week 6 - fungi Flashcards

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1
Q

fungi key features:
fungal cell

A
  • Typical eukaryotic cell structure
  • Except:
    o Ergosterol instead of cholesterol in plasma membrane (also in some protists)
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2
Q

fungi key features:
flagellated?

A
  • Very rarely flagellated
    o So often not motile
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3
Q

fungi key features:
cell wall

A

o Chitin
o Long chain polymer of N-acetylglucosamine
o Produced by chitin synthase (usually multiple paralogues in a genome)

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4
Q

characteristics of the fungal kingdom

A
  • A monophyletic group
  • One common ancestor
  • 100,000 species known, total probably >1.5 million
    o Likely millions of species we are unaware of
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5
Q

characteristics of the fungal kingdom:
chemoogranotrophs

A

o All derive energy by the breakdown of organic materia
o Same as us (not like plants)
o Do most of the digestion on the outside
 Secrete enzyme
 Then stuff wanted seeps through pores

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6
Q

characteristics of the fungal kingdom:
important as..

A

o Decomposers
 Have liganases (breaks down ligan in plants)
 Symbiosis – plant life into land (nutrients in soils), interaction and collaboration
o Food and drink industry
o Disease agents (esp. crops)

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7
Q

fungal lifestyles

A
  • Always yeast (Saccharomyces cerevisiae)
  • Always hyphal (Agaricus bispora)
    o Type of growth: Hyphal tip growth is characterized by the initial establishment of one growth site, which is followed by its continuous maintenance.
    o A hypha is a long, branching, filamentous structure of a fungus, oomycete, or actinobacterium. In most fungi, hyphae are the main mode of vegetative growth, and are collectively called a mycelium
  • A mixture (Candida albicans) = dimorphic
  • Reproduction is often asexual
    o Hyphal spread
    o Single cell budding – e.g. yeast
    o Asexual spores
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8
Q

filamentous fungi

A
  • Filamentous Fungal morphologies
    o Moulds
    o Mushrooms
  • Fruiting body for spore production
    o When conditions are right
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9
Q

filamentous fungi
- growth

A
  • Spore to hyphae = GERMINATION
  • GERM TUBE = first hyphae to emerge from a spore
  • Some spores have a predefined point for germination = GERM PORE
    o Where cell wall is weaker (on purpose)
  • Some swell before germ tube emerges
  • Environmental stimuli needed to initiate
    o In favourable conditions spores germinate (humidity, temp, etc)
    o Get swelling
    o Then one single new hyphae
    o Which keeps growing to make a mass of hyphae
    o Spores are a vehicle for transmission into new environments
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10
Q

filamentous fungi
consists of:

A
  • Consist of long filaments of cells joint end to end = hyphae
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11
Q

filamentous fungi:
main structure:

A

Hyphae – rigid tubes containing cytoplasm
o Interconnected compartments, not individual cells
o Interconnected by septum
 Extension of cell wall to add structure of hyphae

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12
Q

filamentous fungi:
growth

A

Filamentous fungi – growth
- Hyphae typically grow by apical growth:
o Extension at the tip
o New cells made at the front
o This is how it grows

New cells being formed at tip
In order to extent

Clustered area at tip of hyphae

Macrovesicles (glucan) with micro vesicles (chitin) inside

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13
Q

tropisms

A
  • Hyphal tips show tropism to a variety of substances
    o Nutrients
    o Amino acids
    o Volatile metabolites
    o Sex pheromones
    o light
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14
Q

Filamentous fungi
– four types of fungal hyphae

A
  • Septate or Coenocytic
  • Vegetative or Arial
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15
Q

Filamentous fungi
separate hyphae

A
  • Joint cells have distinct separations called septa, contain pores
    o more typical model
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16
Q

Filamentous fungi
coenocytic hyphae:

A
  • Hyphae consists of fused cells, multinucleated
    o More prominent in the older groups of fungi
    o No distinct markers between cells
    o (any negative mutations in one nuclei very easily compensated more, lots of nucelli as no proper separation)
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17
Q

Filamentous Fungi
– Septa
functions

A

o Structural support
o Allow movement of cell content between cells
 Whole organells (depends on species)
o Damage protection
- Made of similar things as the cell wall
- Structure and control varies between fungal species

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18
Q

Filamentous fungi – Growth

A
  • Mass of hyphae = mycelium (pural: mucelia)
19
Q

Filamentous fungi – Growth
vegetative hyphae

A
  • Grow along the surface
    o Sectreting enzymes and absorbing material
  • Obtain nutrients  filamentous = max surface area
20
Q

Filamentous fungi – Growth
Aerial hyphae

A
  • Stick up from the mycelium
    o Up and out of the nutrient
     Main purpose of spore production
     Into new environment
  • Spores for reproduction
21
Q

YEASTS

A
  • Non-filamentous, unicellular, oval
  • Facultative anaerobes
    o Ferment carbohydrates into alcohol and carbon in absence of oxygen
22
Q

