Week 2: Worksheet

Question 1

Concentration of virus formula (PFU/mL)

Answer 1

plaques x (dilution factor positive) x 10

Question 2

Which of the following statements is incorrect?
a. The infectious cycle and virus reproduction are synonymous
b. A susceptible cell has a functional receptor for a given virus
c. A cell that is resistant to infection has the viral receptor but the virus cannot be
reproduced in it
d. Virus can be reproduced in a permissive cell
e. A susceptible and permissive cell is the only cell in which a virus can enter and be
reproduced

Answer 2

c. A cell that is resistant to infection has the viral receptor but the virus cannot be
reproduced in it

Question 3

Calculate the amount of virus (PFU/ml) you will need if you infect 500,000 cells (5 x
105) at an MOI of 5.

Answer 3

2.5 x 106 PFU/mL

Question 4

MOI formula

Answer 4

MOI= (PFU/mL)/ # of cells

Question 5

P(1) = ?

Answer 5

me^-m = m x (1/e^m)

Question 6

P(0) = ?

Answer 6

e^-m = 1/e^m or

Question 7

m= ?

Answer 7

-ln(P0)

Question 8

Viral assay method table

Answer 8

IPAD