secreted MOSTLY GRAM POSITIVE (a few gram negative have it too)
29
spores are resistant to...
``` heat radiation toxins antibiotics dessication ```
30
net number of cells with endospore formation
is zero | mother cell dis
31
what do spores look like in cells?
light, nonstaining areas within cells
32
what kills spores???
Bleach!!!!!!!!!
33
formation of endospores
assymetric division engulf the smaller cell (both contain genetic material) mother dies: so NOT a replication event (no net gain of cells) genome equivalent: not number of cells, number of genomes
34
Quarum Sensing
communication and determination of local concs of same and other species of bacteria
35
Autioinducer systems and quarum sensing
secrete small diffusible compounds bacteria react to high concs of chems and alter their gene expressions atp to make light
36
light organ squid
vibrio fisheri: produce visible light at high bacteria concs in Squid have it in bellies contract light organ to make more light bellies so that predators cant see them when they pass over moon and create shadow squid produces sugar so that the bacteria grows in its light organ
37
how do bacteria know concentration
quarum sensing decsion making based on concs: inter and intra species sense conc of metabolic byproducts (waste) thus they know how many other bacteria are around can direct them how fast to eat/metabolize
38
Biofilms: quarum sensing
``` protect from immune system, dislodging organized group of bacteria matrix of bacteria and glycocalyx. functions like multicellular organism inc anitbioitc resistance plaque on teeth ``` make descsions based on conc. from free living bacteria to adhesive form.
39
3 things protein complexes do
secretion adhesion motility
40
central dogma
DNA-> RNA-> proteins->work! | solved by Crick
41
S (spedbergs)
rate of sedimentation affected by density, size, and shape measures the particle moving through a thick solution the two RNA subunits are more effective when the move together, thats why the number is smaller