[1] LESSON 6: URINALYSIS—MICROSCOPIC EXAMINATION OF URINE Flashcards

1
Q

May indicate the presence of certain sediments

A

Color

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2
Q

Pathologic or non-pathologic causes of turbidity

A

Clarity

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3
Q

Blood

A

RBCs, RBC Cast

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4
Q

Protein

A

Casts, cells

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5
Q

Nitrite

A

Bacteria, WBCs

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6
Q

LE

A

WBCs, WBC Casts, bacteria

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7
Q

Visualization of elements with low refractive indices

A

Phase-Contrast Microscopy

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8
Q

Phase-Contrast Microscopy elements

A

hyaline, mixed cellular cast, MT, Trichomonas

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9
Q

Interference-Contrast Microscopy Types

A

A. Nomarski (Differential)
B. Hoffmann (Modulation)

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10
Q

Bright-Field microscopes can be adapted

A

Interference-Contrast Microscopy

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11
Q

3-D microscopy image and layer-by-layer imaging of a specimen

A

Interference-Contrast Microscopy

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12
Q

SternheimerMalbin components

A

(CV + Safranin O)

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13
Q

Enhances nuclear detail

A

0.5% Toluidine Blue

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14
Q

% Acetic acid

A

2%

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15
Q

Hansel Stain components

A

Eosin Y + Methylene blue

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16
Q

Stains TAG and neutral fats (orangered)

