2. MICROPIPETTING AND THE USE OF PH METER - LAB Flashcards
What are tools used to measure extremely small volumes of liquid?
Micropipettes
What defines the accuracy of a micropipette?
Defined range of volume
What is the principle of operation for micropipettes?
Air displacement
What is the smallest volume a P10 micropipette can aspirate?
0.2 µL
What is the volume range for a P10 micropipette?
0.5-10 µL
What is the volume range for a P20 micropipette?
2-20 µL
What is the volume range for a P200 micropipette?
20-200 µL
What is the volume range for a P1000 micropipette?
200-1000 µL
Parts of Micropipettes
PUSH BUTTON
TIP EJECTOR BUTTON
TIP EJECTOR COLLAR
VOLUMETER
What part is used for aspiration or dispensing the fluid?
Push button
What part removes the micropipette tip after use?
Tip ejector button
What part pushes down the micropipette tip?
Tip ejector collar
Where are the dial windows or numbers located?
Volumeter
What instrument is used to measure the pH of a solution?
pH meter
What are the two types of measuring probes in a pH meter?
Glass electrode and reference electrode
What is measured by a pH meter?
Concentration of hydrogen ions
What is the standard buffer used in molecular biology for pH 7.0 (±0.02)?
Buffer solution pH 7.0
What is the primary use of a buffer solution in pH meters?
Calibration buffer
What types of buffer solutions are used for pH calibration?
Neutral pH 7, Acidic pH 4, Alkaline pH 10
How does a pH meter function?
Measures voltage between two electrodes
