2.6: Environmental Control of Metabolism Flashcards

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1
Q

What are the main micro-organisms?

A

Bacteria
Archaea
Eukaryotes

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2
Q

Why are micro-organisms used?

A

They are adaptable
Easy to cultivate
Grow quickly

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3
Q

What do micro-organisms use and produce?

A

They use a wide variety of substrates for metabolism

They produce a range of products from their metabolic pathways

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4
Q

Give examples of complex molecules required for biosynthesis.

A

Amino acids
Vitamins
Fatty acids

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5
Q

What do micro-organisms require?

A

Raw materials

Energy source

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6
Q

Give examples of complex molecule reactions

A

Amino acids –> protein
Sugars –> carbohydrates (starch, cellulose, glycogen)
Fatty acids + glycerol –> fats/lipids

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7
Q

Where is an energy source derived from?

A

Chemical substrates

Light in photosynthesis

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8
Q

What do sterile conditions do?

A

Reduce competition with desired micro-organisms for nutrients
Reduce the risk of spoilage of the product

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9
Q

What are the culture conditions?

A

Sterility
Control of temperature
Oxygen levels
pH

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10
Q

What are the phases of a bacteria growth chart thing

A

Lag phase
Log/exponential phase
Stationary phase
Death phase

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11
Q

Explain the Lag phase

A

Where enzymes are induced (give rise to) to metabolise substrates

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12
Q

Explain the Log/exponential phase

A

Contains the most rapid growth of micro-organisms due to plentiful nutrients

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13
Q

Explain the stationary phase

A

Occurs due to the nutrients in the culture media becoming depleted (used up) and the production of toxic metabolites

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14
Q

Explain the death phase

A

Occurs due to toxic accumulation of metabolites or the lack of nutrients in the culture

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15
Q

When are secondary metabolites produced

A

In the stationary phase

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16
Q

Why are secondary metabolites better in the wild

A

They have an ecological advantage by allowing the micro-organisms to outcompete other micro-organisms

17
Q

Define viable cell count

A

Counting only the living organisms

18
Q

Define total cell count

A

Cell numbers decrease

19
Q

What can micro-organisms be gown on?

A

Soild media e.g nutrients agar

Liquids e.g nutrient broth

20
Q

What equipment is used in the industry?

A

Fermenters

21
Q

What graph is used for interpreting growth curves?

A

Semi-logarithmic graphs