19.4

Question 1

genome analysis

Answer 1

-focuses on identifying the roles/purpose of DNA elements in the genome
*comparative genomics
*functional genomics
*proteomics

Question 2

comparative genomics reveals conserves regions

Answer 2

-uses information from one genome to learn about a second, related genome
-based on synteny

Question 3

synteny

Answer 3

conserved arrangement of DNA in related genomes
-can predict gene function

Question 4

functional genomics reveals function

Answer 4

uses biotechnology to highlight the connection between genotype and phenotype
1. study all the RNA molecules produced by the genome: transcriptome
2. study all proteins produced by genome: proteome
3. study the interactions between proteins

Question 5

study of the transcriptome

Answer 5

*DNA microarrays
*RNA-seq
* just because an mRNA is transcribed doesn’t mean the protein product will be translated

Question 6

DNA microarrays

Answer 6

-which genes are being expressed in a particular location or time
-provide insight into the function of genes
-must create microarray chip with “known”

Question 7

RNA-seq

Answer 7

-use next gen sequencing technology
-capture all the mRNA created

Question 8

proteomics

Answer 8

study of the proteome or collection of proteins encoded in the genome
-more difficult to analyze than the transcriptome
*mass spectromety
*protein microarrays

Question 9

mass spectrometry (mass spec)

Answer 9

uses charge to mass ratio to identify protein or component amino acids

Question 10

protein microarrays

Answer 10

rely on protein-specific antibodies to identify proteins translated

Question 11

predict protein structure

Answer 11

bioinformatics: uses computer programing mathematics and modeling to analyze data
-can be used to predict protein structure

Question 12

application of genomics

Answer 12

-synthetic biology : create organisms
-diagnostics: medicine/forensics
-ethical concerns: gene patenting