Exam 1 Random 2

Question 1

splicing forms what linkage?

Answer 1

2’-5’

Question 2

peptide bond is made where in the ribosome?

Answer 2

P site of the large subunit

Question 3

Why doesn’t translation go 3’ to 5’?

Answer 3

triphosphates aren’t stable and fall apart, so we would lose our energy, so it goes 5’-3’

Question 4

Does DNA alpha have exonuclease activity?

Answer 4

No

Question 5

Streptomycin

Answer 5

interferes with initiation

Question 6

what terminus is AA added to when a protein is formed?

Answer 6

free amine group for FIRST AA, after that it is added to carboxyl

Question 7

Tetracycline

Answer 7

blocks tRNA binding at the A-site

Question 8

Chloramphenicol

Answer 8

inhibits peptidyltransferase (inhibits peptide bond formation)

Question 9

Puromycin

Answer 9

structurally resembles tRNS and results in premature termination (acts as a release factor)

Question 10

Erythromycin

Answer 10

blocks the tunnel and inhibits translocation

Question 11

Rifampin

Answer 11

used to treat TB, inhibits initiation of RNA

synthesis

Question 12

tamoxifen

Answer 12

hormone receptor, a synthetic drug used to treat breast cancer and infertility in women

Question 13

cordyceptin

Answer 13

adenosine analogue, incorporated into RNA, and inhibits transcription/synthesis

Question 14

when collagen is secreted from the cell of origin it is in what form?

Answer 14

triple helix procollagen molecule

Question 15

the cleavage of procollagen to collagen by ECM proteolytic enzymes allows what event to occur?

Answer 15

assembly of large fibrils in extracellular space

Question 16

what base pairs are the 5’ end of an intron? What are the 3’ end?

Answer 16

GU, AG

Question 17

what site does RNA polymerase prefer to bind?

Answer 17

AG

Question 18

TF2H does what?

Answer 18

unwinds DNA at the transcription start point

Question 19

penicillin affects bacteria cell wall synthesis by?

Answer 19

acting as an irreversible inhibitor, it becomes covalently attached to the enzyme

Question 20

aminoacyl tRNA synthetases are responsible for?

Answer 20

transfer of AA to the 3’ end of tRNA and adenylation of AA resulting in the release of pyrophsphate

Question 21

tropcollagen molecules cross-link with one another to form?

Answer 21

collagen fibrils in the extracellular space

Question 22

What nucleotide base is actively removed by Base Excision Repair?

Answer 22

all of them

Question 23

proteins are synthesized?

Answer 23

in amino to carboxyl terminal ends

Question 24

tyrosine and tryptophan absorbs?

Answer 24

UV light at 280nm

Question 25

the propeptide ends of a procollagen molecule are?

Answer 25

cleaved from the molecule in the extracellular fluid

Question 26

fidelity

Answer 26

accuracy of genome duplication

Question 27

Pol alpha has ____ processivity and ____ fidelity

Answer 27

low and low

Question 28

Kozak sequence and shine delgarno found where?

Answer 28

Kozak= Euk

Shine Delgarno= Pro

Question 29

disorders caused by misfolded proteins?

Answer 29

neurodegenerative: parkinsons, huntingtons, alzheimers

Question 30

What AA can form sulfydryl groups?

Answer 30

cysteine

Question 31

What AA can form H-bonds via the carbonyl group or amide group?

Answer 31

Asparagine and Glutamine

Question 32

Name hydrophobic AA? How many phobic and how many philic?

Answer 32

9 hydrophobic
11 hydrophilic
VIP GAL Tries Trypin on Phyn

Question 33

What 3 AA can be charged and pronated determined by environment?

Answer 33

histidine, lysine, arginine

Question 34

What AA are typically phosphorylated or kinased?

Answer 34

serine, threonine, tyrosine