2/20: Corynebacterium, Listeria, Bacillus, Mycoplasma, Myobacteria Flashcards
Cornebacterium diptheria general info
gram positive, aerobic
Pathogenic cornebacterium diptheria resides on _____
oropharynx
Pathogenic cornebacterium diptheria produces ____
diptheria toxin encoded on lysogenic bacteriophage
Non-pathogenic form of cornebacterium diptheria is called ___
these inhabit ____
diptheroids
These inhabit pharynx, nasopharynx, distal urethra, skin
Diptheria
caused by local (like _____) effects of diptheria toxin
and by systemic (like ____) effects of diptheria toxin
local - severe pharyngitis or tonsilitis
systemic - circulation of toxin ib lood can cause acute myocarditis
The toxin in cornebacterium diptheria is type ___ toxin
it is ___ endotoxin.
Describe the roles of the alpha and beta subunits.
type II toxin
a-b endotoxin (2 protein complex)
beta subunit binds to cell, endocytosis brings into cell
low pH of endocytic vacuole causes unfolding
alpha subunit translocated to the cytoplasm and inhibits protein synthesis
Cornebacterium diptheria is spread via ___
droplets, direct contact, fomites (less often)
T/F: Cornebacterium is found regularly in US
false
less than 10 cases per year because we use VACCINE
Describe the diagnosis of cornebacterium diptheria
clinically, then grown on selective media
Describe the immune response to cornebacterium diptheria
the toxin is antigenic
stimulates production of antibodies during inrection
What is the inactivated form of cornebacterium diptheria toxin used as a vaccine?
What is the immune response to this?
formalin = inactivated toxin used as vaccine
this is still antigenic - stimulates antibody production
Describe the prevention of diptheria
Immunizations provide protection against infections
Done in 3-4 shots in the first years of life, boosters every 10 years
Describe the treatment of diptheria
early administration of antitoxin, pencicllin, cephalosporin, erythromycin, tetracycline
Describe listeria monocytogenes general info
gram positive, bacilli (can resemble cornyebacterium and streptococci), aerobic (distinguishes it from strep)
How do we distinguish listeria monocytogenes from cornebacterium diptheria in terms of BACTERIOLOGY?
listeria monocytogenes = tumbling motility on fluid media at templs below 30* C, grows well on rich media at temps as low as 0* C
Listeria monocytogenes is the only listeria species that is _____ in humans
pathogenic
Listeriosis usually only presents itself when there is ____
widespread infection
associated w/ listeria monocytogenes
Foodborne outbreaks of listeriosis, symptoms = _____
nausea, stomach pain, diarrhea
widespread infection of listeriosis symptoms - ______
fever, malaise, occasional bacteremia
can cause encephalitis ad meningitis
What are the 3 main virulence factors of listeria monocytogenes?
- internalin
- lysteriolysin O
- actin polymerization
Why is internalin is a major virulence factor for listeria monocytogenes?
internalin attach to the host cells so that the bacteria can be taken up by endocytosis
Why is lysteriolysin O a major virulence factor for listeria monocytogenes?
lysteriolysin O lyses the endocytic vacuole and the bacteria is spread to the cytoplasm to replicate
Why is actin polymerization a major virulence factor for listeria monocytogenes?
actin polymerization allows it to properl itself from cytoplasm to the neighboring cell
Where is listeria monocytogenes found?
ubiquitous to soil, water, GI tracts of animals
foodborn pathogens spread from deli meat, dairy, uncooked food stored at low temps
Why is listeria monocytoenes hard to eliminate?
because of formation of biolfilm
Who is most susceptible to infection of listeria monocytogenes?
infants and elderly
How do we diagnose infection of listeria monocytogenes?
blood/CSSF culture which would show beta-hemolytic gram positive rods