1.2 Replication Of DNA

Question 1

What type of bonds must break before DNA can be replicated?

Answer 1

Hydrogen bonds between bases

Question 2

What is a primer?

Answer 2

A short strand of nucleotides that bind to the 3’ prime end of the template strand

Question 3

Describe how new nucleotides are added

Answer 3

DNA polymerase adds DNA nucleotides using complementary base pairing to the 3’ prime ends of new DNA strand

Question 4

Which direction is DNA replicated?

Answer 4

5’ to 3’

Question 5

What enzyme controls sugar phosphate bonding?

Answer 5

DNA polymerase

Question 6

What enzyme binds the DNA fragments together?

Answer 6

Ligase

Question 7

What starand is replicated in fragments?

Answer 7

The lagging strand

Question 8

To which end can DNA polymerase add nucleotides?

Answer 8

3’ end

Question 9

State the three requirements for DNA replication

Answer 9

DNA
Primers 
Nucleotides 
ATP
Enzymes

Question 10

What dose PCR do to DNA?

Answer 10

PRC amplifies the DNA

Question 11

What is the purpose of heatinf the DNA in PRC

Answer 11

Separates the strands or denatures the DNA

Question 12

What is the purpose of reducing the heat after hearing it during a PCR

Answer 12

Allows the primer to bond

Question 13

State the use of PCR

Answer 13

solve crimes, paternity tests, diagnose genetic disorders