2.3 Nucleic acids Flashcards
What is the monomer for DNA?
Nucleotides
What is the structure of a nucleotide for DNA?
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Phosphate group (-) Pentose sugar (Deoxyribose) [Contains H] Nitrogenous base
What is the structure of a nucleotide for RNA?
Phosphate group (-) Pentose sugar (Ribose) [Contains OH hydroxyl group] Nitrogenous base
What are the different types of nitrogenous bases?
-Purines = double ring structures
(adenine and guanine)
-Pyrimidines = single ring structures
(thymine, cytosine and uracil)
How are nucleotides formed and broken?
-Formed: Condensation reaction between the phosphate and the sugar & the sugar and the base
This would produce two water molecules
-Broken: Hydrolysis reaction between the phosphate and the sugar & the sugar and the base
This would use up two water molecules
What is the structure of DNA?
- Sugar phosphate backbone
- Nitrogenous base pairs join to make the rungs
- Held together by weak hydrogen bonds
What are the base pairs?
- A purine always binds to a pyrimidine so that the rungs of the ladder are always the same length:
- Adenine joins to thymine
- Cytosine joins to guanine
How are the base pairs joined?
- Two hydrogen bonds between A & T (or U)
- Three hydrogen bonds between C & G
How would you describe the way that the strands of the DNA run?
- The two strands run in an anti parallel fashion (5-3 on one side and 3-5 on the other side)
- The two strands are also twisted into a double helix
What is the structure of DNA? (9)
- DNA is a polymer composed of many repeating subunits (monomers) called nucleotides
- Each nucleotide is made up of a pentose sugar, a phosphate group and a nitrogenous base
- These three molecules form nucleotides via two condensation reactions producing two molecules of water
- In the DNA molecule there are two strands that run in an antiparallel fashion due to the reversal of the direction of the deoxyribose sugar
- The sugar phosphate forms the backbone of the DNA
- The sugar and phosphate groups are held together by strong covalent phosphodiester bonds
- The nitrogenous bases on each strand are bonded with weak hydrogen bonds in the middle of the DNA, according to their complimentary base pairings (A&T with 2 hydrogen bonds and C&G with three hydrogen bonds)
- As the bases always bond to complimentary pairing it means that when the DNA replicates, the sequence on one strand can be used to determine the sequence of bases on the other strand due to the complimentary base pairing
- A pyrimidine will always bind to a purine to ensure the same length on each of the rungs
- The DNA strands are also twisted to form a double helix structure
What are the steps of DNA replication?
1) DNA starts as a double helix
2) DNA gyrase untwists the helix and DNA helicase unzips the helix, breaking the hydrogen bonds
[Single stranded binding proteins prevent reannealing of the strands after helicase unzips the helix]
3) DNA polymerase binds to the leading strand and moves along it, this helps form the covalent/ phosphodiester bonds between the free DNA nucleotides and the strand
[The DNA polymerase moves in the 5’-3’ direction]
4) On the lagging strand the DNA polymerase binds and forms the covalent bonds from the 5’-3’ but going in the opposite direction
7) DNA ligase closes the gaps where DNA polymerase cannot bind this is because ligase can build from 3’-5’
8) DNA replication is completed semi conservatively as each new molecule of DNA has one old and one new strand
How does the Mesleson and Stahl experiment support the idea of semi conservative replication for DNA?
The DNA was made up of an equal mixture of N14 and N15, this was because the two new N14 strands joined each strand, so they are made of one N14 and one N15
What is transcription?
- When DNA turns into mRNA
- Occurs in the nucleus
- Involving the enzyme: RNA polymerase
What is the process of transcription?
The process begins when a gene is triggered to be used, this causes transcription factors to bind to the gene
1) A gene is unzipped by helicase, breaking the hydrogen bonds
2) RNA polymerase binds to the gene to then catalyse the temporary binding of free nucleotides to the template strand
3) This produces a mRNA strand that is complimentary to the template strand and the same as the coding strand but T’s are replaced with U’s
4) The mRNA then leaves the nucleus via the nuclear pores to then attach to ribosomes
What is the process of translation?
1) The mRNA binds to a ribosome via the small subunit, then the large binds
2) 2 amino acids come in and joins the tRNA
3) The anticodon on the tRNA is complimentary to the codon on the mRNA, temporary hydrogen bonds then form between the two
4) Peptide bonds between the amino acid and the tRNA’s are transferred to the two amino acids, this forms the peptide bond between the two amino acids on the tRNA’s
5) The ribosomes move along and a new complimentary tRNA comes in, peptide bonds continue to be formed between each amino acid, forming a sequence of amino acid as the ribosome moves along and new tRNA’s come in
6) A polypeptide is formed from the sequence of amino acids
7) The polypeptide finishes forming and the amino acid sequence stop when a stop codon is hit, this is because there is no complimentary anticodon on a tRNA for the stop codon to temporarily bind to, to create more amino acids so the amino acid sequence stops
8) mRNA moves away from the ribosome and the ribosome has finished the process of translating
8) The polypeptide is formed, then secondary and tertiary structure is then formed to give 3D structure of the protein