Week 2: Microscopy Flashcards

1
Q

what are the three principles of microscopy?

A

magnification
resolution
contrast

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2
Q

what magnification should be used to focus my sample first? then what?

A

10X, then move to higher magnification

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3
Q

what height should i start at with the 10X lens?

A

maximum height, then move the stage down until it comes into focus

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4
Q

100X always uses…

A

oil

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5
Q

oil is always used with…

A

100X

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6
Q

what magnification should i use on a unstained human red blood cell?

A

10X

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7
Q

what contrast should i use on an unstained human red blood cell?

A

BF

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8
Q

what resolution should i use on a unstained human red blood cell?

A

air

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9
Q

what magnification should i use on a capsule stained bacteria cell?

A

100X

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10
Q

what contrast should i use on a capsule stained bacteria cell?

A

DF

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11
Q

what resolution should i use on a capsule stained bacteria cell?

A

oil

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12
Q

what magnification should i use for eukaryotic cells?

A

40X

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13
Q

what magnification should i use for bacterial cells?

A

100X

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14
Q

when to use bright field?

A

live, no stain

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15
Q

when to use dark field?

A

capsule

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16
Q

when to use phase contrast?

A

unstained/colorless cells because staining provides a contrast

17
Q

what resolution with 100X?

18
Q

what resolution with 10X?

19
Q

what resolution with 40X?

20
Q

when should i avoid bright field?

A

when viewing unstained wet mounts

21
Q

how do organisms appear in dark field?

A

bright and glowing against a dark background

22
Q

what magnification can and can’t we use with dark field?

A

CAN: 10X and 40X
CAN’T: 100X

23
Q

what is the best contrast setting for unstained live organisms?

A

phase contrast

24
Q

phase contrast is the best setting for…

A

unstained live organisms

25
match phase ring to objective: PH1
10X
26
match phase ring to objective: PH2
r40X
27
match phase ring to objective: PH3
100X
28
match objective to phase ring: 100X
PH3
29
match objective to phase ring: 40X
PH2
30
match objective to phase ring: 10X
PH1
31
what contrast to use for 100X?
BF/phase contrast for 100X, not DF
32
how to use oil immersion lens?
1. 100X needs oil 2. focus sample in 40X 3. go back to 10X and put oil onto the slide. 4. move 100X into place