Lab 2 Pre-Lab

Question 1

What is the maximum magnification on these microscopes?

Answer 1

1000X (aka 10X x 100X)

Question 2

Which is the only lens that needs oil?

Answer 2

100X oil immersion

Question 3

What to do to aid with viewing?

Answer 3

switch to dark field (DF) or Phase contrast

Question 4

If you use the DF setting…

Answer 4

Increase the light to maximum and bring the light condenser all the way up

Question 5

How to increase and decrease contrast?

Answer 5

Open and close the iris diaphragm

Question 6

Which ring to use with 40X lens?

Answer 6

PH2

Question 7

Never remove slide from 40X or 100X…

Answer 7

Without lowering stage or you will scratch the lens.

Question 8

Do not play with the ….. when 100X is in place

Answer 8

Coarse focus screw

Question 9

Describe peritrichous flagella

Answer 9

Flagella all around the cell

Question 10

Describe atrichous flagella

Answer 10

No flagella

Question 11

Describe monotrichous flagella

Answer 11

One flagella

Question 12

Describe amphitrichous flagella

Answer 12

One flagella on either end

Question 13

Describe lophotrichous flagella

Answer 13

A few flagella on one end

Question 14

Alternative motility to flagellar movement?

Answer 14

Gliding motility, move slowly over solid surfaces

Question 15

Can we see bacterial flagella in our lab? How about eukaryotic flagella?

Answer 15

No; yes.

Question 16

what is true motility?

Answer 16

microorganisms moving in separate directions

Question 17

what is brownian motion?

Answer 17

movement where the cells appear to remain in one position but they shake in place

Question 18

what is flow?

Answer 18

cells appear to be swept in one direction

Question 19

3 main methods to see microbial motility in our lab?

Answer 19

1. Wet mount
2. Hanging drop method
3. Motility agar

Question 20

What is TTC?

Answer 20

A colorless dye that when reduced by bacterial cells, produces formazan.

Question 21

What is formazan?

Answer 21

An insoluble red pigment.

Question 22

Where does formazan form?

Answer 22

Only in the area of bacterial growth

Question 23

What does TTC stand for?

Answer 23

Triphenyltetrazolium chloride