Wk 3 Genetic Epigenetic Mechanisms

Answer 1

GTP = active
GDP = inactive

Question 2

What is tubulin?

Answer 2

A dimeric GTPase

Question 3

Analogy for GTP

Answer 3

A timer

Question 4

Dynamic instability

Answer 4

p 6

Question 5

Why are stabilizing and destabilizing drugs both detrimental to microtubules?

Answer 5

Microtubules must be able to grow AND shrink appropriately to build functional structures.

Question 6

Why does dynamic instability allow for growth?

Question 7

Understand this slide, don’t memorize

Answer 7

Question 8

What is topoisomerase 1?

Answer 8

Topo 1 nicks DNA, stores the ATP required to make the nick, covalently attaches to the DNA,
-as replication fork comes along, Topo 1 jumps out of the way
-if Topo 1 doesn’t jump out of the way fast enough -> ds break

Question 9

What is topoisomerase 2?

Answer 9

-can do everything Topo 1 can do and -
- pass DNA strands through one another
- to get apart, must make ds break
- can solve replication problems
- -essential role in mitosis