[1] LESSON 6: URINALYSIS—MICROSCOPIC EXAMINATION OF URINE Flashcards
May indicate the presence of certain sediments
Color
Pathologic or non-pathologic causes of turbidity
Clarity
Blood
RBCs, RBC Cast
Protein
Casts, cells
Nitrite
Bacteria, WBCs
LE
WBCs, WBC Casts, bacteria
Visualization of elements with low refractive indices
Phase-Contrast Microscopy
Phase-Contrast Microscopy elements
hyaline, mixed cellular cast, MT, Trichomonas
Interference-Contrast Microscopy Types
A. Nomarski (Differential)
B. Hoffmann (Modulation)
Bright-Field microscopes can be adapted
Interference-Contrast Microscopy
3-D microscopy image and layer-by-layer imaging of a specimen
Interference-Contrast Microscopy
SternheimerMalbin components
(CV + Safranin O)
Enhances nuclear detail
0.5% Toluidine Blue
% Acetic acid
2%
Hansel Stain components
Eosin Y + Methylene blue
Stains TAG and neutral fats (orangered)
Lipid stains
Lipid stains components
ORO and Sudan III
Urinary eosinophils
Hansel Stain
Crystals (normal) Moderate
5-20
Bacteria
Rare
Bacteria few
10-50
Casts Quantitated
Per LPF
RBCs
Per HPF
WBCs Quantitated
Per HPF