Week 2 (Lab Safety) Flashcards
SOCC
Safe, Organized, Clean, Considerate
Proper lab attire
-tie back long hair
-closed toe shoes are required
-pants must cover all skin from waste down (even when sitting)
-goggles must be worn at all times in
-full socks that cover entire foot and ankle
-long-sleeved shirt (no skin showing from neck down)
-remove any loose accessories such as jewelry
-wear lab coat
Glove policy
-gloves are not required unless procedure calls for it
-if gloves are worn, avoid touching shared equipment (balances, magnets, retrievers, etc) with gloved hands
-never use gloved hand to take down notes (anything that leaves lab must be chemical free)
Glove removal
turn gloves inside out, touching only inside of gloves
When can you enter lab?
When instructor is present
Chemical fume hoods
-where all experiments are performed
-where all chemicals and glassware are stored
-hood sashes should not be opened too wide, or else chemical fumes will escape and alarm will sound
-hood doors should not be obstructed by electrical cords
-never allow head to enter hood opening
Where should computer/lab manual be kept?
on the side bench
Guidelines related to electronics
-cell phones and headphones should not be used in labs and should be kept away
-must be able to listen to lab AI and work without distraction
number and location of safety showers
2, near exits, used after chemical spills on clothes/self, stay under shower for at least 15 minutes
number and location of eye washes
2, in the common area near entrance and at sink near AI hood, used for chemical spills in eyes, flush eyes for at least 15 minutes
number and location of fire extinguishers
2, near exits
PASS acronym
Pull the pin, Aim the hose, Squeeze the lever, Sweep the hose side to side
number and location of spill kits
1, cabinet under AI hood
Types of spill kits
Acid spill kit (red), Base spill kit (blue), Organic solvent spill kit (purple)
Brush and dust pan
Used for sweeping up spill kit powder and broken glass
Types and locations of faucets/sinks
-On central lab benches
-Central rubber faucet: deionized water (solvent for almost every reaction/solution)
-Left faucet: pressurized tap water
-Right faucet: regular tap water
Cleaning glassware procedure
-rinse with tap water and detergent
-final rinse should be with deionized water
Fire alarm procedure
-All students must evacuate lab
-all electronic equipment (hot plates and stir plates) must be turned off/unplugged
-do not pick up belongings before leaving
Types of disposal bins
-Regular trash bin: paper towels, face masks
-Contaminated solids receptacle: chemically contaminated paper towels, gloves
-Broken glass bin: broken glass (chemical free)
-Chemical waste container: leftover chemicals after experiment, found in AI hood (use funnel to pour waste into waste container)
-Pipet waste container: disposable pipets
How is fire put out in lab?
small fires can be put out using a beaker, fire blanket can be used if fire spreads to clothes, AI can use fire extinguisher if fire is large
Safety Data Sheet (SDS)
should be consulted for each chemical used to determine if there are any special recommendations for exposure situations
National Fire Protection Association (NFPA) Diamond
Top (red) diamond: flammability
Left (blue) diamond: health hazard info
Right (yellow) diamond: reactivity hazard info
Bottom (white) diamond: special hazard info
Spill cleaning procedure
-get spill kit and alert AI
-stop spread of liquid by surrounding spill with absorbent from kit, then cover whole spill with absorbent
-scoop up absorbent with scoop pan and put absorbent into plastic bag
-tie off plastic bag and place into another plastic bag, label it, then dispose in solid waste container
-for acids, use baking soda to neutralize spill
-for bases, use citric acid to neutralize spill
-hydrofluoric acid and mercury require special cleaning instructions
-wipe down area after cleaning
Contents of spill kit
-material to absorb spill, bags to dispose of absorbent
How to avoid contamination
-never return chemicals back to reagent bottle after removing them
-do not use lab implements, such as spatulas, in different reagent bottles without cleaning the implement between uses
-pour chemical into beaker before pouring into more delicate glassware such as burette
Good chemical storage location
cool, dry, dark, like chemical storage cabinet (do not use fume hood for longterm storage of chemicals)
