1.4 Proteins Flashcards

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1
Q

Give examples of different functions of proteins within an organism

A

The proteins within an organism function as
- Enzymes
- Hormones
- Receptors
- Antibodies
- Channel-forming proteins
- Carrier proteins

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2
Q

State the definition of an enzyme

A

An enzyme is a biological catalyst. Enzymes are found inside all living cells where they speed up chemical reactions while remaining unchanged themselves in the process

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3
Q

Define the terms ‘substrate’ and ‘product’ in an enzyme reaction

A

Substrate- Chemical substance at the start of a chemical reaction also known as the raw materials in the reaction
Product- Chemical substance made at the end of a chemical reaction
ENZYME
Substrate————- Product

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4
Q

Identify the type of chemical the enzymes are made of

A

Enzymes are made of protein

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5
Q

Define the phrase ‘active site of an enzyme’

A

The ‘active site’ of an enzyme is the region of the enzyme which is a specific shape to be complementary to the enzymes specific substrate. This shape means that the enzyme and its specific substrate will fit perfectly together during the reaction

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6
Q

Define the phrase ‘ Enzyme-substrate complex’

A

As a product of ‘ active site of an enzyme’ as soon as the reaction has taken place the substrate will be replaced by product and the complex breaks up

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7
Q

Explain the meaning of the term ‘ complementary’ in terms of enzyme and substrate

A

Complementary is a term that refers to the corresponding shapes of the enzymes active site and its specific substate that allow them to fit together. The active site and the substrate fit together like pieces of a jigsaw puzzle

  • DONOT use the term matching
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8
Q

Explain the term specificity in terms of enzymes and substrate

A

Each enzyme is a specific to a particular shape. This mean that the active site of one enzyme only corresponds to the shape of one particular substrate. E.G. Catalase can only work on hydrogen peroxide, amylase only works on starch ect

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9
Q

Identify two types of enzyme reactions

A

Enzyme reactions can be categorised as either synthesis or degradation reactions. Synthesis reactions build up small substrates into larger products. Degradation reactions break down large substrates into smaller products

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10
Q

Give examples of degradation and synthesis enzyme reactions, identifying the substrate, enzyme and product(s)

A
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11
Q

Explain the term ‘Optimum’ as applied to a range of conditions on which enzymes operate

A

Optimum is a term used to refer to the conditions in which an enzyme will be most active i.e. conditions where the enzyme will work at its fastest rate. The two main conditions that affect enzyme activity are temperature and PH

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12
Q

Describe the effect temperature and PH on the rate of an enzyme-controlled reaction if its at its optimum

A

If the temperature and PH are optimum for an enzyme-controlled reaction it will be working at its fastest rate.

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13
Q

Describe the effect temperature and PH have on the rate of an enzyme-controlled reaction if its not at its optimum

A

If the temperature or PH are decreased below optimum or increased above optimum then this will reduce the enzyme activity and the rate of the reaction will decrease

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14
Q

Explain what happens to an enzyme when its denatured

A

When an enzyme has been denatured the shape of its active site has been changed. The rate of the reaction controlled by the enzyme will decrease. Enzymes and other proteins can be denatured by changed away from optimum PH and by temperatures above optimum temperature

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15
Q

Explain why a denatured enzyme is no longer functional

A

When an enzyme has been denatured the shape of the enzymes active site is no longer complementary to its substrate. This means that the enzyme can no longer operate because its active site no longer fits the shape of its specific substrate

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16
Q

Describe how to measure enzyme activity

A

Enzyme activity can be measured by collecting the product of the reaction over a period of time e.g. total volume of oxygen gas released in one minute during HPCOW reaction.