01 - General

Question 1

(stains)

(gram stain)

1. used to do what?
2. G+ look how?
3. G+ look how?

Answer 1

1. classify bacteria
2. deep blue
3. red

Question 2

(Romanowsky type)

1. for use on air-dried specimens
2. what is a common type of this stain?
3. mainstay in cytopathology why?

Answer 2

2. Diff-quick
3. impart different colors to cytoplasm and nucleus

Question 3

(new Methylene blue)

1. good for detection of what?
2. how is this useful?

Answer 3

1. nucleated cells, all bacteria, fungi, and yeast
2. evaluate nucleated cell populations in heavy blood contamination (RBCs stain poorly)

Question 4

(Prussian blue)

1. stains what?
2. does it detect loosely or tightly bound iron?

Answer 4

1. ferric (Fe3+) iron
2. loosely (so not iron in hemoglobin)

Question 5

(immunocytochemical stains)

1. use antibodies against epitopes, can be very specific and powerful
2. can be used to identify what?
3. limited only by the Abs available -> many of which are conserved across species

Answer 5

2. cell line of origin of unknown cells