Zoonotics: Brucella (Gram Neg Bacilli Bacteria)

Question 1

(Zoonotic)

Name all Gram negative

Rod/Bacilli:

5:

Answer 1

1. Bartonella Henselae
2. Brucella
3. Francisella Tularnsis
4. Pastuerella Multocidida
5. Yersinia enterocolitica

Question 2

Brucella

name the Disease

Answer 2

Brucelossis

Question 3

Brucella

Morphology:

Answer 3

1. Gram Negative (Rod/Bacilli)

2. Facultative Intracellular

• Facultative Intracellular : can live inside/outside host cells*
• : can live and multiply inside Macrophages by preventing phagolycism and fusion*

Question 4

Brucella

Reservoir:

Name of Organism: (4 types)

Answer 4

Reservoir:

1. Cow- B. Brucella Abortus

2. Pig - B. Suis

3. Canines- B. canis

4. Goat/sheep/camel- B. Melitensis

Question 5

Brucella

Transmission:

Answer 5

1. Direct contact: skin
2. Indirect contact: Unpastuarized Milk/cheeze

Question 6

Brucella

Virulence factor:

Answer 6

1. Coagulase

2. Fibrinolysis

3. Type 3 secretion

4. Endotoxin

Type3 secretion system (often written Type III secretion system TTSS or T3SS, also called Injectisome) is a protein appendage found in several Gram-negative bacteria. In pathogenic bacteria, the needle-like structure is used as a sensory probe to detect the presence of eukaryotic organisms and secrete proteins that help the bacteria infect them. en

Question 7

Brucella

Clinical Manifistation

Answer 7

Weight loss (anorexia intitially)

fever / chills

non-specific symptoms

Malaise

Question 8

Brucella

Lab diagnosis:

Answer 8

• Serology*
• ELISA*
• Culture*
• Aglutination test*

Question 9

Brucella

Treatment/prevention

Answer 9

1. Rafampin
2. Streptomycin
3. Tetracycline

Question 10

Brucella

Prevention:

Answer 10

-Animal vaccination with attenuated strain B-abortus

- Avoidance of infected material

-NO Human VACCINE-

Question 11

Brucellosis

Complications:

Answer 11

• -Can travel through multiple organs throughout the reticulo-endothelial system*
• -then replicating intracellularly to point of causing Host cell Lysis- spreading Systemically*
• • Causing enlargement of all affected organs :*

Spleen / liver / Lymph nodes

Question 12

Brucellosis

Complication of Chronic Brucella infection

Name:

Answer 12

1. Osteomyelitis (an infection of bone)

Symptoms may include pain in a specific bone with overlying redness, fever, and weakness.

The long bones of the arms and legs are most commonly involved in children, while the feet, spine, and hips are most commonly involved in adults.

Question 13

Brucelossis:

TX:

Answer 13

• 1. Tetracycline*
• 2. Doxocycline*
• 3. Tetracycline (with) Rifampin (adjunctive therapy)*