2.6.1 Speacial Topic Flashcards

1
Q

Swabs are recommended for filamentous fungi

T or F

A

F

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2
Q

Fungi Culture specimen should be handled in a class ____ BSC in BSL _____ or ____

A

II

II, III

For H. capsulatum, C. immitis, B. dermatitidis

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3
Q

General rule transport at (Temp?)_____________ preferably within ___ hours

A

Room Temp

2

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4
Q

Delay specimen Temp:

____ for non sterile

____ for Sterile

A

4C

37C

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5
Q

Skins, hair and nails can be delayed overnight

T or F

A

T

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6
Q

Specimen for hair:

Clipping or plucking from the base of hair shaft

T or F

A

T

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7
Q

Specimen for hair:

Collect from areas of scaling or alopecia

T or F

A

T

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8
Q

Specimen for hair:

Result of Microsporum in Wood lamp’s

A

(+) except M. gypseum

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9
Q

Specimen for hair:

Result of Micrsporum in Hair infection

A

Ectothrix

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10
Q

Specimen for hair:

Result of Trichophyton in Wood lamp’s

A

(-)

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11
Q

Specimen for hair:

Result of Trichophyton in Hair infection

A

Endothrix

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12
Q

Skin and nails is disinfect with?

A

70% alcohol

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13
Q

Specimen for Skin:

Scraped the skin from the ______ of the surface lesion

A

Edge

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14
Q

Specimen for Skin:

Scalp hair is brush with sterile?

A

Toothbrush or hairbrush

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15
Q

Direct examination for Skin?

A

10% KOH

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16
Q

Specimen for Nails:

Scraped underneath the nail plate and surface; Clippings

T or F

A

T

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17
Q

What is the Direct examination for Nail?

A

KOH mount

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18
Q

Specimen for Tissue Biopsies:

Tissue should be ground, not minced

T or F

A

F

Minced, not ground

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19
Q

What fungi is an exception for grounding tissue biopsy

A

Histoplasma

20
Q

What specimen is obtained through exudate using needle and syringe?

A

Abscess and subcutaneous infections

21
Q

Biopsy can be performed if using aspiration is not recovered for abscess and subcutaneous infection

T or F

22
Q

Respiratory specimen:

Sputum is collected at what time?

A

Early morning

23
Q

Sputum = Deep coughh

T or F

24
Q

If the sputum is too viscous, what is being added?

A

NALC (Liquefying agent)

25
Bronchoscopy is done in which specimen?
Respiratory specimen
26
What is the broth for blood culture?
BHI
27
Isolator tube is done on _____ centrifugation for blood spceimen
Lysis
28
Isolator tube contains?
Saponin, PEG, anticoagulant
29
Isolator tube is recommended especially for what fungi?
Histoplasma
30
Biphasic media for blood specimen T or F
T
31
Automated method such as BACTEC is done especially for M. furfur T or F
F Not for M.furfur
32
Blood is incubated at 30C for 21 days T or F
T
33
Filamentous fungi rarely grows on blood except for?
Fusarium (Sickal shaped)
34
Which part of centrifuged blood is taken for Histoplasma?
Buffy coat
35
Yeast is usally seen in Blood T or F
T (BT)
36
Anticoagulant for Bone marrow?
Heparin (Heparinized)
37
Anticoagulant is for what fungi?
Histoplasma
38
Bedside inoculation for Bonemarrow T or F
T
39
CSF is centrifuge at ________ rpm for _____ mins prior to examination
1500-2000, 20 mins
40
Direct examination of india ink is used for what fungi?
Cryptococcus neoformans (Encapsulated Yeast)
41
Eye specimen is collected through Corneal scrapings; eye discharge T or F
T
42
Urine sample Is collected at _________ for around 10mL
1st early morning
43
Urine is centrifuge prior to inoculation T or F
T
44
Urine is for recovery of yeast specifically?
Candida
45
Vaginal specimen is transported within _____ hrs for (Culture transport swabs
24