A

Lipid stains

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17
Q

Lipid stains components

A

ORO and Sudan III

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18
Q

Urinary eosinophils

A

Hansel Stain

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19
Q

Crystals (normal) Moderate

A

5-20

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20
Q

Bacteria

A

Rare

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21
Q

Bacteria few

A

10-50

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22
Q

Casts Quantitated

A

Per LPF

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23
Q

RBCs

A

Per HPF

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24
Q

WBCs Quantitated

A

Per HPF

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25
SEC Frequency
Rare, Few, Moderate, Many
26
TEC, yeasts Frequency
Rare, Few, Moderate, Many
27
RTE cells Frequency
Average
28
Abnormal crystals and casts Frequency
Average
29
Red blood cells Normal value range: _________/HPF
30
Red blood cells Hypertonic urine = __________________________
CRENATED
31
Red blood cells Hypotonic urine = __________________________
32
Glomerular bleeding/damage= __________________________
RBC CASTS
33
White blood cells Normal value range: _______
0-5 /HPF
34
granules undergo Brownian Movement
GLITTER CELLS
35
Neutrophils *clinically insignificant
Glitter Cells
36
Eosinophils o Normal value range: __________
<1% Toluidine Blue
37
White blood cells Stain
Sternheimer-Malbin Stain
38
Glitter cells stain color
(Pale blue)
39
Leukocytes stain color
(Pale pink)
40
Eosinophils Clinically significant: __________ (associated with drug-induced interstitial nephritis)
41
Mononuclear cells (___________________)
LYMPHO, MONO, MACS, HISTIOCYTES
42
Largest cell
Squamous epithelial cell
43
Squamous epithelial cell size
30-40 um
44
_______________: SEC studded with Gardnerella vaginalis; associated with bacterial vaginosis
CLUE CELLS
45
Transitional epithelial cell (____________)
UROTHELIAL
46
Spherical, polyhedral or caudate with centrally located nucleus
Transitional epithelial cell
47
o Most clinically significant epithelial cell
Renal tubular epithelial (RTE) cell
48
Oblong or round to oval or rectangular and contain an eccentric nucleu
Renal tubular epithelial (RTE) cell
49
Renal tubular epithelial (RTE) cell >2 RTE/HPF indicates ___________________
TUBULAR DAMAGE
50
Renal tubular epithelial (RTE) cell Variations i. _______________ ii. _______________
OVAL FAT BODIES BUBBLE CELLS
51
Seen in lipiduria (i.e. nephrotic syndrome)
OVAL FAT BODIES (Lipid-containing RTE cell)
52
Lipid-containing RTE cell Identification
 Lipid Stains  Polarizing Microscope
53
 Lipid Stains
TAG and Neutral fats
54
 Polarizing Microscope (MALTESE-CROSS)
OVAL FAT BODIES
55
o UTI: ___________________ MOST FREQUENTLY ASSOCIATED
Bacteria
56
Most common cause of UTI: _________________________
ENTEROBACTERIACEAE (GRAM NEG)
57
Most frequent parasite encountered in urine urine
Trichomonas vaginalis
58
Pear-shaped flagellate (RAPID DARTING motility)
Trichomonas vaginalis
59
Trichomonas vaginalis Method of reporting: ___________
RARE, FEW, MODERATE, MANY PER HPF
60
associated with bladder cancer
Schistosoma haematobium ova
61
Specimen: 24hr unpreserved urine
Schistosoma haematobium ova
62
 Most common fecal contaminant
Enterobius vermicularis
63
True yeast infection: ____________________
WITH WBC
64
= seen in DM patients and IMMUNOCOMPROMISED
Candida albicans
65
Mucus thread o Major constituent: ________________
TAMM- HORSEFALL PROTEIN (UROMODULIN)
66
Factors that favors cast formation Low pH: ______________________
1. Urine stasis 2. High salt concentration 3. Low pH
67
CASTS (CYLINDRURIA) Major constituent: _______________ produced by _______
UROMODULIN RTE CELLS
68
Beginning of all casts
Hyaline cast
69
Hyaline cast Normal Value: ___________
0-2 PER LPF
70
Physiologic: Emotional stress, exercise, dehydration, heat exposure Pathologic: Glomerulonephritis, pyelonephritis, CHF
Hyaline cast
71
Nephron bleeding
RBC cast
72
Glomerulonephritis, proteinuria, dysmorphic RBCs # PER LPF
RBC cast
73
Inflammation within the nephron
WBC cast
74
Granules are derived from the lysosomes of RTE cells during normal metabolism
Granular casts
75
Granular casts Glomerulonephritis, Pyelonephritis, Stress, _______________
76
Final degenerative form of all types of casts
Waxy cast
77
Chronic renal failure
Waxy cast
78
Broad cast Aka ____________________
79
Factors affecting precipitation _________________ _________________ _________________
80
URINARY CRYSTALS usually reported as ________/____
RARE, FEW, MODERATE, MANY PER LPF
81
