10 Molecular Genetics

Question 1

Hershey-Chase experiment had radioactively-labeled protein, the isotope was

Answer 1

35S

Question 2

Hershey-Chase experiment had radioactively-labeled DNA. Protein was

Answer 2

32P

Question 3

Who discovered the double helix?

Answer 3

Rosalind Franklin!

Question 4

DNA nucleotide base pair bonding:
The pair that has a double bond is _ and _.
The pair that has a triple bond is _ and _.

Answer 4

Double: T and A.
Triple: G and C.

Question 5

Which base pairs are stronger, C-G or A-T?

Answer 5

C-G

Question 6

DNA: Bond between sugar and phosphate. Is it covalent or ionic, intermolecular or intramolecular?

Answer 6

Covalent intramolecular.

Question 7

DNA nucleotide bases: What’s a single-ring structure called?

Answer 7

Pyrimidine

Question 8

DNA nucleotide bases: What’s the larger, double-ring structure called?

Answer 8

Purine

Question 9

Examples of pyrimidines?

Answer 9

Thymine and cytosine

Question 10

Examples of purines?

Answer 10

Adenine and guanine

Question 11

What bond joins the NUCLEOTIDES in DNA?

Answer 11

Covalent. (Remember that the nucleotide is the entire subunit of the polynucleotide/DNA strand. It includes the deoxyribose sugar, the phosphate group, and the nitrogenous base.)

Question 12

DNA nucleotides: what kind of bonding occurs between nitrogenous bases?

Answer 12

Hydrogen bonding.

Question 13

Semiconservative model

Answer 13

During dna replication, parental molecule‘s strands split apart, free nucleotides complete them, making two identical daughter strands. Daughter strands are half from parental molecule, half of strand is “Conserved” in daughter strand.

Question 14

Explain promoter in RNA nucleotide synthesis

Answer 14

Promoter: a nucleotide sequence that is a binding site for sigma factor (protein) and RNA polymerase.

Question 15

What is the first phase of transcription

Answer 15

Initiation. Attachment of RNA polymerase to the promoter.