YEAST
two types

A

budding
fission

23
Q

BUDDING YEAST

A
  • Divide by producing outgrowth called a bud
  • Bud enlarges and seperates from mother cell
    o With a septin ring
     The pinching off is drivien by spetin
     Septin ring forms and then it closes causing daughter cell to pinch off (alike actin and microtubules)
  • Asymmetric division
  • Example: Saccharomyces cerevisiae, Cryptococcus neoformans, Candida glabrata, Malassezia furfur
24
Q

FISSION YEAST

A
  • Division by elongation of mother cell and mitosis
  • Daughter cells separate by formation of septa in the centre
    o Actin rings
    o Actin ring closes off and separate cells (quick snap)
  • Example: Schizosaccharomyces pombe
25
Q

DIMORPHIC FUNGI

A
  • Grow as multicellular hyphae OR unicellular yeast
    o Depending on environmental conditions
26
Q

Dimorphism

A
  • Some pathogens of humans exhibit dimorphism
  • Plays an important role in how and why they cause disease
27
Q

Dimorphism and disease

A

Candida albicans:
environmental change becomes a hyphae:  now causes a problem

Histoplasma capsulatum:
budding form causes a switch to a pathogen

28
Q

fungi summary:
fungal morphologies

A

o Yeast, filamentous and dimorphic

29
Q

fungi summary:
fungal growth patterns

A

o Germination of spores
o Apical growth of hyphae
o Septate vs coenocytic
o Budding vs fission

30
Q

fungi summary:
the fungal phyla

A

o Determined by spore containing structures

31
Q

THE FUNGAL PHYLA

A
  • Characterised by the structure of the spore containing organ
32
Q

THE FUNGAL PHYLA:
Chytridiomycota

A
  • Most primitive fungi
  • Approx. 1000 species
  • Many chytrids are aquatic
  • Motile
    o Zoospores in Zoosporangium
  • Chytridiomycosis
    o Batrachochytrium dendrobatids
33
Q

THE FUNGAL PHYLA:
Mucormycota

A
  • Approx.. 1000 species
  • Coenocytic hyphae
  • Food spoilage
  • Mucormycosis

have zygosporangium with zygospores inside

34
Q

THE FUNGAL PHYLA:
Glomeromycota

A
  • Approx. 230 species
  • Arbuscular mycorrhizas (>80% plants)
  • Coenocytic hyphae
  • Fungus helps plant to capture nutrients such as:
    o phosphorus, sulphur and nitrogen
  • Plant provides carbohydrates
  • Obligate plant symbionts

e.g. Glomus mosseae

  • Sexual lifecycle unknown
    o Form Glomersopores from hyphae tips
35
Q

THE FUNGAL PHYLA:
Ascomycota

A
  • Approx. 65, 000 species
  • Can form ascocarp fruiting body septate hyphae
  • Example: Morchella, Gailella
  • Asexual = conidia on a conidiophore

Sexual = Ascus containing ascospores

36
Q

THE FUNGAL PHYLA
Ascomycota
good

A

o Saccharmyces cerevisiae
o Saccharomyces carlsberensis
o Penicillum roqueforti
o Pencillium chrusogenum

37
Q

THE FUNGAL PHYLA
Ascomycota
bad (cause disease)

A

o Candida albicans
o Asperguillus fumigatus
o Histoplasma capsulatum
o Coccidioides immitis
o Magnaporthe oryzae
o Truchophyton rubrum
o Malassezia glabosa

38
Q

THE FUNGAL PHYLA
Basidiomycota

A
  • > 30,000 species
  • Basidium with basidiospores
  • Can form basiocarp (fruiting body) = MUSHROOM
  • Mycorrhizal fungi too

Mantle = think layer of fungal cells grows to tip of roots
Hartig net = fungal cells growing between epidermis cells
About 2% of plant species

  • Examples:
    o Amanita muscaria, Amanita phalloides, Coprinopsis atramentaria (all mushrooms
    o Cryptococcus neoformans
39
Q

fungal morphology and growth:
the structure of fungal cells

A

o Nucleus
o Membrane
o Chitosomes
o Cell wall

40
Q

fungal morphology and growth:
the fungal nucleus

A
  • Ploidy
  • Most haploid with 6-20 chromosomes
  • Others alternate between haploid and diploid
  • Unique features of fungal nuclei:
    o Membrane remains intact during mitosis
    o No clear metaphase plate
41
Q

fungal morphology and growth:
fungal cytoplasmic organelles

A
  • Plasma membrane
    o Phospholipid bilayer
     Anchorage for enzymes/proteins such as chitin synthases or glucan synthases
     Contains ergosterol
  • Chitosomes
    o Microvesicle for chitin synthesis
42
Q

fungal morphology and growth:
fungal cell wall

A
  • Structural barrier
  • Environmental interface
    o Protects against osmotic lysis
    o Contain pigments for protection
    o Mediates interaction with other organisms
43
Q
A