Lab 2 Pre-Lab Flashcards
What is the maximum magnification on these microscopes?
1000X (aka 10X x 100X)
Which is the only lens that needs oil?
100X oil immersion
What to do to aid with viewing?
switch to dark field (DF) or Phase contrast
If you use the DF setting…
Increase the light to maximum and bring the light condenser all the way up
How to increase and decrease contrast?
Open and close the iris diaphragm
Which ring to use with 40X lens?
PH2
Never remove slide from 40X or 100X…
Without lowering stage or you will scratch the lens.
Do not play with the ….. when 100X is in place
Coarse focus screw
Describe peritrichous flagella
Flagella all around the cell
Describe atrichous flagella
No flagella
Describe monotrichous flagella
One flagella
Describe amphitrichous flagella
One flagella on either end
Describe lophotrichous flagella
A few flagella on one end
Alternative motility to flagellar movement?
Gliding motility, move slowly over solid surfaces
Can we see bacterial flagella in our lab? How about eukaryotic flagella?
No; yes.
what is true motility?
microorganisms moving in separate directions
what is brownian motion?
movement where the cells appear to remain in one position but they shake in place
what is flow?
cells appear to be swept in one direction
3 main methods to see microbial motility in our lab?
- Wet mount
- Hanging drop method
- Motility agar
What is TTC?
A colorless dye that when reduced by bacterial cells, produces formazan.
What is formazan?
An insoluble red pigment.
Where does formazan form?
Only in the area of bacterial growth
What does TTC stand for?
Triphenyltetrazolium chloride
