Week 2 (Lab Safety)

Question 1

SOCC

Answer 1

Safe, Organized, Clean, Considerate

Question 2

Proper lab attire

Answer 2

-tie back long hair
-closed toe shoes are required
-pants must cover all skin from waste down (even when sitting)
-goggles must be worn at all times in
-full socks that cover entire foot and ankle
-long-sleeved shirt (no skin showing from neck down)
-remove any loose accessories such as jewelry
-wear lab coat

Question 3

Glove policy

Answer 3

-gloves are not required unless procedure calls for it
-if gloves are worn, avoid touching shared equipment (balances, magnets, retrievers, etc) with gloved hands
-never use gloved hand to take down notes (anything that leaves lab must be chemical free)

Question 4

Glove removal

Answer 4

turn gloves inside out, touching only inside of gloves

Question 5

When can you enter lab?

Answer 5

When instructor is present

Question 6

Chemical fume hoods

Answer 6

-where all experiments are performed
-where all chemicals and glassware are stored
-hood sashes should not be opened too wide, or else chemical fumes will escape and alarm will sound
-hood doors should not be obstructed by electrical cords
-never allow head to enter hood opening

Question 7

Where should computer/lab manual be kept?

Answer 7

on the side bench

Question 8

Guidelines related to electronics

Answer 8

-cell phones and headphones should not be used in labs and should be kept away
-must be able to listen to lab AI and work without distraction

Question 9

number and location of safety showers

Answer 9

2, near exits, used after chemical spills on clothes/self, stay under shower for at least 15 minutes

Question 10

number and location of eye washes

Answer 10

2, in the common area near entrance and at sink near AI hood, used for chemical spills in eyes, flush eyes for at least 15 minutes

Question 11

number and location of fire extinguishers

Answer 11

2, near exits

Question 12

PASS acronym

Answer 12

Pull the pin, Aim the hose, Squeeze the lever, Sweep the hose side to side

Question 13

number and location of spill kits

Answer 13

1, cabinet under AI hood

Question 14

Types of spill kits

Answer 14

Acid spill kit (red), Base spill kit (blue), Organic solvent spill kit (purple)

Question 15

Brush and dust pan

Answer 15

Used for sweeping up spill kit powder and broken glass

Question 16

Types and locations of faucets/sinks

Answer 16

-On central lab benches
-Central rubber faucet: deionized water (solvent for almost every reaction/solution)
-Left faucet: pressurized tap water
-Right faucet: regular tap water

Question 17

Cleaning glassware procedure

Answer 17

-rinse with tap water and detergent
-final rinse should be with deionized water

Question 18

Fire alarm procedure

Answer 18

-All students must evacuate lab
-all electronic equipment (hot plates and stir plates) must be turned off/unplugged
-do not pick up belongings before leaving

Question 19

Types of disposal bins

Answer 19

-Regular trash bin: paper towels, face masks
-Contaminated solids receptacle: chemically contaminated paper towels, gloves
-Broken glass bin: broken glass (chemical free)
-Chemical waste container: leftover chemicals after experiment, found in AI hood (use funnel to pour waste into waste container)
-Pipet waste container: disposable pipets

Question 20

How is fire put out in lab?

Answer 20

small fires can be put out using a beaker, fire blanket can be used if fire spreads to clothes, AI can use fire extinguisher if fire is large

Question 21

Safety Data Sheet (SDS)

Answer 21

should be consulted for each chemical used to determine if there are any special recommendations for exposure situations

Question 21

National Fire Protection Association (NFPA) Diamond

Answer 21

Top (red) diamond: flammability
Left (blue) diamond: health hazard info
Right (yellow) diamond: reactivity hazard info
Bottom (white) diamond: special hazard info

Question 22

Spill cleaning procedure

Answer 22

-get spill kit and alert AI
-stop spread of liquid by surrounding spill with absorbent from kit, then cover whole spill with absorbent
-scoop up absorbent with scoop pan and put absorbent into plastic bag
-tie off plastic bag and place into another plastic bag, label it, then dispose in solid waste container
-for acids, use baking soda to neutralize spill
-for bases, use citric acid to neutralize spill
-hydrofluoric acid and mercury require special cleaning instructions
-wipe down area after cleaning

Question 23

Contents of spill kit

Answer 23

-material to absorb spill, bags to dispose of absorbent

Question 24

How to avoid contamination

Answer 24

-never return chemicals back to reagent bottle after removing them
-do not use lab implements, such as spatulas, in different reagent bottles without cleaning the implement between uses
-pour chemical into beaker before pouring into more delicate glassware such as burette

Question 25

Good chemical storage location

Answer 25

cool, dry, dark, like chemical storage cabinet (do not use fume hood for longterm storage of chemicals)