Zoonotics: Pasteurella Multocida (Gram Neg Rod/Bacilli Bacteria)

Question 1

Pasteurella Multocida

Morphology:

Answer 1

• small, gram-negative,*
• nonmotile, non–spore-forming*

Catalase (Cat) Positive

Oxidase Positive

• coccobacillus with bipolar staining features*
• The bacteria typically appear as single bacilli on Gram stain; however, pairs and short chains can also be seen*

Question 2

Pasteurella Multocida

Reservoir / Transmission

Answer 2

Domestic Dog/CATS

Scratch

Bites

Note:

part of the normal Flora of the mouth fo Dogs & Cats

Question 3

Cellulitis

Define:

Answer 3

• Cellulitis is a common, potentially serious bacterial skin infection. The affected skin appears swollen and red and is typically painful and warm to the touch.*
• Cellulitis usually affects the skin on the lower legs, but it can occur in the face, arms and other areas. It occurs when a crack or break in your skin allows bacteria to enter.*
• Left untreated, the infection can spread to your lymph nodes and bloodstream and rapidly become life-threatening. It isn’t usually spread from person to person.*

Question 4

Pasteurella Multocida

Reservoir / Transmission

Answer 4

P multocida (CATS) often exists as a commensal in the upper respiratory tracts of many livestock, poultry, and domestic pet species, especially cats and dogs.

Pasteurella Canis species** (dogs) are some of the **most prevalent commensal bacteria** **present in domestic and wild animals worldwide.

-infection in humans is often associated with an animal bite, scratch, or lick, but infection without epidemiologic evidence of animal contact may occur

Question 5

Pasteurella Multocida

Virulence Factor

Answer 5

• Polysaccharide capsule
• Endotoxin cell wall
• NO EXOTOXIN

Question 6

• Pasteurella Multocida*
• Disease ?*

Answer 6

Cellulitis

Incubation (less than 24 hours)

Question 7

Pasteurella Multocida

S / S

Answer 7

• Cellulitis*
• Incubation period (less than 24 hrs)*

Osteomyelitis- with CAT bites!!!

May lead to “Necrotizing Fasciitis” or “Osteomyelitis”

Lymphadenopathy** spreading systemically with Pt’s with (**Liver disease or COPD)

Question 8

Pasteurella Multocida

Laboratory-Growns ?

Answer 8

Grows on 5% Sheeps Blood Agar

Under microscopy: Demonstrates Bipolar Staining

• Biochemical Tests*
• Serological Tests*

Question 9

Pasteurella Multocida

TX:

Answer 9

Penicillin G

Use Beta Lactamase inhibitor TO PREVENT RESISTANCE (Amoxicillin - Clavulanci Acid: combo)

Oral antimicrobial