1. LABORATORY Flashcards by 횐호 강

Specialized in DNA cloning and recombinant DNA technology

Cloning lab

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Handle different cell lines
Diff type of cells
Diff cell assays

Cell culture laboratory

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basta RNA

RNA lab

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Study makes use of yeasts as a model organism (laboratory)

Yeast laboratory

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Bacteria processing experiments

Bacteria laboratory

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Large animals, basta animals experiments

Animal laboratory

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Limits what you can and cannot do in a laboratory

States which organisms or viruses could be handled

Take into account the safety of the personnel

It changes the rules and equipment and samples in the laboratory

Biosafety level

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General laboratory biosafety level

BSL 1

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Handles infectious microbes biosafety level

BSL 2

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Higher level of containment biosafety level

BSL 3 and 4

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The neatest type of laboratory in any institute

For general use of everyone

Bates College General Equipment Laboratory

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Largest general laboratory for molecular biology

National Institute of Molecular Biology and Biotechnology - UP DILIMAN

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Ewan basta lab siya example na may sink daw

Dougherty Laboratory at Caltech

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Has reverse microscope

University of Copenhagen - GLOBE Institute

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Tip boxes daw

Molecular Immunology Laboratory at Sabanci Universitesi

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Universal precaution

All specimens treated as pathologic

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Should have plastic caps and contains reagents in different volumes

Should have label

Reagent bottles

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Mixing reagents equipment

Erlenmeyer flask and beakers

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Measurement of larger volumes

Graduated cylinders

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Used for corrosive agents for stocked solutions pipet

Glass pipettes

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Pipet used in serology used to transfer small volumes

Serological pipettes

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Used in cell culture

Pasteur Pipette/Plug Pasteur Pipette

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of different sizes

used for containing material to be centrifuged

Centrifuge tube

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Smaller centrifuge tubes

Microcentrifuge tubes

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instead of using small tubes one by one, we have this strip so you won't lose all. Be careful when taking off the cap.

Tube strip for PCR

it can be a six wall plate, twelve wall plate, etc.

Plates for different assays

this is what you put on a micropipette or pipettor

Pipette tips

used for PCR (esp. for Quantitative PCR) since we deal a lot of samples for that kind of experiment. Sometimes you would consume all 96 tubes in one run

Tube Rack

for different uses not only for culturing as it can also be used for staining

Dishes

-80°C freezer used for Pfizer vaccine

Ultra low freezer

Prize of ultra low freezer

700k or more

Preferred basic refrigerator

Two door refrigerator

depends on enzyme using, what temperature do they work best

Reaction Temperatures

for cultures, what temperature do they grow (usually at 37°C, but sometimes at 40°C or above or sometimes lower depending on the experiment)

Incubation Temperatures

depending on where you are, the temperature can range from 24°C or if from the Philippines, it can be from 35°C, 36°C, 37°C

Ambient/RT (Room Temp)

Where to store certain materials requiring certain temperatures

4°C (Refrigerator temperature), -20°C (Freezer temperature(refrigerator)) -60°C (Different kind of refrigerator) -80°C (Ultra low freezer), in liquid nitrogen

Pipette useful for cell culture

Electronic pipette

1,000 microliter color of tip pipettor

Blue tip

200 microliter color of tip pipettor

yellow tip

10 microliter or smaller color of tip pipettor

white tip

Pipettor that has multiple tips

Multichannel pipettor

Multichannel pipettor is important in what types of experiments

Enzyme Linked Immunosorbent Assay

Used for measuring weights of reagents hydroscopic reagents analytical balance pH Meter

Top loading balance

Important in dealing with reagents that are effervescent, if they produce fumes

Fume hood

Workspace especially when doing cultures of microbes Class 2 cabinet which means that the filtered air leaving it contains no pathogen Top part of such has filter system preventing any particulates, any pathogens from leaving

Biosafety cabinet

Creates class 1 and class 2 water depending on what you need It can distil or ionize it depending on the experiment you need it for

Filtration system

+ Can be any storage container + This is where we store the water that has been treated

Storage Container Jack

Biosafety cabinets uses what filters

HEPA filter high efficiency particulate air

Sterilizing equipment

Standing autoclave Drying oven

Sterilizing by wet heat

Standing autoclaveS

Standing autoclaves are giant pressure cookers that sterilize materials by wet heat at _______ temperature and ____________ pressure for ____________ minutes

121 degrees Celsius 15 pounds per square inch 15-20 minutes

Sterilization by dry heat

Drying oven

Drying oven temp

300 degrees Celsius for a long period of time

Think of it as high tech flame test Measure the amount of ions that you have depending on the flame produced Very complicated and expensive machine

Atomic Absorption Spectrophotometer

use a light source to illuminate a sample with light across the UV to the visible wavelength range (typically 190 to 900 nm). The instruments then measure the light absorbed, transmitted, or reflected by the sample at each wavelength

UV-visible (UV-Vis) spectrophotometers

Incubators

Shaking Microbial Incubator Cell Culture Incubator

Any laboratory needs this especially if doing cloning work since a lot of the cloning work use E. coli. Whether grown on a plate or liquid medium, needs an incubator to grow

Microbial Incubator

for cell culture if growing eukaryotic cells needs them to be in condition wherein there is a continuous carbon dioxide in the atmosphere

Carbon Dioxide Incubator

For microcentrifuge tubes For spinning down reagents

Tabletop centrifuge

For conical tubes

Refrigerated centrifuge

Used for washing blots, gels, membranes and different staining specimens

Orbital Shaker

Some reagents require heating and stirring for a very long time, that is why we use this machine

Hot Plate with Stirrer

Such little knob at the top vibrates at a high speed so that when you put a microcentrifuge tube beside it, vibrations agitates the contents of the tube and the mixes it

Vortex mixer

For tissue homogenization Releases high frequency sound waves in order to create vibrations

Sonicator Setup

Used to dissolve agarose in the buffer and used to make the gel. Important in any electrophoresis laboratory

Microwave

Apparatus for Agarose Gel Electrophoresis

Tap Power Supply Protein Electrophoresis Unit Imaging System

In Agarose Gel Electrophoresis, this is where the gel is placed

Tap

Such creates the cathode and anode and that DNA molecule would move towards the postive

Power Supply

Two parts of the Protein Electrophoresis Unit

Vertical Unit - samples go down Power Supply

Either gel or blot, it is able to capture pictures

Imaging system

Used for transferring DNA or RNA in a gel

Transfer

If there's a gel being stained, simply put it here to dry

Drying apparatus

a device used to test and study the degradation of materials which are subjected to continuous cycling between hot and cold temperatures during their service life.

Thermocycler