2. MICROPIPETTING AND THE USE OF PH METER - LAB

Question 1

What are tools used to measure extremely small volumes of liquid?

Answer 1

Micropipettes

Question 2

What defines the accuracy of a micropipette?

Answer 2

Defined range of volume

Question 3

What is the principle of operation for micropipettes?

Answer 3

Air displacement

Question 4

What is the smallest volume a P10 micropipette can aspirate?

Answer 4

0.2 µL

Question 5

What is the volume range for a P10 micropipette?

Answer 5

0.5-10 µL

Question 6

What is the volume range for a P20 micropipette?

Answer 6

2-20 µL

Question 7

What is the volume range for a P200 micropipette?

Answer 7

20-200 µL

Question 8

What is the volume range for a P1000 micropipette?

Answer 8

200-1000 µL

Question 9

Parts of Micropipettes

Answer 9

PUSH BUTTON
TIP EJECTOR BUTTON
TIP EJECTOR COLLAR
VOLUMETER

Question 10

What part is used for aspiration or dispensing the fluid?

Answer 10

Push button

Question 11

What part removes the micropipette tip after use?

Answer 11

Tip ejector button

Question 12

What part pushes down the micropipette tip?

Answer 12

Tip ejector collar

Question 13

Where are the dial windows or numbers located?

Answer 13

Volumeter

Question 14

What instrument is used to measure the pH of a solution?

Answer 14

pH meter

Question 15

What are the two types of measuring probes in a pH meter?

Answer 15

Glass electrode and reference electrode

Question 16

What is measured by a pH meter?

Answer 16

Concentration of hydrogen ions

Question 17

What is the standard buffer used in molecular biology for pH 7.0 (±0.02)?

Answer 17

Buffer solution pH 7.0

Question 18

What is the primary use of a buffer solution in pH meters?

Answer 18

Calibration buffer

Question 19

What types of buffer solutions are used for pH calibration?

Answer 19

Neutral pH 7, Acidic pH 4, Alkaline pH 10

Question 20

How does a pH meter function?

Answer 20

Measures voltage between two electrodes