1.4 Detecting Proteins Using Antibodies Flashcards
1
Q
What are immunoassay techniques used for?
A
To detect and identify specific proteins
2
Q
What are monoclonal antibodies?
A
Antibodies with the same specificity
3
Q
What type of antibodies do immunoassay techniques use?
A
Monoclonal antibodies
4
Q
What is a chemical label?
A
The ‘label’ is often a reporter enzyme producing a colour change, but chemiluminescence, fluorescence and other reporters can be used.
5
Q
What can antibodies be used to detect?
A
Both the presence and concentration of a specific protein within a solution.
6
Q
When is Western Blotting used?
A
After SDS- PAGE electrophoresis
