Exam 1 Random 2 Flashcards
splicing forms what linkage?
2’-5’
peptide bond is made where in the ribosome?
P site of the large subunit
Why doesn’t translation go 3’ to 5’?
triphosphates aren’t stable and fall apart, so we would lose our energy, so it goes 5’-3’
Does DNA alpha have exonuclease activity?
No
Streptomycin
interferes with initiation
what terminus is AA added to when a protein is formed?
free amine group for FIRST AA, after that it is added to carboxyl
Tetracycline
blocks tRNA binding at the A-site
Chloramphenicol
inhibits peptidyltransferase (inhibits peptide bond formation)
Puromycin
structurally resembles tRNS and results in premature termination (acts as a release factor)
Erythromycin
blocks the tunnel and inhibits translocation
Rifampin
used to treat TB, inhibits initiation of RNA
synthesis
tamoxifen
hormone receptor, a synthetic drug used to treat breast cancer and infertility in women
cordyceptin
adenosine analogue, incorporated into RNA, and inhibits transcription/synthesis
when collagen is secreted from the cell of origin it is in what form?
triple helix procollagen molecule
the cleavage of procollagen to collagen by ECM proteolytic enzymes allows what event to occur?
assembly of large fibrils in extracellular space
what base pairs are the 5’ end of an intron? What are the 3’ end?
GU, AG
what site does RNA polymerase prefer to bind?
AG
TF2H does what?
unwinds DNA at the transcription start point
penicillin affects bacteria cell wall synthesis by?
acting as an irreversible inhibitor, it becomes covalently attached to the enzyme
aminoacyl tRNA synthetases are responsible for?
transfer of AA to the 3’ end of tRNA and adenylation of AA resulting in the release of pyrophsphate
tropcollagen molecules cross-link with one another to form?
collagen fibrils in the extracellular space
What nucleotide base is actively removed by Base Excision Repair?
all of them
proteins are synthesized?
in amino to carboxyl terminal ends
tyrosine and tryptophan absorbs?
UV light at 280nm
the propeptide ends of a procollagen molecule are?
cleaved from the molecule in the extracellular fluid
fidelity
accuracy of genome duplication
Pol alpha has ____ processivity and ____ fidelity
low and low
Kozak sequence and shine delgarno found where?
Kozak= Euk
Shine Delgarno= Pro
disorders caused by misfolded proteins?
neurodegenerative: parkinsons, huntingtons, alzheimers
What AA can form sulfydryl groups?
cysteine
What AA can form H-bonds via the carbonyl group or amide group?
Asparagine and Glutamine
Name hydrophobic AA? How many phobic and how many philic?
9 hydrophobic
11 hydrophilic
VIP GAL Tries Trypin on Phyn
What 3 AA can be charged and pronated determined by environment?
histidine, lysine, arginine
What AA are typically phosphorylated or kinased?
serine, threonine, tyrosine
