[1] LESSON 6: URINALYSIS—MICROSCOPIC EXAMINATION OF URINE Flashcards
May indicate the presence of certain sediments
Color
Pathologic or non-pathologic causes of turbidity
Clarity
Blood
RBCs, RBC Cast
Protein
Casts, cells
Nitrite
Bacteria, WBCs
LE
WBCs, WBC Casts, bacteria
Visualization of elements with low refractive indices
Phase-Contrast Microscopy
Phase-Contrast Microscopy elements
hyaline, mixed cellular cast, MT, Trichomonas
Interference-Contrast Microscopy Types
A. Nomarski (Differential)
B. Hoffmann (Modulation)
Bright-Field microscopes can be adapted
Interference-Contrast Microscopy
3-D microscopy image and layer-by-layer imaging of a specimen
Interference-Contrast Microscopy
SternheimerMalbin components
(CV + Safranin O)
Enhances nuclear detail
0.5% Toluidine Blue
% Acetic acid
2%
Hansel Stain components
Eosin Y + Methylene blue
Stains TAG and neutral fats (orangered)
Lipid stains
Lipid stains components
ORO and Sudan III
Urinary eosinophils
Hansel Stain
Crystals (normal) Moderate
5-20
Bacteria
Rare
Bacteria few
10-50
Casts Quantitated
Per LPF
RBCs
Per HPF
WBCs Quantitated
Per HPF
SEC Frequency
Rare, Few, Moderate, Many
TEC, yeasts Frequency
Rare, Few, Moderate, Many
RTE cells Frequency
Average
Abnormal crystals and casts Frequency
Average
Red blood cells
Normal value range: _________/HPF
Red blood cells
Hypertonic urine = __________________________
CRENATED
Red blood cells
Hypotonic urine = __________________________
Glomerular bleeding/damage= __________________________
RBC CASTS
White blood cells
Normal value range: _______
0-5 /HPF
granules undergo Brownian Movement
GLITTER CELLS
Neutrophils
*clinically insignificant
Glitter Cells
Eosinophils
o Normal value range: __________
<1% Toluidine Blue
White blood cells Stain
Sternheimer-Malbin Stain
Glitter cells stain color
(Pale blue)
Leukocytes stain color
(Pale pink)
Eosinophils
Clinically significant: __________ (associated with drug-induced interstitial nephritis)
Mononuclear cells (___________________)
LYMPHO, MONO, MACS, HISTIOCYTES
Largest cell
Squamous epithelial cell
Squamous epithelial cell size
30-40 um
_______________: SEC studded with Gardnerella vaginalis; associated with bacterial vaginosis
CLUE CELLS
Transitional epithelial cell (____________)
UROTHELIAL
Spherical, polyhedral or caudate with centrally located nucleus
Transitional epithelial cell
o Most clinically significant epithelial cell
Renal tubular epithelial (RTE) cell
Oblong or round to oval or rectangular and contain an eccentric nucleu
Renal tubular epithelial (RTE) cell
Renal tubular epithelial (RTE) cell
> 2 RTE/HPF indicates ___________________
TUBULAR DAMAGE
Renal tubular epithelial (RTE) cell
Variations
i. _______________
ii. _______________
OVAL FAT BODIES
BUBBLE CELLS
Seen in lipiduria (i.e. nephrotic syndrome)
OVAL FAT BODIES (Lipid-containing RTE cell)
Lipid-containing RTE cell Identification
Lipid Stains
Polarizing Microscope
Lipid Stains
TAG and Neutral fats
Polarizing Microscope (MALTESE-CROSS)
OVAL FAT BODIES
o UTI: ___________________ MOST FREQUENTLY ASSOCIATED
Bacteria
Most common cause of UTI: _________________________
ENTEROBACTERIACEAE (GRAM NEG)
Most frequent parasite encountered in urine urine
Trichomonas vaginalis
Pear-shaped flagellate (RAPID DARTING motility)
Trichomonas vaginalis
Trichomonas vaginalis
