1. Introduction to Bacteriology Flashcards

1
Q

Prokaryote

A

Prokaryotes are organisms whose cells lack a nucleus and other organelles. Prokaryotes are divided into two distinct groups: the bacteria and the archaea.

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2
Q

Eukaryote

A

Eukaryotes are organisms whose cells contain a nucleus and other membrane-bound organelles. There is a wide range of eukaryotic organisms, including all animals, plants, fungi, and protists, as well as most algae.

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3
Q

Bacterial components

A
  1. Glycocalyx
  2. Flagella
  3. Pili/Fimbriae
  4. Cell wall
  5. Cell Membrane
  6. Cytoplasm
  7. Nucleoid
  8. Plasmid
  9. Ribosomes
  10. Inclusions
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4
Q

Glycocalyx

A
  • Substance that surround cells
  • If organized and firmly attached to the cell wall = capsule
  • If unorganized and loosely attached to the cell wall = slime layer
  • Important component of biofilm (helps cell to attach to various
    surfaces and to each other)
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5
Q

Flagella

A

Long filamentous appendages for movement of bacteria

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6
Q

Types of flagella

A
  1. Peritrichous (distributed over entire cell)
    e.g. Escherichia coli
  2. Monotrichous and polar (a single flagellum at one poles or
    ends of the cell)
    e.g. Vibrio cholerae
  3. Lophotrichous and polar (a tuft of flagella coming from one
    pole)
    e.g. Helicobacter pylori
  4. Amphitrichous and polar (at both pole of the cell)
    e.g. Campylobacter jejuni
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7
Q

Pili / Fimbriae

A
  • Hairlike appendages – shorter, straighter, thinner than flagella
  • Fimbriae:
    #occur at the poles or evenly distributed over the entire surface of the cell
    #a few to several hundred per cell
  • Pili:
    #Longer than fimbriae
    #One or two per cell
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8
Q

Cell wall

A
  • Complex and semi-rigid structure

- Determine the shape of bacteria

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9
Q

Cell (cytoplasmic) membrane

A
  • Contains phospholipids

- Enclosing the cytoplasm

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10
Q

Cytoplasm

A
  • Contain 80% water and primarily protein, carbohydrates, lipids, inorganic ions and low-molecular-weight compounds
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11
Q

Nucleoid

A
  • Chromosomal DNA is located
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12
Q

Plasmid

A
  • Small, circular, supercoiled, double-stranded DNA
  • Extrachromosomal genetic elements, not connected to bacterial
    chromosome
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13
Q

Ribosomes

A

-A ribosome is an intercellular structure made of both RNA and protein, and it is the site of protein synthesis in the cell

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13
Q

Ribosomes

A

-A ribosome is an intercellular structure made of both RNA and protein, and it is the site of protein synthesis in the cell

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14
Q

Inclusions

A
  • found in bacteria as particles of aggregated protein
  • represent sites of viral multiplication in a bacterium or a
    eukaryotic cell and usually consist of viral capsid proteins.
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15
Q

The formation of endospore

A
  1. DNA is replicated
  2. Cellular division of cytoplasmic membrane
  3. Prespore formation begins
  4. Cortex formation
  5. Spore coat formation begins
  6. Maturation begins Exosporium formation
  7. Mother spore releases mature spore (endospore)
16
Q

Shape of bacterial

A
  1. Coccus
  2. Bacillus/ Rod
  3. Spiral
17
Q

Arrangement of cocci bacteria

A
  1. Diplococci
  2. Streptococci
  3. Tetrad
  4. Sarcinae
  5. Staphylococci
18
Q

Arrangement of Bacillus/ Rod Bacteria

A
  1. Single Bacilli
  2. Diplobacilli
  3. Streptobacilli
  4. Coccobacilli
19
Q

Arrangement of Spiral Bacteria

A
  1. Vibrio (curved rod)
  2. Spirilum (helical)
  3. Spirochete (helical and flexible)
20
Q

Gram-Positive Cell Wall

A
  • thick layers of peptidoglycan = form thick & rigid structure
  • Prominent component = teichoic acids
  • X outer membrane, lipopolysaccharide, porin proteins, periplasm
21
Q

Functions of teichoic acid

A
  1. Bind and regulate movement of cations
  2. Prevent extensive wall breakdown and cell lysis
  3. Provide antigenic specificity (to identify gram +ve bacteria)
22
Q

Gram-Negative cell wall

A
  • thin layers of peptidoglycan & an outer membrane
  • X teichoic acids
  • has outer membrane, lipopolysaccharide, porin proteins & periplasm
23
Q

Types of staining

A
  1. Simple staining
  2. Differential staining
  3. Special staining
24
Q

Simple staining

A
  • only one dye is used

- differentiation among bacteria is impossible

25
Q

Differential staining

A
  • more than one dye is used

- differentiation among bacteria is possible

26
Q

Special staining

A
  • more than one dye is used

- special structure are seen

27
Q

Gram staining

A
  1. Crystal violet
  2. Lugol’s iodine
  3. Acetone-iodine
  4. Safranin
28
Q

Acid-fast stain basic requirements

A
  1. Primary and mordant staining with strong Carbol Fuchsin
  2. Decolourization with Acid Alcohol
  3. Counterstain with Methylene Blue
29
Q

Classification of Bacteria

A
  1. Shape and arrangement
  2. Ram stain reaction
  3. Biochemical and growth characteristics
  4. Antigenic structure