2.2.2 Cell culture for Virus Flashcards
MRC5
a. Primary cell
b. Diploid Cells
c. Immortal cells
b
Human Embryonic kidney
a. Primary cell
b. Diploid Cells
c. Immortal cells
a
Hela
a. Primary cell
b. Diploid Cells
c. Immortal cells
c
WI-38
a. Primary cell
b. Diploid Cells
c. Immortal cells
b
Rabbit Kidney
a. Primary cell
b. Diploid Cells
c. Immortal cells
a
Primary Monkey kidney
a. Primary cell
b. Diploid Cells
c. Immortal cells
a
Hep-2
a. Primary cell
b. Diploid Cells
c. Immortal cells
c
KB
a. Primary cell
b. Diploid Cells
c. Immortal cells
c
A549
a. Primary cell
b. Diploid Cells
c. Immortal cells
c
Vero Cell
a. Primary cell
b. Diploid Cells
c. Immortal cells
c
LLC-MK2, MDCK
a. Primary cell
b. Diploid Cells
c. Immortal cells
c
DNA virus grows better at ______ While RNA viru grows better in_____
MRC5, RMK
Incubation for cell cultures?
35-37
Incubation for Respiratory viruses?
33C
Duration for incubation of VIrus?
1-4 weeks (2 weeks)
Example of Fast growers (1-3 days)
a. HSV
b. VZV
c. CMV
d. Influenza
a
Moderate growers duration?
2-10 days
Slow growers duration?
7-14 days
Slow growers example
a. HSV
b. VZV
c. CMV
d. Influenza
b and c
Cytopathic Effect (CPE) grading
1+ 1-25 of monolayer
2+ 26-50
3+ 51-75
4+ 76-100
Which among is significant?
2+ and above
For influenza viruses, PIV, measles, mumps, rubella
a. Hemadsorption
b. Plaque formation
a
Clear area on a lawn of cell cultures or bacteria where viruses destroyed the cells, cluster of necrotic bodies
a. Hemadsorption
b. Plaque formation
b
For viral it is usually expresed in PFU/mL which is 10% of infectious dose
T or F
F
50%
Shell vial cultures are faster in detecting viral antigens using fluorescein-labeled antibodies
T or F
T
Rapid modidication of convntional cell cultures?
a. Viral quantification
b. Shell vial cultures
b
