2.6.1 Speacial Topic Flashcards
Swabs are recommended for filamentous fungi
T or F
F
Fungi Culture specimen should be handled in a class ____ BSC in BSL _____ or ____
II
II, III
For H. capsulatum, C. immitis, B. dermatitidis
General rule transport at (Temp?)_____________ preferably within ___ hours
Room Temp
2
Delay specimen Temp:
____ for non sterile
____ for Sterile
4C
37C
Skins, hair and nails can be delayed overnight
T or F
T
Specimen for hair:
Clipping or plucking from the base of hair shaft
T or F
T
Specimen for hair:
Collect from areas of scaling or alopecia
T or F
T
Specimen for hair:
Result of Microsporum in Wood lamp’s
(+) except M. gypseum
Specimen for hair:
Result of Micrsporum in Hair infection
Ectothrix
Specimen for hair:
Result of Trichophyton in Wood lamp’s
(-)
Specimen for hair:
Result of Trichophyton in Hair infection
Endothrix
Skin and nails is disinfect with?
70% alcohol
Specimen for Skin:
Scraped the skin from the ______ of the surface lesion
Edge
Specimen for Skin:
Scalp hair is brush with sterile?
Toothbrush or hairbrush
Direct examination for Skin?
10% KOH
Specimen for Nails:
Scraped underneath the nail plate and surface; Clippings
T or F
T
What is the Direct examination for Nail?
KOH mount
Specimen for Tissue Biopsies:
Tissue should be ground, not minced
T or F
F
Minced, not ground
What fungi is an exception for grounding tissue biopsy
Histoplasma
What specimen is obtained through exudate using needle and syringe?
Abscess and subcutaneous infections
Biopsy can be performed if using aspiration is not recovered for abscess and subcutaneous infection
T or F
T
Respiratory specimen:
Sputum is collected at what time?
Early morning
Sputum = Deep coughh
T or F
T
If the sputum is too viscous, what is being added?
NALC (Liquefying agent)
Bronchoscopy is done in which specimen?
Respiratory specimen
What is the broth for blood culture?
BHI
Isolator tube is done on _____ centrifugation for blood spceimen
Lysis
Isolator tube contains?
Saponin, PEG, anticoagulant
Isolator tube is recommended especially for what fungi?
Histoplasma
Biphasic media for blood specimen
T or F
T
Automated method such as BACTEC is done especially for M. furfur
T or F
F
Not for M.furfur
Blood is incubated at 30C for 21 days
T or F
T
Filamentous fungi rarely grows on blood except for?
Fusarium (Sickal shaped)
Which part of centrifuged blood is taken for Histoplasma?
Buffy coat
Yeast is usally seen in Blood
T or F
T (BT)
Anticoagulant for Bone marrow?
Heparin (Heparinized)
Anticoagulant is for what fungi?
Histoplasma
Bedside inoculation for Bonemarrow
T or F
T
CSF is centrifuge at ________ rpm for _____ mins prior to examination
1500-2000, 20 mins
Direct examination of india ink is used for what fungi?
Cryptococcus neoformans (Encapsulated Yeast)
Eye specimen is collected through Corneal scrapings; eye discharge
T or F
T
Urine sample Is collected at _________ for around 10mL
1st early morning
Urine is centrifuge prior to inoculation
T or F
T
Urine is for recovery of yeast specifically?
Candida
Vaginal specimen is transported within _____ hrs for (Culture transport swabs
24
