14 Flashcards
demonstrated bacterial transformation
Frederick Griffith
DNA fragments were taken up by bacteria and changed their
phenotyp
Frederick Griffith
worked with bacteriophages
Alfred Hershey and Martha Chase
viruses ntroduced their DNA into the bacterial host, not their protein.
Isotopes were used to label the molecules
Alfred Hershey and Martha Chase
analyzed nitrogenous bases by species
Erwin Chargaff’s
Who found out that different species had different ratios of base pairs
Erwin Chargaff’s
used X-ray crystallography to see the actual molecule
Maurice Wilkins and Rosalind Franklin
Analyses of DNA composition lead
to what became known as
Chargaff’s Rules
are credited with finally solving the puzzle as
to the actual structure of the molecule.
James Watson and Francis Crick
Short segments of nucleotides are polymerized
in the 5’ to 3’ direction
– away from the fork
Okazaki fragments
Okazaki fragments oare covalently bonded together by the enzyme
Dna Ligase
Inserts the RNA primer
sequence
Primase
lagging strand is made in short fragments called
Okasaki fragments
Okazaki fragments are connected by
Ligase