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Biochem > What Replication Proteins Do > Flashcards

What Replication Proteins Do Flashcards

1
Q

ORC

DnaA

A

Initiate replication binding to origin

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2
Q

Cdc45/Mcm 2-7/GINS Complex (CMG Complex)

DnaB

A

Helicase unwinds DNA

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3
Q

Cdc6/cdt1

DnaC

A

Loads helicase

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4
Q

RPA

SSB

A

Facilitates unwinding and protects ssDNA

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5
Q

Topoisomerase 2

Gyrase

A

Relieves positive supercoils ahead of fork

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6
Q

Primase

DnaG

A

Synthesizes RNA primers

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7
Q

FEN1 & Pol alpha

Pol 1

A

FEN1 removes RNA primers
Pol alpha synthesizes short DNA as part of RNA-DNA

(Pol 1 for Pro does both)

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8
Q

Pol beta and epsilon

DNA pol 3

A

Primary replication polymerases

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9
Q

PCNA

Beta subunit

A

Ring clamp holds polymerase on DNA

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10
Q

RFC

Y complex

A

Loader of the ring

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11
Q

DNA Ligase

DNA Ligase

A

Seals Okazaki fragments into a continuous strand

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Biochem (16 decks)

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