Week 1: Introduction to laboratory practice and procedures

Question 1

purpose of streak plate technique?

Answer 1

obtain a pure culture

Question 2

what is a pure culture?

Answer 2

population of bacteria derived from a single cell and therfore genetically alike

Question 3

what’s the idea behind a streak plate?

Answer 3

increase the dilution of organisms so that individual bacteria are deposited far enough apart to be able to grow into isolated colonies

Question 4

what does autoclave mean? why do we do it?

Answer 4

high temp and pressure.
sterilize media.

Question 5

what do we use to sterilize wire loops? spreaders?

Answer 5

flame
alcohol disinfection

Question 6

what does sizzling mean in a streak plate technique?

Answer 6

death

Question 7

what is spread plate technique used for?

Answer 7

quantitation of samples

Question 8

how is spread plate technique done?

Answer 8

make various dilutions, plate dilutions and isolate colonies using a spreader. count colonies and mathematically calculate how many cells in the original sample.

Question 9

the _______________ of the individual colony can be used to…

Answer 9

physical characteristics of the individual colony can be used to help distinguish it from different isolates

Question 10

what does the P stand for on the micropipette?

Answer 10

maximum volume that the pipette can hold in ul

Question 11

P20: 01.5

Answer 11

1.5ul

Question 12

P20: 15.0

Answer 12

15 ul

Question 13

P200: 020

Answer 13

20 ul

Question 14

P200: 200

Answer 14

200 ul

Question 15

P1000: 0.20

Answer 15

200 ul

Question 16

P1000: 1.00

Answer 16

1000 ul