1.6.2 Laboratory Diagnosis of Fungal diseases (Culture) Flashcards
General purpose culture medium for fungi
Sabouraud Dextrose agar
pH for SDA?
5.6
In mycosel or mycobiotic, the SDA contains?
a. Cycloheximide
b. Cycloheximide with Methylene blue
c. Cycloheximide with chloramphenicol
d. Cycloheximide with 10% KOH
c
Used to diff of T. rubru and T. mentagrophytes
a. Dermatophyte test medium
b. Cornmeal agar with 1% glucose
c. Sabouraud Brain Heart Infusion
d. Urease Test medium
e. Cottonseed medium
f. Cornmeal agar with tween 80
d
Medium for Blastomyces
a. Dermatophyte test medium
b. Cornmeal agar with 1% glucose
c. Sabouraud Brain Heart Infusion
d. Urease Test medium
e. Cottonseed medium
f. Cornmeal agar with tween 80
e
Allows growth of dermatophytes and to separate saprophytes and bacteria
a. Dermatophyte test medium
b. Cornmeal agar with 1% glucose
c. Sabouraud Brain Heart Infusion
d. Urease Test medium
e. Cottonseed medium
f. Cornmeal agar with tween 80
a
pH indicator for Dermatophyte test
Phenol red
Dermatophytes color in Dermatophyte test
Red
Used for Trichophyton rubrum and T. mentagrophytes; chlamydospore production
a. Dermatophyte test medium
b. Cornmeal agar with 1% glucose
c. Sabouraud Brain Heart Infusion
d. Urease Test medium
e. Cottonseed medium
f. Cornmeal agar with tween 80
b
Presumtive ID of Candida?
a. Dermatophyte test medium
b. Cornmeal agar with 1% glucose
c. Sabouraud Brain Heart Infusion
d. Urease Test medium
e. Cottonseed medium
f. Cornmeal agar with tween 80
f
Czapeck’s agar is used for?
a. Aspergillus
b. Rhizopus
c. Cryptococcus
a
Birdseed agar is used for?
a. Aspergillus
b. Rhizopus
c. Cryptococcus
Cryptococcus
In Trychophyton agar, it contains _____ casein,_______ ammonium nitrates
1-5, 6-7
Incubation temp for Fungi medium?
RT
Cutlure held for _____ days before reporting as Negative
30
