1. LABORATORY Flashcards
Specialized in DNA cloning and recombinant DNA technology
Cloning lab
Handle different cell lines
Diff type of cells
Diff cell assays
Cell culture laboratory
basta RNA
RNA lab
Study makes use of yeasts as a model organism (laboratory)
Yeast laboratory
Bacteria processing experiments
Bacteria laboratory
Large animals, basta animals experiments
Animal laboratory
Limits what you can and cannot do in a laboratory
States which organisms or viruses could be handled
Take into account the safety of the personnel
It changes the rules and equipment and samples in the laboratory
Biosafety level
General laboratory biosafety level
BSL 1
Handles infectious microbes biosafety level
BSL 2
Higher level of containment biosafety level
BSL 3 and 4
The neatest type of laboratory in any institute
For general use of everyone
Bates College General Equipment Laboratory
Largest general laboratory for molecular biology
National Institute of Molecular Biology and Biotechnology - UP DILIMAN
Ewan basta lab siya example na may sink daw
Dougherty Laboratory at Caltech
Has reverse microscope
University of Copenhagen - GLOBE Institute
Tip boxes daw
Molecular Immunology Laboratory at Sabanci Universitesi
Universal precaution
All specimens treated as pathologic
Should have plastic caps and contains reagents in different volumes
Should have label
Reagent bottles
Mixing reagents equipment
Erlenmeyer flask and beakers
Measurement of larger volumes
Graduated cylinders
Used for corrosive agents for stocked solutions pipet
Glass pipettes
Pipet used in serology used to transfer small volumes
Serological pipettes
Used in cell culture
Pasteur Pipette/Plug Pasteur Pipette
of different sizes
used for containing material to be centrifuged
Centrifuge tube
Smaller centrifuge tubes
Microcentrifuge tubes
instead of using small tubes one by
one, we have this strip so you won’t lose all.
Be careful when taking off the cap.
Tube strip for PCR
it can be a six wall plate, twelve
wall plate, etc.
Plates for different assays
this is what you put on a micropipette or
pipettor
Pipette tips
used for PCR (esp. for Quantitative PCR)
since we deal a lot of samples for that kind of experiment.
Sometimes you would consume all 96 tubes in one run
Tube Rack
for different uses not only for culturing as it can also be used for staining
Dishes
-80°C freezer
used for Pfizer vaccine
Ultra low freezer
Prize of ultra low freezer
700k or more
Preferred basic refrigerator
Two door refrigerator
depends on enzyme using,
what temperature do they work best
Reaction Temperatures
for cultures, what temperature do they grow (usually at 37°C, but sometimes at 40°C or above or sometimes lower depending on the experiment)
Incubation Temperatures
depending on where you are, the temperature can range from 24°C or if from the Philippines, it can be from 35°C, 36°C, 37°C
Ambient/RT (Room Temp)
Where to store certain materials requiring certain temperatures
4°C (Refrigerator temperature),
-20°C (Freezer temperature(refrigerator))
-60°C (Different kind of refrigerator)
-80°C (Ultra low freezer), in liquid nitrogen
Pipette useful for cell culture
Electronic pipette
1,000 microliter color of tip pipettor
Blue tip
200 microliter color of tip pipettor
yellow tip
10 microliter or smaller color of tip pipettor
white tip
Pipettor that has multiple tips
Multichannel pipettor
Multichannel pipettor is important in what types of experiments
Enzyme Linked Immunosorbent Assay
Used for measuring
weights of reagents
hydroscopic reagents
analytical balance
pH Meter
Top loading balance
Important in dealing with reagents that are effervescent, if they produce fumes
Fume hood
Workspace especially when doing cultures of microbes
Class 2 cabinet which means that the filtered air leaving it contains no pathogen
Top part of such has filter system preventing any particulates, any pathogens from leaving
Biosafety cabinet
Creates class 1 and class 2 water depending on what you need
It can distil or ionize it depending on the
experiment you need it for
Filtration system
+ Can be any storage container
+ This is where we store the water that has been
treated
Storage Container Jack
Biosafety cabinets uses what filters
HEPA filter
high efficiency particulate air
Sterilizing equipment
Standing autoclave
Drying oven
Sterilizing by wet heat
Standing autoclaveS
Standing autoclaves are giant pressure cookers that sterilize materials by wet heat at _______ temperature and ____________ pressure for ____________ minutes
121 degrees Celsius
15 pounds per square inch
15-20 minutes
Sterilization by dry heat
Drying oven
Drying oven temp
300 degrees Celsius for a long period of time
Think of it as high tech flame test
Measure the amount of ions that you have depending on the flame produced
Very complicated and expensive machine
Atomic Absorption Spectrophotometer
use a light source to illuminate a sample with light across the UV to the visible wavelength range (typically 190 to 900 nm).
The instruments then measure the light absorbed, transmitted, or reflected by the sample at each wavelength
UV-visible (UV-Vis) spectrophotometers
Incubators
Shaking Microbial Incubator
Cell Culture Incubator
Any laboratory needs this especially if doing cloning work since a lot of the cloning work use E. coli. Whether grown on a plate or liquid
medium, needs an incubator to grow
Microbial Incubator
for cell culture if growing eukaryotic cells needs them to be in condition wherein there is a continuous carbon dioxide in the atmosphere
Carbon Dioxide Incubator
For microcentrifuge tubes
For spinning down reagents
Tabletop centrifuge
For conical tubes
Refrigerated centrifuge
Used for washing blots, gels, membranes and different staining specimens
Orbital Shaker
Some reagents require heating and stirring for a very long time, that is why we use this machine
Hot Plate with Stirrer
Such little knob at the top vibrates at a high speed so that when you put a microcentrifuge tube beside it, vibrations agitates the contents of the tube and the mixes it
Vortex mixer
For tissue homogenization
Releases high frequency sound waves in order to create vibrations
Sonicator Setup
Used to dissolve agarose in the buffer and used to make the gel. Important in any electrophoresis laboratory
Microwave
Apparatus for Agarose Gel Electrophoresis
Tap
Power Supply
Protein Electrophoresis Unit
Imaging System
In Agarose Gel Electrophoresis, this is where the gel is placed
Tap
Such creates the cathode and anode and that DNA molecule would move towards the postive
Power Supply
Two parts of the Protein Electrophoresis Unit
Vertical Unit - samples go down
Power Supply
Either gel or blot, it is able to capture pictures
Imaging system
Used for transferring DNA or RNA in a gel
Transfer
If there’s a gel being stained, simply put it here to dry
Drying apparatus
a device used to test and study the degradation of materials which are subjected to continuous cycling between hot and cold temperatures during their service life.
Thermocycler