URINARY CRYSTALS abnormal crystals may be averaged ___
AVERAGE or HIGH
82
Brick dust / yellowbrown granules
Amorphous urates
83
frequently encountered as pink sediment (uroerythrin)
Amorphous urates
84
Heat and Alkali fluid
Amorphous urates
85
Ethylene glycol poisoning
CaOx
86
mistaken as RBC; suggests possibility of a calculus
CaOx
87
“cigarettebutt”
Calcium sulfate
88
Heat with HAc
Ammonium biurate
89
“magnesium phosphate crystals”
Calcium phosphate/apatite
90
“Magnesium ammonium phosphate” “coffin lid” “fern leaf”
Triple phosphate
91
Gas from HAc
Calcium carbonate
92
Mistaken as Uric acid crystals
Cystine
93
Cystinuria Cystinosis
Cystine
94
birefringence
Cystine
95
Rectangular plates w/ notched edges
Cholesterol crystal
96
(staircase pattern)
Cholesterol crystal
97
Nephrotic syndrome (lipiduria)
Cholesterol crystal
98
seen in conjuction with fatty casts and OFB
Cholesterol crystal
99
*heavy proteinuria
Cholesterol crystal
100
Chloroform
Cholesterol crystal
101
“sheaves of wheat”
Sulfonamide
102
“petals”
Sulfonamide
103
Mistaken as calcium phosphates
Sulfonamide
104
Soluble in HAc while positive with Lignin and Diazo rxn
Sulfonamide
105
Lignin Test (Old Newspaper test) = urine + 25% HCl=
yellow color (+)
106
Colorless needles that form bundles after refrigeration
Ampicillin
107
precipitates with tyrosine after the addition of alcohol
Leucine
108
Liver disorder
Leucine
109
Colorless to yellow needles in clumps or rosette
Tyrosine
110
Liver disease (more common that leucine)
Tyrosine
111
Clumped needles or granules with HAc, HCl, bright yellow color (+ in bilirubin rgt strip)
Bilirubin
112
Liver disease (most common)
Bilirubin
113
NaOH, ether, chloroform
Bilirubin
114
[?] mL of urine
10 to 15
115
Centrifuge at [?]
400 RCF for 5 mins
116
Decant urine ((?) remains)
0.5-1 mL
117
Transfer [?] to glass slide with 22 x 22 mm coverslip
20 uL
118
Examine (?)
10 LPF and 10 HPF
119
Quantitative measure of formed elements using a hemocytometer
Addis Count
120
Addis Count  Specimen
12 hour urine
121
Addis Count  Preservative
Sodium Flouride
122
Normal Values RBCs WBCs & ECs Hyaline Casts
0 - 500,000 0 - 1,800,00 0 - 5,000
123
Used for routine urinalysis
Bright-Field Microscopy
124
Identification of cholesterol molecules in OFB, fatty casts and crystals
Polarizing Microscopy
125
Identification of Treponema spp
Dark-Field Microscopy
126
For fluorescent microorganisms or those stained with a fluorescent dye
Fluorescence Microscopy
127
Delineates structure and contrasting colors of the nucleus and cytoplasm
SternheimerMalbin (CV + Safranin O)
128
Enhances nuclear detail
0.5% Toluidine Blue
129
Lyses RBCs and enhances nucleus of WBCs
2% Acetic acid
130
Differentiates Gram (-) to Gram (+) bacteria
GS
131
Stains eosinophilic granules
Hansel Stain
132
Lipid stains components
ORO and Sudan III
133
Distinguishes RBCs from WBCs, yeasts, oil droplets and crystals
2% Acetic acid
134
WBCs, ECs, and casts
SternheimerMalbin
135
Differentiates WBCs and RTE cells
0.5% Toluidine Blue
136
Identifies free fat droplets and lipidcontaining cells & casts
Lipid stains
137
Bacterial casts
GS
138
Identifies hemosiderin granules in casts and cells
Prussian Blue
139
Quantitated per LPF
Epithelial Cell Mucus Thread SEC Per LPF
140
Quantitated Per HPF
RBCs WBCs Crystals (normal) Bacteria TEC, yeasts OFB Abnormal crystals and casts
141
Quantitated Per 10 HPF
RTE cells
142
None: 0
143
Rare: 0-5
Epithelial Cell
144
Rare: 0-2
Crystals (normal)
145
Rare: 0-10
Bacteria
146
Rare: 0-1
Mucus Thread
147
Few: 5-20
Epithelial Cell
148
Few: 2-5
Crystals (normal)
149
Few: 10-50
Bacteria
150
Few: 1-3
Mucus Thread
151
Moderate: 20-100
Epithelial Cell
152
Moderate: 5-20
Crystals (normal)
153
Moderate: 50-200
Bacteria
154
Moderate: 3-10
Mucus Thread
155
Many: >100
Epithelial Cell
156
Many: >20
Crystals (normal)
157
Many: >200
Bacteria
158
Many: >10
Mucus Thread
159
Numerical ranges: 0-2, 2-5, 5-10, >10
Casts
160
Numerical ranges: 0-2, 2-5, 5-10, 10-25, 25-50, 50-100, >100
RBCs WBCs
161
Average
OFB
162
*quantitate an average of [?].
10 representative fields
163
Do not quantitate [?], but note their presence.
budding yeast, mycelia, elements, Trichomonas, or sperm
164
Urinary Sediment Constituents : A. CELLS
1. Red blood cells (Hematuria) 2. White blood cells (Pyuria) 3. Epithelial Cells 4. Bacteria 5. Parasites 6. Yeasts 7. Spermatozoa 8. Mucus thread
165
Urinary Sediment Constituents : CASTS
Hyaline cast RBC cast WBC cast RTE cell cast Bacterial cast Granular casts Fatty cast Waxy cast Broad cast Pigmented cast Mixed cellular cast Crystal cast
166
Urinary Sediment Constituents : CRYSTALS - ACID
Amorphous urates CaOx Calcium sulfate Hippuric acid Acid urate Sodium urate
