11. Transport of oxygen and carbon dioxide in the blood Flashcards
Structure of Hb
4 polypeptide chains
2 x α and 2 x β chains
Each chain assoc with a haem group
(contains a ferrous ion; Fe2+)
Function: oxygen ‘carrier’
Each haem can bind 1 O2 molecule
Each Hb molecule therefore carries 4 O2 molecules
INCREASED affinity for O2:
change in Oxygen dissociation curve
Left shift
DECREASED affinity for O2:
change in Oxygen dissociation curve
Right shift
Special adaptations of Fetal Haemoglobin (HbF)
Different polypeptide chains in the global:
α2γ2 (causes a left shift)
O2 affinity higher with HbF
HbF will pick up O2 from maternal HbA at a given PO2
Gradual replacement of HbF with HbA after birth (months)
Advantage:
easier release of O2 to tissues
Less tissue hypoxia
Persistence of HbF
Thalassaemia
Tx: blood transfusions
Physiological changes that cause a Rightward shift (reduced affinity)
Increased temperature
Decreased pH (protonation; Bohr effect)
Increased CO2 levels (direct and via H+)
Increased 2,3-diphosphoglycerate (2,3-DPG)
CO2 Modes of transport
Solution in water = 10%
Carbamino compounds = 30%
Bicarbonate (HCO3-) = 60%
From CO2 to HCO3-
CO2 enters the RBC by diffusion down a partial pressure gradient
Carbonic anhydrase (CA) converts CO2 to carbonic acid
H2CO3 rapidly dissociates to hydrogen ions and bicarbonate ions
Bicarbonate is transported out of the RBC
Chloride shift
H+ binds to oxyHb and lowers its affinity for O2… O2 is delivered to the tissues (Bohr effect)
Increased ability of deoxygenated blood to carry CO2 (Haldane Effect)