1.3 Flashcards

1
Q

Taxonomy

A

Science of classifying/ naming biological organisms

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2
Q

Phylogeny

A

Study of evolutionary relationships between organisms

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3
Q

what do taxonomists use to make classifications

A

genotypes, phenotypes, phylogenetic info

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4
Q

what have taxonomists been using to guide groupings for microbes

A

increasingly used phylogeny and used phenotype less

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5
Q

Order of hierarchial groups

A

domain
kindom
phylum
class
order
family
genus
species

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6
Q

what groups do bacteria not really use

A

kingdom, use eubacteria

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7
Q

what did carl linnaeus do

A

made a system of classification with the 2 names: genus and species

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8
Q

what was system of classification for

A

minimize chaos, define structure to be used when new species are discovered

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9
Q

what us the purpose of scientific names

A

describe characteristics
honour a scientist
describe physical properties/ appearance

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10
Q

subspecies

A

finer classification after species

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11
Q

biovar or biotype

A

physiological biochemically distinct from other members of species

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12
Q

serovar or serotype

A

based on surface antigens

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13
Q

example of organism whos name based on an important feature

A

STEG ( shiga toxin producing e. coli)

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14
Q

strain

A

specific isolate of a genetic variant or subtype

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15
Q

what is O157: H7

A

a serotype of ecoli that gives chickenpox

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16
Q

what does taxonomy do for us

A

brings order to chaos
give mfs the means to make prediction about a organisms characteristics

17
Q

phylogenic trees

A

predicted evolutionary relationships

18
Q

phylogenic tree time

A

left(root) to right(modern)

19
Q

phylogenic tree branches

A

show evolutionary history unique to connecting lineages

20
Q

phylogenic trees branch length

A

show evolutionary distance between nodes

21
Q

what DNA sequences are taxonomists looking for

A

highly conserved genes with highly conserved functions that accumulate mutations slowly over time

22
Q

what is a conserved feature of all organisms on earth

23
Q

what sequence is commonly sequenced to infer phylogenic relationship

A

rRNA of the SSU of the ribosome

24
Q

rRNA

A

ribosoml RNA

25
SSU
small subunit
26
what are variable regions vs conserved regions of SSU rRNA useful for?
variable regions useful for identifying relationships conserved useful for PCR
27
how is 16S rDNA used to identify and classify bacteria
1) isolate genomic DNA 2) PCR primer used to bind conserved regions of 16S rDNA 3) PCR amplify and sequence 16S DNA, 4)align and analyze sequences
28
how can gene sequencing via PCR be made better
compare sequences of multiple conserved genes
29
Limitations of phylogenic trees
-they are predictions: can only infer them, dont know them -HGT confuses things: foreign DNA may be mixed with ancestral -Aquired DNA may undergo homolohous recombination: replaced with homologous genes of another organism -selective pressure: DNA mutates at different rates in different species
30
HGT
horizontal gene transfer