167
Urinary Sediment Constituents : NORMAL CRYSTALS - Alkaline
Amorphous phosphate Ammonium biurate Calcium phosphate/apatite Dicalcium phosphate Triple phosphate Calcium carbonate
168
Urinary Sediment Constituents : ABNORMAL CRYSTALS - Acid
Cystine Cholesterol crystal Sulfonamide Ampicillin Leucine Tyrosine Bilirubin
169
Urinary Sediment Constituents : ABNORMAL CRYSTALS - Acid/Neutral
Sulfonamide Ampicillin Leucine Tyrosine
170
Smooth, non-nucleated, biconcave disks-shaped sediment (7um in diameter)
Red blood cells
171
Sources of error: Yeast, oil droplets, air bubbles, CaOx monohydrate
Red blood cells
172
Remedy: Addition of 2% HAc to lyse RBCs
Red blood cells
173
Clinical Significance: Glomerular membrane damage, vascular injury within GUT, glomerular bleeding, glomerulonephritis, renal calculi, malignancies, Schistosomiasis, strenuous exercise
Red blood cells
174
predominant
Neutrophils
175
Granulated and multilobed
Neutrophils
176
Swells in hypotonic (dilute and alkaline) urine and granules undergo Brownian Movement (Glitter Cells) *clinically insignificant
Neutrophils
177
Shrinks in hypertonic (acidic) urine
Neutrophils
178
Present in small amount
Mononuclear cells
179
An increase indicates an inflammatory response or renal transplant rejection
Mononuclear cells
180
Clinical Significance: infection or inflammation in the GUT
Mononuclear cells
181
Bacterial infection: cystitis, pyelonephritis, prostatitis, urethritis
Mononuclear cells
182
Non-bacterial infection: glomerulonephritis, SLE, tumors
Mononuclear cells
183
Largest cell (30-40 um) with abundant, irregular cytoplasm and prominent nucleus
Squamous epithelial cell
184
Folded cell may resemble casts. Found in the linings of vagina, female urethra and lower portion of male urethra
Squamous epithelial cell
185
o Spherical, polyhedral or caudate with centrally located nucleus
Transitional epithelial cell
186
o From linings of renal pelvis, calyces, ureter, urinary bladder and upper portion of male urethra.
Transitional epithelial cell
187
o Significant numbers can be seen after catheterization (single, pairs, or in clumps {syncytia})
Transitional epithelial cell
188
Oblong or round to oval or rectangular and contain an eccentric nucleus and coarsely granulated cytoplasm
Renal tubular epithelial (RTE) cell
189
o Lipid-containing RTE cell
190
o Seen in lipiduria (i.e. nephrotic syndrome)
191
Identification of RTE
 Lipid Stains  Polarizing Microscope
192
o RTE cell with nonlipid-filled vacuoles
193
o Seen in acute tubular necrosis
194
may be mistaken for WBCs
RTEs from the distal convoluted tubules
195
Reporting of Epithelial Cells RFMoMa
o Squamous (LPF) o Transitional (HPF)
196
Reporting of Epithelial Cells Ave. number per HPF
o RTE Cell o Oval Fat Bodies
197
Parasites
Trichomonas vaginalis Schistosoma haematobium ova Enterobius vermicularis
198
Trichomonas vaginalis
Pear-shaped flagellate
199
 Blood fluke with terminal spine
Schistosoma haematobium ova
200
 Causes hematuria
Schistosoma haematobium ova
201
Small, refractile oval structures that may or may not bud
Yeasts
202
seen in DM patients and _____________
Candida albicans
203
 formed in the DCT and collecting ducts
CASTS (Cylindruria)
204
Factors that favors cast formation
1. Urine stasis 2. High salt concentration 3. Low pH
205
Formation of Casts
1. Aggregation and concentration of THP into fibrils that attaches to RTE cells. 2. Interweaving of protein fibrils into a mesh-like pattern. 3. (Possible) attachment of urinary sediments to the matrix 4. Detachment and excretion
206
Order of Cast Degeneration
207
Pathologic: glomerulonephritis, pyelonephritis, CHF
Hyaline cast
208
May be within the nephron confused with EC casts *use phase contrast or a supravital stain
WBC cast
209
Pyelonephritis Acute interstitial nephritis
WBC cast
210
Advanced tubular destruction Renal tubular damage
RTE cell cast
211
Pyelonephritis
6Bacterial cast cast
212
Identification using Gram Staining
Bacterial cast cast
213
Not stained by SternheimerMalbin stain
Fatty cast
214
Identified using lipid stains and polarizing microscope
Fatty cast
215
Neph rotic syndrome (Lipiduria)
Fatty cast
216
Brittle, highly refractile with jagged edges
Waxy cast
217
Stasis of urine flow
Waxy cast
218
Indicates widening of the tubular walls
Broad cast
219
Extrem e urine stasis
Broad cast
220
Renal failure
Broad cast
221
Hyaline matrix with coloration due to pigmentation incorporation
Pigmented cast
222
Incorporated bilirubin
(golden brown)
223
Hemoglobin or myoglobin
(yellow to red brown)
224
Casts containing multiple cell types
Mixed cellular cast
225
Glomerulonephritis (RBC and WBCs)
Mixed cellular cast
