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MTAP 1 Myco > 2.6.1 Speacial Topic > Flashcards

2.6.1 Speacial Topic Flashcards

1
Q

Swabs are recommended for filamentous fungi

T or F

A

F

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2
Q

Fungi Culture specimen should be handled in a class ____ BSC in BSL _____ or ____

A

II

II, III

For H. capsulatum, C. immitis, B. dermatitidis

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3
Q

General rule transport at (Temp?)_____________ preferably within ___ hours

A

Room Temp

2

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4
Q

Delay specimen Temp:

____ for non sterile

____ for Sterile

A

4C

37C

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5
Q

Skins, hair and nails can be delayed overnight

T or F

A

T

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6
Q

Specimen for hair:

Clipping or plucking from the base of hair shaft

T or F

A

T

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7
Q

Specimen for hair:

Collect from areas of scaling or alopecia

T or F

A

T

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8
Q

Specimen for hair:

Result of Microsporum in Wood lamp’s

A

(+) except M. gypseum

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9
Q

Specimen for hair:

Result of Micrsporum in Hair infection

A

Ectothrix

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10
Q

Specimen for hair:

Result of Trichophyton in Wood lamp’s

A

(-)

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11
Q

Specimen for hair:

Result of Trichophyton in Hair infection

A

Endothrix

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12
Q

Skin and nails is disinfect with?

A

70% alcohol

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13
Q

Specimen for Skin:

Scraped the skin from the ______ of the surface lesion

A

Edge

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14
Q

Specimen for Skin:

Scalp hair is brush with sterile?

A

Toothbrush or hairbrush

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15
Q

Direct examination for Skin?

A

10% KOH

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16
Q

Specimen for Nails:

Scraped underneath the nail plate and surface; Clippings

T or F

A

T

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17
Q

What is the Direct examination for Nail?

A

KOH mount

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18
Q

Specimen for Tissue Biopsies:

Tissue should be ground, not minced

T or F

A

F

Minced, not ground

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19
Q

What fungi is an exception for grounding tissue biopsy

A

Histoplasma

20
Q

What specimen is obtained through exudate using needle and syringe?

A

Abscess and subcutaneous infections

21
Q

Biopsy can be performed if using aspiration is not recovered for abscess and subcutaneous infection

T or F

A

T

22
Q

Respiratory specimen:

Sputum is collected at what time?

A

Early morning

23
Q

Sputum = Deep coughh

T or F

A

T

24
Q

If the sputum is too viscous, what is being added?

A

NALC (Liquefying agent)

25
Q

Bronchoscopy is done in which specimen?

A

Respiratory specimen

26
Q

What is the broth for blood culture?

A

BHI

27
Q

Isolator tube is done on _____ centrifugation for blood spceimen

A

Lysis

28
Q

Isolator tube contains?

A

Saponin, PEG, anticoagulant

29
Q

Isolator tube is recommended especially for what fungi?

A

Histoplasma

30
Q

Biphasic media for blood specimen

T or F

A

T

31
Q

Automated method such as BACTEC is done especially for M. furfur

T or F

A

F

Not for M.furfur

32
Q

Blood is incubated at 30C for 21 days

T or F

A

T

33
Q

Filamentous fungi rarely grows on blood except for?

A

Fusarium (Sickal shaped)

34
Q

Which part of centrifuged blood is taken for Histoplasma?

A

Buffy coat

35
Q

Yeast is usally seen in Blood

T or F

A

T (BT)

36
Q

Anticoagulant for Bone marrow?

A

Heparin (Heparinized)

37
Q

Anticoagulant is for what fungi?

A

Histoplasma

38
Q

Bedside inoculation for Bonemarrow

T or F

A

T

39
Q

CSF is centrifuge at ________ rpm for _____ mins prior to examination

A

1500-2000, 20 mins

40
Q

Direct examination of india ink is used for what fungi?

A

Cryptococcus neoformans (Encapsulated Yeast)

41
Q

Eye specimen is collected through Corneal scrapings; eye discharge

T or F

A

T

42
Q

Urine sample Is collected at _________ for around 10mL

A

1st early morning

43
Q

Urine is centrifuge prior to inoculation

T or F

A

T

44
Q

Urine is for recovery of yeast specifically?

A

Candida

45
Q

Vaginal specimen is transported within _____ hrs for (Culture transport swabs

A

24

MTAP 1 Myco (34 decks)

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