03 - Amoebae, Trichomonads, Histomonads Flashcards
(Pathogenic Free-Living Amoebae)
- name two species
- normally inhabit what?
- anaerobic, aerobic, or facultative?
- naegleria spp. & acanthamoeba spp.
- soil, stagnant water, sewage
- facultative (parasites of humans and animals)
(Pathogenic Free-Living Amoebae)
(Acanthamoeba spp.)
- most common amoeba of freshwater and soil
- can enter what if immunocompromised?
- most commonly enters via what?
- CNS
- broken skin
(contact lenses and corneal abrasians –> keratitis)
(Pathogenic Free-Living Amoebae)
(Naegleria fowleri)
- where do they live?
- when does it proliferate?
- found in what?
- stagnant bodies of water, thermal pools (resistant to heat), unhygenic swimming pools
- hot, dry, conditions
- humans, dogs, sheep
- What causes primary amoebic meningoencephalitis?
- naegleria fowleri
(then read)

(The flagellates)
- Sub-kingdom?
- phylum?
- sub-phylum?
- protozoa
- sarcomastigophora
- mastigophora
(The flagellates)
1-2 What are the two major divisions?
- mucosoflagellates (trichomonads, histomonads, giardia)
- hemoflagellates (trypanosomes, leishmanias)
(Clicker)
- amoebae are important parasites in humans as well as dogs - true or false?
- true
(Clicker)
- Naegleria typically infect humans via what?
- the nose

D
(The flagellates)
(Trichomonoads)
(say which animal affected)
- tritrichomonas foetus -
- trichomonas gallinae -
- trichomonas vaginalis -
- tetratrichomonas gallinarum -
- cattle, cats
- pigeons
- humans
- galliform bird
(flagellates)
(tritrichomonas foetus)
- host?
- where in female?
- in male?
- cattle
- uterus
- preputial cavity (sheath)
(flagellates)
(tritrichomonas foetus)

(flagellates)
(tritrichomonas foetus)
- How many anterior flagella?
- 3
(then just read)

(flagellates)
(tritrichomonas foetus)
(life cycle)
- multiply how?
- have cysts?
- how transmitted?
- temporary in bulls?
- Cows infected via vagina and maintain infection in uterus.. what are three possible results?
- binary fission
- no
- coitus, AI
- bulls stay persistently infected
- some self cure in 3 months, some develop sterile immunity, some become permanent carriers
(flagellates)
(tritrichomonas foetus)
(pathogenesis)
(bulls)
- infection maintained where?
- symptoms?
- does it affect fertility or breeding efficiency?
(cows)
- usually produces what?
- can it cause abortion?
- infection may lead to retained placenta
- if severe endometritis can cause what?
- preputial cavity
- usually symptomatic
- no
- low grade endometritis
- yes (early term)
- sterility
(flagellates)
(tritrichomonas foetus)
(Diagnosis)


(flagellates)
(tritrichomonas foetus)
(cats)


(flagellates)
(Trichomonas Gallinae)
- parasite of what in birds?
- hosts?
- produce ulceration and inflammation with extensive yellowish or greenish lesions
- lesions may extend to liver and lungs causing heavy mortality
- mouth, esophagus, and crop
- pigeons, morning doves, raptors that feed on pigeons, seen in chicken and turkeys




E

D

D

C
(clicker)
- Histomonas meleagridis liver lesions may be pathogonimc - true or false
- true
(Tetratrichomonas Gallinarum)
- affect what?
- where in body?
- transmission?
- Characteristic lesion?
- galliform birds (chicken and turkeys)
- lower intestine
- fecal/oral
- granulomatous, liver lesions
(Flagellates)
(Trichomonas Vaginalis)
- affect what?
- how transmitted?
- where in body?
- symptoms in male? in female?
- humans
- sexually transmitted
- vagina, urethra, prostate
- asymptomatic; vaginitis (w/discharge)
(?)

(the flagellates)
(Histomonas Meleagridis)
- Causative agent of what?
- Important disease of what two animals?
- blackhead
- chicken and turkey (esp. turkey!)
(the flagellates)
(Histomonas Meleagridis)
(Hosts)
- What are the definitive hosts?
- Which is severley affected?
- Which is often asymptomatic?
- intermediate host?
- transport host?
- gallinaceous birds
- Turkeys (also chukkar partride and ruff grouse… but just remember turkeys)
- Chickens (also peafowl, guinea hens, pheasants and bobwhite quail… just remember chickens)
- cecal worm (heterakis gallinarum)
- earthworm (can carry long time), flies, grasshoppers, sowbugs, crickets
(the flagellates)
(Histomonas Meleagridis)
- What is the distribution?
(structure)
- one form… or pleomorphic?
- where is flagellated form found?
- worldwide (cosmopolitan)
- pleomorphic (3 forms)
- lumen of the ceca
(the flagellates)
(Histomonas Meleagridis)
(Life Cycle)
- Reproduce How?
- principal transmission?
- binary fission
- ingesting eggs of heterakis gallinarum
(the flagellates)
(Histomonas Meleagridis)
(cecal worm - heterakis gallinarum)
- nematode of galliformes
- consumes flagellate form where?
- then what happens?
- flagellates then do what in cecal worms?

- in cecum
- flagellates multiply (therefore heterakis gallinarum are intermediate hosts…)
- penetrate oocytes –> infect eggs
(check out thing)

(Histomonas Meleagradis)
(Epidemiology)
- histomonads and cecal worms very common in what?
- What disease does this cause in turkeys?
what ages affected?
worst?
- chickens (wild birds can be asymptomatic carriers)
- black head disease
all
3-12 weeks (also affect young chickens)
(Histomonas Meleagridis)
(Pathogenesis)
- Invade wall of what? then do what?
- cause what in liver? when?
- cecum, produce lesions (8th day), travel to liver via blood
- lesions (pathognomonic)/liver enlarges; by 10th day
(Histomonas Meleagradis)
(clinical signs)
- what colored droppings?
- four more signs
- can it cause death?
- sulfur
- drowsiness, anorexia, cyanotic head, fever
- yes
(the flagellates)
(Histomonas Meleagridis)
(diagnosis)
- how do you diagnose?
- history, lesions, signs, microscopic identification
(the flagellates)
(Histomonas Meleagridis)
(Prevention/Control)
- restrict access to what?
- preventative chemotherapy using what?
- chickens/earthworms/heterakis eggs
- nitarsone in feed
Don’t worry about this pp83-97 - guess we will be coming back to this

(Balamuthia Mandrillaris)
- Free living amoeba
- causes what in humans?
- can cause what deadly neurological condition?
- amoebiasis
- granulomatous amoebic encephalitis (GAE)