226
Pyelonephritis (WBC, RTE casts or WBC and bacterial casts)
Mixed cellular cast
227
Casts containing urates, CaOx, and sulfonamides are occasionally seen
Crystal cast
228
Deposition of crystals in the tubule or collecting duct
Crystal cast
229
formed by precipitation of urinary solutes (organic compounds, salts, medication, etc.)
URINARY CRYSTALS
230
“2 pyramids joined at their bases”
Dihydrate
231
oval or Dilute HCl; Insoluble in HAc dumbbell Calcium sulfateshaped
Monohydrate
232
Yellowbrown/colorless elongated prisms
Hippuric acid
233
large granules and may have spicules
Acid urate
234
Slender prisms arranged in fan or sheaf like structures
Sodium urate
235
Granular; white ppt
Amorphous phosphate
236
Dilute HAc
Amorphous phosphate Calcium phosphate/apatite Triple phosphate
237
Gas from HAc
Calcium carbonate
238
insoluble by heat
Calcium phosphate/apatite
239
Heat with HAc
Ammonium biurate
240
Water and Ether
Hippuric acid
241
Acetic aci d
Calcium sulfate
242
Insoluble in HAc
CaOx
243
- basic calcium PO4
a. Hydroxyapatite
244
- calcium hydrogen PO4
b. Brushite
245
Long slender prisms with one end pointed; found in clusters
Dicalcium phosphate
246
Presence of ureasplitting bacteria
Ammonium biurate Triple phosphate
247
Small, colorless, dumbbell-shaped.
Calcium carbonate
248
Formation of gas (effervescence) after the addition of acetic acid
Calcium carbonate
249
Colorless, hexagonal plates (Piattos)
Cystine
250
Dilute HCl and Ammonia
Cystine
251
colorless to yellow needles
Tyrosine
252
soluble in HAc, while sulfonamide is positive with Lignin and Diazo rxn
Sulfonamide
253
Solubility: Acetone
Sulfonamide
254
Solubility: Refrigeration = bundles
Ampicillin
255
1. volume of sediment after decantation
256
2. volume of sediment for microscopic examination
257
3. produces a 3-D image by differential contrast
258
4. two components of Hansel stain
259
5. most significant epithelial cell
260
6. reporting of #5
261
7. quantity of #5 considered as significant
262
8. condition indicated by #7
263
9. RBCs in hypotonic are known as _____
264
10. WBCs with sparkling appearance
265
11. What type of WBC is #10?
266
12. most frequently seen parasite in urine
267
13. When does the presence of spermatozoa in urine reported?
268
14. more common form of calcium oxalate
269
15. color of amorphous urates in fresh urine
270
16. compounds that precipitate in acidic urine forming crystals
271
17. stain used for the identification of eosinophils
272
18. stains used to confirm the presence of oval fat bodies
273
19. parasite considered as most common fecal contaminant
274
20. primary factor affecting in vitro crystallization
275
21. metabolic disorder associated with uric acid crystals
276
22. percentage of mononuclear cells implicated in chronic inflammation
277
23. bubble cell is originally what specific cell
278
24. primary factor affecting in vivo crystallization
279
25. sediment that may accompany S. haematobium
280
POINTS to PONDER..... A 2-year-old left unattended in the garage for 5 minutes is suspected of ingesting antifreeze. What type of crystal would you expect to be present? What urine pH correlates with this crystal?
281
of urinary sediment is the third part of routine urinalysis, after physical and chemical examination.
Microscopic examination
282
The purpose is to detect and to identify [?] present in the urine.
insoluble materials
283
Since some urinary sediments are of no clinical significance and others are considered normal unless they are present in increased amounts, examination of the urinary sediment must include both [?] of the elements present.
identification and quantitation
284
Take note of the following under the LPO:
285
Shift to HPO and take note of the presence of the following:
286
Microscopic Quantitation: Epithelial cells/LPF Crystals/HPF Bacteria/HPF RBCs/HPF WBCs/HPF Casts/LPF Mucous threads
287
Do not quantitate [?], but do note their presence based on laboratory practice.
budding yeasts, mycelial elements, trichomonas or sperms
288
Heat and alkali flui
Amorphous urates
289
Dilute HCl; Insoluble in HAc
CaOx
290
Acetic acid
Calcium sulfate
291
Water and ether
Hippuric acid
292
Dilute HAc
Amorphous phosphate Triple phosphate
293
Heat with HAc
Ammonium biurate
294
Dilute HAc; Insoluble by heat
Calcium phosphate/apatite
295
Gas from HAc
Calcium carbonate
296
Seen in old specimens
Ammonium biurate
297
Colorless, hexagonal plates (piattos)
Cystine
298
Cyanide-nitropruside test Sulivan test
Cystine
299
Colorless to yellow-brown needles
Sulfonamide
300
Overdose of penicillin drugs
Ampicillin
301
Oily-looking spheres with concentric circles and radial striations
Leucine