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Microbio Lab > Week 2: Microscopy > Flashcards

Week 2: Microscopy Flashcards

1
Q

what are the three principles of microscopy?

A

magnification
resolution
contrast

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2
Q

what magnification should be used to focus my sample first? then what?

A

10X, then move to higher magnification

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3
Q

what height should i start at with the 10X lens?

A

maximum height, then move the stage down until it comes into focus

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4
Q

100X always uses…

A

oil

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5
Q

oil is always used with…

A

100X

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6
Q

what magnification should i use on a unstained human red blood cell?

A

10X

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7
Q

what contrast should i use on an unstained human red blood cell?

A

BF

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8
Q

what resolution should i use on a unstained human red blood cell?

A

air

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9
Q

what magnification should i use on a capsule stained bacteria cell?

A

100X

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10
Q

what contrast should i use on a capsule stained bacteria cell?

A

DF

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11
Q

what resolution should i use on a capsule stained bacteria cell?

A

oil

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12
Q

what magnification should i use for eukaryotic cells?

A

40X

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13
Q

what magnification should i use for bacterial cells?

A

100X

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14
Q

when to use bright field?

A

live, no stain

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15
Q

when to use dark field?

A

capsule

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16
Q

when to use phase contrast?

A

unstained/colorless cells because staining provides a contrast

17
Q

what resolution with 100X?

A

oil

18
Q

what resolution with 10X?

A

air

19
Q

what resolution with 40X?

A

air

20
Q

when should i avoid bright field?

A

when viewing unstained wet mounts

21
Q

how do organisms appear in dark field?

A

bright and glowing against a dark background

22
Q

what magnification can and can’t we use with dark field?

A

CAN: 10X and 40X
CAN’T: 100X

23
Q

what is the best contrast setting for unstained live organisms?

A

phase contrast

24
Q

phase contrast is the best setting for…

A

unstained live organisms

25
Q

match phase ring to objective: PH1

A

10X

26
Q

match phase ring to objective: PH2

A

r40X

27
Q

match phase ring to objective: PH3

A

100X

28
Q

match objective to phase ring: 100X

A

PH3

29
Q

match objective to phase ring: 40X

A

PH2

30
Q

match objective to phase ring: 10X

A

PH1

31
Q

what contrast to use for 100X?

A

BF/phase contrast for 100X, not DF

32
Q

how to use oil immersion lens?

A
  1. 100X needs oil
  2. focus sample in 40X
  3. go back to 10X and put oil onto the slide.
  4. move 100X into place

Microbio Lab (14 decks)

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