2.2.1 Lab diagnosis (Microscopy, Viral antigen detection) Flashcards
This type of microscope uses negative stain which provides a detention limit >10^6 to 10^7 mL; useful for nonculturable viruses
Electron Microscope
Light microscope can be used to see cytopathic effects of Viruses
T or F
T
Lipschultz Body, Henderson Patterson Bodies
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola
d
Round, Swollen Refractile cells, occasionally Syncytia
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola
b
Tzanck smear Detects Multinucleated Giant Cells in
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola
b
Discrete foci of rounded, swollen, refractile cells, Slowly involves entire monolayer
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola
c
MULTINUCLEATED GIANT CELL
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola
b
Smudge cells
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola
a
Rounding and aggregation of infected cells in GRAPE LIKE
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola
a
Decoy cells in Urine Specimen
a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus
e
Owl’s Eye nuclear inclusion
a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus
a
Bizzare astrocytes, Oligodendrocyte nuclei enlarged foci of demyelination in white matte
a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus
d
Koilocytes
a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus
b
Enlarged group glass nuclei with chromatin precipitation in erythroid precursors
a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus
c
Negri Bodies
a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps
b
Characteristic refractile angular or tear shaped CPE; progress to involve entire
a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps
a
Synctia formation
a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps
e
Destructive degeneration with swollen, Vacuolated cells
a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps
d
Flower Nuclei in T cells
a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps
c
Syncytia in Hep-2
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever
b
Characteristic refractile rounding cells
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever
c
Warthin Finkeldey cells
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever
d
Councilman bodies
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever
e
CPE is usually absent or minimal
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever
a
DFA and IFA uses fluorescence labeling, Which of the following is significant?
a. (-) No apple green Fluorescence
b. 1+ Fairly yet unequivocal apple green Fluorescence
c. 2+ apple green Fluorescence
d. 3+ Bright apple green Fluorescence
e. 4+ Brilliant apple green Fluorescence
c, d, e
Enzyme linked Virus inducible System (ELVIS) is used for HSV
T or F
T
Gold standard for Viral Diagnosis
a. Molecular methods
b. Microscopy
c. Cell culture
d. Serology
c
Cell culture contains cell monolayer and cell culture medium
T or F
T
What is the pH indicator for Cell culture medium?
Phenol red