Method of reporting: ___________
RARE, FEW, MODERATE, MANY PER HPF
associated with bladder cancer
Schistosoma haematobium ova
Specimen: 24hr unpreserved urine
Schistosoma haematobium ova
Most common fecal contaminant
Enterobius vermicularis
True yeast infection: ____________________
WITH WBC
= seen in DM patients and IMMUNOCOMPROMISED
Candida albicans
Mucus thread
o Major constituent: ________________
TAMM- HORSEFALL PROTEIN (UROMODULIN)
Factors that favors cast formation
Low pH: ______________________
- Urine stasis
- High salt concentration
- Low pH
CASTS (CYLINDRURIA)
Major constituent: _______________ produced by _______
UROMODULIN
RTE CELLS
Beginning of all casts
Hyaline cast
Hyaline cast
Normal Value: ___________
0-2 PER LPF
Physiologic: Emotional stress, exercise, dehydration, heat exposure
Pathologic: Glomerulonephritis, pyelonephritis, CHF
Hyaline cast
Nephron bleeding
RBC cast
PER LPF
Glomerulonephritis, proteinuria, dysmorphic RBCs
RBC cast
Inflammation within the nephron
WBC cast
Granules are derived from the lysosomes of RTE cells during normal metabolism
Granular casts
Granular casts
Glomerulonephritis, Pyelonephritis, Stress, _______________
Final degenerative form of all types of casts
Waxy cast
Chronic renal failure
Waxy cast
Broad cast
Aka ____________________
Factors affecting precipitation _________________ _________________ _________________
URINARY CRYSTALS
usually reported as ________/____
RARE, FEW, MODERATE, MANY PER LPF
URINARY CRYSTALS
abnormal crystals may be averaged ___
AVERAGE or HIGH
Brick dust / yellowbrown granules
Amorphous urates
frequently encountered as pink sediment (uroerythrin)
Amorphous urates
Heat and Alkali fluid
Amorphous urates
Ethylene glycol poisoning
CaOx
mistaken as RBC; suggests possibility of a calculus
CaOx
“cigarettebutt”
Calcium sulfate
Heat with HAc
Ammonium biurate
“magnesium phosphate crystals”
Calcium phosphate/apatite
“Magnesium ammonium phosphate” “coffin lid” “fern leaf”
Triple phosphate
Gas from HAc
Calcium carbonate
Mistaken as Uric acid crystals
Cystine
Cystinuria Cystinosis
Cystine
birefringence
Cystine
Rectangular plates w/ notched edges
Cholesterol crystal
(staircase pattern)
Cholesterol crystal
Nephrotic syndrome (lipiduria)
Cholesterol crystal
seen in conjuction with fatty casts and OFB
Cholesterol crystal
*heavy proteinuria
Cholesterol crystal
Chloroform
Cholesterol crystal
“sheaves of wheat”
Sulfonamide
“petals”
Sulfonamide
Mistaken as calcium phosphates
Sulfonamide
Soluble in HAc while positive with Lignin and Diazo rxn
Sulfonamide
Lignin Test (Old Newspaper test) = urine + 25% HCl=
yellow color (+)
Colorless needles that form bundles after refrigeration
Ampicillin
precipitates with tyrosine after the addition of alcohol
Leucine
Liver disorder
Leucine
Colorless to yellow needles in clumps or rosette
Tyrosine
Liver disease (more common that leucine)
Tyrosine
Clumped needles or granules with HAc, HCl, bright yellow color (+ in bilirubin rgt strip)
Bilirubin
Liver disease (most common)
Bilirubin
NaOH, ether, chloroform
Bilirubin
[?] mL of urine
10 to 15
Centrifuge at [?]
400 RCF for 5 mins
Decant urine ((?) remains)
0.5-1 mL
Transfer [?] to glass slide with 22 x 22 mm coverslip
20 uL
Examine (?)
10 LPF and 10 HPF
Quantitative measure of formed elements using a hemocytometer
Addis Count
Addis Count
Specimen
12 hour urine