2.2.2 Cell culture for Virus

Question 1

MRC5

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 1

b

Question 2

Human Embryonic kidney

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 2

a

Question 3

Hela

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 3

c

Question 4

WI-38

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 4

b

Question 5

Rabbit Kidney

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 5

a

Question 6

Primary Monkey kidney

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 6

a

Question 7

Hep-2

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 7

c

Question 8

KB

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 8

c

Question 9

A549

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 9

c

Question 10

Vero Cell

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 10

c

Question 11

LLC-MK2, MDCK

a. Primary cell
b. Diploid Cells
c. Immortal cells

Answer 11

c

Question 12

DNA virus grows better at ______ While RNA viru grows better in_____

Answer 12

MRC5, RMK

Question 13

Incubation for cell cultures?

Answer 13

35-37

Question 14

Incubation for Respiratory viruses?

Answer 14

33C

Question 15

Duration for incubation of VIrus?

Answer 15

1-4 weeks (2 weeks)

Question 16

Example of Fast growers (1-3 days)

a. HSV
b. VZV
c. CMV
d. Influenza

Answer 16

a

Question 17

Moderate growers duration?

Answer 17

2-10 days

Question 18

Slow growers duration?

Answer 18

7-14 days

Question 19

Slow growers example

a. HSV
b. VZV
c. CMV
d. Influenza

Answer 19

b and c

Question 20

Cytopathic Effect (CPE) grading

1+ 1-25 of monolayer
2+ 26-50
3+ 51-75
4+ 76-100

Which among is significant?

Answer 20

2+ and above

Question 21

For influenza viruses, PIV, measles, mumps, rubella

a. Hemadsorption
b. Plaque formation

Answer 21

a

Question 22

Clear area on a lawn of cell cultures or bacteria where viruses destroyed the cells, cluster of necrotic bodies

a. Hemadsorption
b. Plaque formation

Answer 22

b

Question 23

For viral it is usually expresed in PFU/mL which is 10% of infectious dose

T or F

Answer 23

F

50%

Question 24

Shell vial cultures are faster in detecting viral antigens using fluorescein-labeled antibodies

T or F

Answer 24

T

Question 25

Rapid modidication of convntional cell cultures?

a. Viral quantification
b. Shell vial cultures

Answer 25

b